Chimeric enzymes for conversion of lignin-derived chemicals
US-2018023061-A1 · Jan 25, 2018 · US
Microbial syringol utilization
US11208642B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11208642-B2 |
| Application number | US-201916684206-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 14, 2019 |
| Priority date | Nov 14, 2018 |
| Publication date | Dec 28, 2021 |
| Grant date | Dec 28, 2021 |
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Abstract
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Disclosed herein are compositions of non-naturally occurring enzymes to enable microbial syringol utilization with GcoAB.
First claim
Opening claim text (preview).
What is claimed is: 1. A non-naturally occurring GcoAB enzyme comprising the amino acid sequence of SEQ ID NO: 1 capable of the O-aryl-demethylation of syringol and the O-aryl-demethylation of guaiacol and wherein a phenylalanine residue at the active site has been changed to an amino acid selected from the group consisting of alanine, serine, histidine, valine, isoleucine, and leucine. 2. The enzyme of claim 1 wherein a phenylalanine at the active site has been changed to alanine. 3. The enzyme of claim 1 comprising a k cat for guaiacol of at least 11 sec −1 and a k cat for syringol of at least 5.9 sec −1 . 4. The enzyme of claim 1 comprising a K M for guaiacol of less than about 40 mM and a K M for syringol of less than about 10 mM. 5. The enzyme of claim 1 comprising a kcat/K M for guaiacol of greater than about 290 mM −1 sec −1 and a kcat/K M for syringol of greater than about 600 mM −1 sec −1 . 6. The enzyme of claim 1 comprising increased specific activity for guiaicol when compared to the native GcoAB enzyme. 7. The enzyme of claim 1 wherein the specific activity for syringol is greater than 0.54 μmol sec −1 μmol −1 . 8. The enzyme of claim 1 wherein the specific activity for syringol is greater than 3.9 μmol sec −1 μmol −1 . 9. The enzyme of claim 1 wherein the specific activity for syringol is greater than 5.1 μmol sec −1 μmol −1 .
Assignees
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Classifications
- C12N9/2437Primary
Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150) · CPC title
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- What does patent US11208642B2 cover?
- Disclosed herein are compositions of non-naturally occurring enzymes to enable microbial syringol utilization with GcoAB.
- Who is the assignee on this patent?
- Alliance Sustainable Energy, Univ Montana State, Univ Of Portsmouth
- What technology area does this patent fall under?
- Primary CPC classification C12N9/2437. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 28 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).