Microfluidic channel structure and fabrication method thereof, microfluidic detecting device and detecting method thereof

Patent metadata

Field	Value
Publication number	US-11219894-B2
Application number	US-201916647386-A
Country	US
Kind code	B2
Filing date	Sep 24, 2019
Priority date	Nov 6, 2018
Publication date	Jan 11, 2022
Grant date	Jan 11, 2022

Abstract

A microfluidic channel structure and a fabrication method thereof, a microfluidic detecting device and a detecting method thereof are disclosed. The microfluidic channel structure includes a support portion; a foundation portion, provided on the support portion and including a first foundation and a second foundation spaced apart from each other; and a channel defining portion, provided on a side of the foundation portion that is away from the support portion and including a first channel layer and a second channel layer, the first channel layer covering the first foundation and the second channel layer covering the second foundation have a gap therebetween to define a microfluidic channel; and the first channel layer and the second channel layer are made of a same material.

First claim

The invention claimed is:

1. A microfluidic channel structure, comprising:
a support portion;
a foundation portion on the support portion, the foundation portion comprising a first foundation and a second foundation spaced apart from each other, the first foundation and the second foundation having an extension direction parallel to a surface of the support portion;
a channel defining portion on a side of the foundation portion away from the support portion, the channel defining portion comprising a first channel layer and a second channel layer, the first channel layer covering the first foundation, the second channel layer covering the second foundation, a gap between the first channel layer and the second channel layer defining a microfluidic channel, and the first channel layer and the second channel layer being made of a same material; and
an optical film layer between the support portion and the foundation portion, wherein the optical film layer comprises an optical signal transmission region and an optical signal shielding region, and wherein an orthographic projection of the optical signal transmission region on the support portion falls within orthographic projections of side faces of the first foundation and the second foundation on the support portion, so that an excitation signal passes through the optical signal transmission region and reaches the microfluidic channel.

2. The microfluidic channel structure according to claim 1 , wherein the channel defining portion further comprises:
a first channel wall, covering a side face of the first foundation that faces towards the microfluidic channel;
a second channel wall, covering a side face of the second foundation that faces towards the microfluidic channel;
the first channel wall and the second channel wall are opposite to each other and serve as two opposite side walls of the microfluidic channel.

3. The microfluidic channel structure according to claim 2 , wherein the channel defining portion further comprises: a third channel layer connecting the first channel wall and the second channel wall, wherein the third channel layer covers the support portion and serves as a bottom portion of the microfluidic channel.

4. The microfluidic channel structure according to claim 3 , wherein the first channel layer, the second channel layer, the first channel wall, the second channel wall and the third channel layer are connected with one another and are made of a same light-transmitting material.

5. The microfluidic channel structure according to claim 1 , wherein the first channel layer and the second channel layer are in contact with each other, so that the microfluidic channel has an enclosed chamber.

6. The microfluidic channel structure according to claim 1 , wherein the first channel layer and the second channel layer are not in contact with each other, so that the microfluidic channel has an opening on a side that is away from the support portion.

7. The microfluidic channel structure according to claim 1 , wherein the foundation portion further comprises a light reflective layer on side faces of the first foundation and the second foundation that face towards the microfluidic channel, wherein the light reflective layer is configured to reflect the excitation signal onto a sample to be detected.

8. The microfluidic channel structure according to claim 1 , wherein each of the first foundation and the second foundation is formed by sequentially stacking two or more foundation layers.

9. The microfluidic channel structure according to claim 1 , wherein an orthographic projection of the microfluidic channel on the support portion falls within an orthographic projection of the optical signal shielding region on the support portion.

10. The microfluidic channel structure according to claim 1 , wherein the microfluidic channel has an extension direction parallel to the surface of the support portion, and the extension direction of the microfluidic channel is parallel to the extension direction of the first foundation and the second foundation; in a plane perpendicular to the extension direction of the microfluidic channel, the microfluidic channel has a cross section that is tapered in a direction from the support portion to the foundation portion.

11. A microfluidic detecting device, comprising:
a microfluidic channel structure, the microfluidic channel structure, comprising:
a support portion;
a foundation portion on the support portion, the foundation portion comprising a first foundation and a second foundation spaced apart from each other, the first foundation and the second foundation having an extension direction parallel to a surface of the support portion;
a channel defining portion on a side of the foundation portion away from the support portion, the channel defining portion comprising a first channel layer and a second channel layer, the first channel layer covering the first foundation, the second channel layer covering the second foundation, a gap between the first channel layer and the second channel layer defining a microfluidic channel, and the first channel layer and the second channel layer being made of a same material; and
an optical film layer between the support portion and the foundation portion, wherein the optical film layer comprises an optical signal transmission region and an optical signal shielding region, and wherein an orthographic projection of the optical signal transmission region on the support portion falls within orthographic projections of side faces of the first foundation and the second foundation on the support portion, so that an excitation signal passes through the optical signal transmission region and reaches the microfluidic channel,
wherein the microfluidic channel is provided therein with a sample to be detected, and the microfluidic detecting device further comprises:
at least one signal transmitter, configured to generate the excitation signal, the excitation signal irradiating the sample to be detected to excite the sample to be detected to generate a sample signal; and
at least one signal detector, configured to receive and detect the sample signal.

12. The microfluidic detecting device according to claim 11 , wherein the at least one signal transmitter comprises a first signal transmitter in the foundation portion; the at least one signal detector is provided on a side of the channel defining portion that is away from the support portion, on a side of the support portion that is away from the channel defining portion, or in the support portion.

13. The microfluidic detecting device according to claim 12 , wherein the at least one signal transmitter further comprises a second signal transmitter on the side of the channel defining portion that is away from the support portion.

14. The microfluidic detecting device according to claim 11 , wherein one of the at least one signal transmitter and the at least one signal detector is in the first foundation of the foundation portion, and the other is provided in the second foundation of the foundation portion.

15. The microfluidic detecting device according to claim 14 , wherein the at least one signal transmitter comprises a first signal transmitter configured to serve as the first foundation; the at least one signal detector comprises a first signal detector and a second signal detector; the first signal detector is configured to serve as the second foundation; and the second signal detector is located on a side of the microfluidic channel that is away from the support portion.

16. The microfluidic detecting device according to claim 11 , wherein

Assignees

• Boe Technology Group Co Ltd

Inventors

• Ma Xiaochen
• Yuan Guangcai
• Ning ce
• Gu Xin
• Hu hehe

Classifications

• G01N2021/056

  Laminated construction · CPC title

• B01L3/502707 Primary

  characterised by the manufacture of the container or its components · CPC title

• B01L2300/168

  Specific optical properties, e.g. reflective coatings · CPC title

• G01N35/00

  Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor · CPC title

• B01L2200/10

  Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept · CPC title