Microwell plate for high-throughput detection and application thereof
US-2016348148-A1 · Dec 1, 2016 · US
US11213791B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11213791-B2 |
| Application number | US-201515763064-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 22, 2015 |
| Priority date | Oct 23, 2015 |
| Publication date | Jan 4, 2022 |
| Grant date | Jan 4, 2022 |
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A microporous structure includes an array of nano wires and a coating about the nano wires of the array. The coating defines pores between the nano wires.
Opening claim text (preview).
What is claimed is: 1. An apparatus comprising: a two-dimensional array of columnar nano wires; a substrate from which each of the columnar nano wires project; and a coating about the columnar nano wires, the coating defining pores between the columnar nano wires to form a microporous structure, wherein the substrate comprises: a first channel containing the two-dimensional array of columnar nano wires, the columnar nano wires of two-dimensional array are perpendicular to the substrate, and each of the columnar nano wires projecting from a floor of the first channel; and wherein the apparatus further comprises: a second channel formed in the substrate and having a second floor; and a second two-dimensional array of columnar nano wires, the columnar nano wires of the second two-dimensional array are perpendicular to the substrate, and each of the columnar nano wires of the second two-dimensional array projecting from the second floor the second channel; and a second coating about the columnar nano wires of the second two-dimensional array, the second coating defining second pores between the columnar nano wires of the second two-dimensional array to form a second microporous structure, wherein the coating about the columnar nano wires of the two-dimensional array has a first thickness and wherein the second coating about the columnar nano wire of the second two-dimensional array has a second thickness greater than the first thickness. 2. The apparatus of claim 1 further comprising an organic phase supported within the pores to form a supported liquid membrane. 3. The apparatus of claim 1 wherein the substrate holds the columnar nano wires of the first and/or second two-dimensional array in place relative to one another. 4. The apparatus of claim 1 , wherein the coating joins the columnar nano wires of first and/or second two-dimensional array. 5. The apparatus of claim 1 , wherein the columnar nano wires of first and/or second two-dimensional array have bent portions that are. 6. The apparatus of claim 1 , wherein the microporous structure has a thickness corresponding to a height of the columnar nano wires. 7. The apparatus of claim 1 wherein each of the columnar nano wires of the first and/or second two-dimensional array has a first end in contact with the substrate and a second end free of the substrate. 8. A method comprising: forming an array of nano wires upon a substrate comprising a first channel and a second channel; coating a first plurality of the nano wires of the array within the first channel with a first thickness of a coating to form a first plurality of pores between the first plurality of nano wires, wherein the first plurality of nano wires is projected from a floor of the first channel and perpendicular to the substrate; and coating a second plurality of the nano wires of the array within the second channel with a second thickness of the coating to form a second plurality of pores between the second plurality of nano wires, wherein the second plurality of nano wires is projected from a second floor of the second channel and perpendicular to the substrate, and wherein the second thickness is greater than the first thickness. 9. The method of claim 8 , wherein the nano wires are coated such that coatings of different nano wires interconnect the different nano wires. 10. The method of claim 8 , further comprising controlling a density of the array of nano wires upon the substrate to control a density of the first plurality of pores and the second plurality of pores of the microporous structure. 11. The method of claim 8 , further comprising controlling a density of the array of nano wires upon the substrate to control a size of the first plurality of pores and the second plurality of pores of the microporous structure. 12. The method of claim 8 further comprising filling the first plurality of pores and the second plurality of pores with an active phase to form a supported liquid membrane. 13. The method of claim 8 , wherein the coating is applied while the nano wires are in a bent state. 14. The method of claim 8 , wherein the forming of the array of nano wires comprises nano imprinting the nano wires. 15. The method of claim 8 , wherein the array of nano wires formed upon the substrate is formed in a first and second two-dimensional array of columnar nano wires.
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