Protein-matrix microlens array diffraction device and method for preparing the same

Patent metadata

Field	Value
Publication number	US-11221437-B2
Application number	US-202117323415-A
Country	US
Kind code	B2
Filing date	May 18, 2021
Priority date	Sep 18, 2019
Publication date	Jan 11, 2022
Grant date	Jan 11, 2022

Abstract

Provided are a protein-matrix microlens array diffraction device and a preparation method thereof. The protein-matrix microlens array diffraction device includes a matrix of a protein crystal. A largest side of the protein crystal has a length of 100 to 500 μm, a surface of the protein crystal where the largest side is located is processed to have an array of microlens-like protrusions, a distance p between two adjacent microlens-like protrusions of the array of microlens-like protrusions is in a range of 10 to 100 μm, a diameter d of the microlens-like protrusion is in a range of 2 to 10 μm, and a height h of the microlens-like protrusion is in a range of 0.05 to 2 μm.

First claim

What is claimed is:

1. A protein-matrix microlens array diffraction device, characterized in that the protein-matrix microlens array diffraction device comprises a matrix of a protein crystal,
a largest side of the protein crystal has a length of 100 to 500 μm,
a surface of the protein crystal where the largest side is located is processed to have an array of microlens-like protrusions,
a distance p between two adjacent microlens-like protrusions of the array of microlens-like protrusions is in a range of 10 to 100 μm,
a diameter d of the microlens-like protrusion is in a range of 2 to 10 μm, and
a height h of the microlens-like protrusion is in a range of 0.05 to 2 μm.

2. The protein-matrix microlens array diffraction device according to claim 1 , wherein the protein crystal is an HEWL crystal.

3. The protein-matrix microlens array diffraction device according to claim 1 , wherein the distance p between two adjacent microlens-like protrusions of the array of microlens-like protrusions is 10 μm.

4. The protein-matrix microlens array diffraction device according to claim 1 , wherein the diameter d of the microlens-like protrusion is 3 μm.

5. The protein-matrix microlens array diffraction device according to claim 1 , wherein the height h of the microlens-like protrusion is 1 μm.

6. A method for preparing a protein-matrix microlens array diffraction device, comprising the following steps:
(1) preparing a protein crystal, comprising:
(1-1) mixing a protein solid with a solution having a concentration of a salt, comprising sodium chloride and sodium acetate, of 2% to 5% by mass to obtain a protein solution, wherein a mass fraction of the protein in the protein solution is 1 to 3 times a mass fraction of a saturated solution, and the protein solution is adjusted to have a pH of 4 to 6; and
(1-2) placing the protein solution obtained from step (1-1) in a container and leaving the protein solution to stand at a room temperature for a period of 6 to 24 h, wherein during the period water of the protein solution is naturally evaporated, thereby obtaining the protein crystal,

and
(2) emitting a femtosecond laser on a surface of the protein crystal obtained in step (1), wherein the femtosecond laser has a pulse duration of 35 to 120 fs, a single pulse energy of the femtosecond laser is half of a protein ablation threshold, and the femtosecond laser has a repetition frequency of 1 to 1000 Hz.

7. The method according to claim 6 , wherein the protein solution is naturally evaporated at a temperature of 23° C. and a relative humidity of 30% for 24 h.

Assignees

• Univ Tsinghua

Inventors

• Jiang Lan
• Yan Jianfeng
• Yu Jiachen

Classifications

• C30B7/04

  using aqueous solvents · CPC title

• C30B29/58

  Macromolecular compounds · CPC title

• C12Y302/01017

  Lysozyme (3.2.1.17) · CPC title

• C07K2299/00

  Coordinates from 3D structures of peptides, e.g. proteins or enzymes · CPC title

• G02B5/1866 Primary

  Transmission gratings characterised by their structure, e.g. step profile, contours of substrate or grooves, pitch variations, materials (G02B5/1809, G02B5/1828, G02B5/1833, G02B5/1838 and G02B5/1847 take precedence) · CPC title