Methods and kits for detection of coenzyme Q10

Patent metadata

Field	Value
Publication number	US-11209422-B2
Application number	US-201816138786-A
Country	US
Kind code	B2
Filing date	Sep 21, 2018
Priority date	Mar 2, 2012
Publication date	Dec 28, 2021
Grant date	Dec 28, 2021

Abstract

The invention provides methods for rapid and quantitative extraction and detection of coenzyme Q10 in a sample readily adaptable to high throughput screening methods. The invention further provides reagents and kits for practicing the methods of the invention.

First claim

What is claimed is:

1. An isotopically labeled analog of the oxidized form of coenzyme Q10 CoQ10-d6 of the following formula:

2. An isotopically labeled analog of the reduced form of coenzyme Q10 CoQ10H 2 -d6 of the following formula:

3. A mixture for analysis by LC/MS/MS comprising a biological sample and the isotopically labeled analog of claim 1 .

4. The mixture of claim 3 , wherein said biological sample is a bodily fluid.

5. The mixture of claim 4 , wherein said bodily fluid is blood.

6. The mixture of claim 3 , wherein said biological sample is serum.

7. The mixture of claim 3 , wherein said biological sample is plasma.

8. The mixture of claim 3 , wherein said biological sample is a human sample.

9. A mixture for analysis by LC/MS/MS comprising a biological sample and the isotopically labeled analog of claim 2 .

10. The mixture of claim 9 , wherein said biological sample is a bodily fluid.

11. The mixture of claim 10 , wherein said bodily fluid is blood.

12. The mixture of claim 9 , wherein said biological sample is serum.

13. The mixture of claim 9 , wherein said biological sample is plasma.

14. The mixture of claim 9 , wherein said biological sample is a human sample.

Assignees

• Berg Llc

Inventors

• Luan Shen
• Narain Niven Rajin
• Sarangarajan Rangaprasad
• Tanna Nikunj Narendra

Classifications

• H01J49/0027

  Methods for using particle spectrometers · CPC title

• G01N2560/00

  Chemical aspects of mass spectrometric analysis of biological material · CPC title

• G01N2458/15

  Non-radioactive isotope labels, e.g. for detection by mass spectrometry · CPC title

• G01N33/82 Primary

  involving vitamins {or their receptors} · CPC title

• G01N33/52 Primary

  Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper {and including single- and multilayer analytical elements (immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72)} · CPC title