System, method, and device for forming an array of emulsions

Patent metadata

Field	Value
Publication number	US-11207685-B2
Application number	US-201815894836-A
Country	US
Kind code	B2
Filing date	Feb 12, 2018
Priority date	Feb 13, 2017
Publication date	Dec 28, 2021
Grant date	Dec 28, 2021

Abstract

Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.

First claim

We claim:

1. A device to form an array of emulsions, comprising:
an assembly of bonded layers creating an array of emulsion formation units, each emulsion formation unit including a sample reservoir configured to be loaded with sample-containing fluid, and a droplet generation site configured to receive sample-containing fluid from the sample reservoir and to generate droplets of sample-containing fluid encapsulated by carrier fluid, the layers including a well layer forming a lower portion of each sample reservoir, a channel layer located over the well layer and forming the droplet generation site of each emulsion formation unit, and a port layer located over the channel layer and forming a plurality of ports arranged in fluid communication with the array of emulsion formation units;
wherein the port layer defines a respective sample-loading port over the sample reservoir of each emulsion formation unit, a vent port for the sample reservoir, and a channel that connects the vent port to the sample-loading port, wherein the port layer includes a body having a top surface and also includes at least one protrusion projecting upwardly from the top surface and formed integrally with the body, and wherein the at least one protrusion forms an encircling rim of each sample-loading port and each vent port.

2. The device of claim 1 , further comprising:
a frame;
a plurality of discrete microfluidic modules mounted or configured to be mounted to the frame, each module including an array of emulsion formation units, each emulsion formation unit including a sample reservoir configured to be loaded with sample-containing fluid, and a droplet generation site configured to receive sample-containing fluid from the sample reservoir and to generate droplets of sample-containing fluid encapsulated by carrier fluid, wherein each module has a plurality of ports in fluid communication with the emulsion formation units of the module; and
a sealing member configured to be bonded to a top side of each module of the plurality of modules, such that the sealing member completely covers and hermetically seals the plurality of ports of each module of the plurality of modules.

3. A device to form an array of emulsions, comprising:
an assembly of layers bonded to one another, the assembly creating an array of emulsion formation units, a vacuum port, and a vacuum manifold, each emulsion formation unit including a sample reservoir configured to be loaded with sample-containing fluid and a droplet generation site configured to receive sample-containing fluid from the sample reservoir and to generate droplets of sample-containing fluid encapsulated by carrier fluid;
wherein the vacuum port is in fluid communication with an emulsion reservoir of each emulsion formation unit of the module via the vacuum manifold.

4. The device of claim 3 , wherein the assembly of layers includes a well layer forming a lower portion of each sample reservoir and a lower portion of each emulsion reservoir of the array of emulsion formation units, and wherein the assembly of layers also includes a channel layer located between the well layer and the vacuum manifold and forming the droplet generation site of each emulsion formation unit.

5. The device of claim 2 , wherein each port of the plurality of ports has a top rim formed by a ridge, wherein the port layer has a body and at least one protrusion projecting from a top surface of the body, and wherein the at least one protrusion provides the ridge for each port of the plurality of ports.

6. The device of claim 2 , wherein the frame is a rectangular frame having a pair of lateral walls facing one another, and wherein each module is attached to the frame via complementary mating features of the module and the frame.

7. The device of claim 6 , wherein each module is mounted to the frame via snap-fit attachment.

8. The device of claim 2 , wherein each sample reservoir is located under a respective sample-loading port, and wherein the sample-loading port includes a tapered region that tapers toward the sample reservoir.

9. The device of claim 8 , wherein a neck region of the sample-loading port is located between the tapered region and the sample reservoir.

10. The device of claim 2 , wherein the array of each module is a smaller array, and wherein the plurality of modules forms a larger, rectangular array of emulsion formation units that includes the smaller array of each module.

11. The device of claim 10 , wherein each sample reservoir is configured to be loaded with sample-containing fluid via a respective sample-loading port that defines a loading axis, wherein the rectangular array has the loading axes of the sample-loading ports arranged in uniformly-spaced rows and uniformly-spaced columns, and wherein the rows and the columns have the same spacing as one another.

12. The device of claim 11 , wherein the rectangular array is an 8-by-12 array.

13. The device of claim 2 , wherein each emulsion formation unit of the plurality of modules includes an emulsion reservoir to collect an emulsion including droplets of sample-containing fluid encapsulated by carrier fluid, and wherein the droplet generation site of the emulsion formation unit includes a channel junction at which a sample channel, at least one carrier fluid channel, and a droplet channel meet one another and at which droplets are generated.

14. The device of claim 2 , wherein the plurality of ports includes a plurality of sample-loading ports, at least one carrier fluid port, and at least one vacuum/pressure port.

15. The device of claim 14 , wherein the plurality of ports also includes a respective vent port for each emulsion formation unit.

16. The device of claim 2 , wherein each module includes an assembly of layers bonded together, and wherein the assembly creates each emulsion formation unit of the module.

17. A system for forming emulsions, comprising:
the device of claim 2 ; and
at least one source of vacuum/pressure configured to be operatively coupled to the device, to drive droplet generation at each droplet generation site of each module.

Assignees

• Bio Rad Laboratories

Inventors

• Cauley Iii Thomas Henry
• Dzenitis John
• Hobbs Steve
• Schiaffino Stefano

Classifications

• B01L2200/027

  for microfluidic devices · CPC title

• B01L2200/0689

  Sealing · CPC title

• B01L3/502715

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title

• B01L3/502784 Primary

  specially adapted for droplet or plug flow, e.g. digital microfluidics · CPC title

• G01N1/38

  Diluting, dispersing or mixing samples · CPC title