Method and arrangement for focusing objects in a flow

Patent metadata

Field	Value
Publication number	US-11207683-B2
Application number	US-201716322820-A
Country	US
Kind code	B2
Filing date	Jul 28, 2017
Priority date	Aug 2, 2016
Publication date	Dec 28, 2021
Grant date	Dec 28, 2021

Abstract

At least one embodiment relates to a focusing arrangement for focusing particles or cells in a flow. The arrangement includes at least one channel for guiding the flow. The channel includes (i) at least one particle confinement structure having particle flow boundaries and (ii) at least one acoustic confinement structure having acoustic field boundaries adapted for confining acoustic fields. The acoustic field boundaries may be different from the particle flow boundaries, and the at least one acoustic confinement structure may be arranged with regard to the channel to at least partially confine acoustic fields in the channel.

First claim

The invention claimed is:

1. A focusing arrangement for focusing particles or cells in a flow, the focusing arrangement comprising:
a first channel and a second channel positioned adjacent to the first channel, wherein the first channel and the second channel are configured to guide a first flow and a second flow of particles or cells, respectively, wherein each channel comprises a particle confinement structure, and wherein the particle confinement structure comprises a pair of inward-facing surfaces configured as particle flow boundaries within which the particles or cells flow and a pair of outward-facing surfaces opposite respective inward-facing surfaces; and
an acoustic confinement structure that comprises an acoustic barrier, wherein the acoustic barrier is positioned in between adjacent outward-facing surfaces of particle confinement structures of the first channel and the second channel, to thereby configure the adjacent outward-facing surfaces as acoustic field boundaries within which acoustic fields are confined.

2. The focusing arrangement of claim 1 , wherein the acoustic confinement structure further comprises a second acoustic barrier and a third acoustic barrier, wherein the second acoustic barrier and the third acoustic barrier are respectively positioned adjacent to non-adjacent outward-facing surfaces of particle confinement structures of the first channel and the second channel.

3. The focusing arrangement of claim 1 , wherein the acoustic barrier is configured to reflect a traveling acoustic wave.

4. The focusing arrangement of claim 1 , wherein the acoustic confinement structure is a first acoustic confinement structure, and wherein the channel comprises two consecutive acoustic confinement structures including the first acoustic confinement structure and a second acoustic confinement structure.

5. The focusing arrangement of claim 1 , wherein the particle confinement structure comprises a pillar array.

6. The focusing arrangement of claim 1 , wherein the particle confinement structure comprises an acoustic transparent layer.

7. The focusing arrangement of claim 1 , further comprising a transducer configured to generate an acoustic wave between the acoustic field boundaries of the acoustic confinement structure.

8. A method comprising:
moving particles within a first channel and a second channel positioned adjacent to the first channel, wherein the first channel and the second channel are configured to guide the particles, wherein each channel comprises a particle confinement structure, and wherein the particle confinement structure comprises a pair of inward-facing surfaces configured as particle flow boundaries within which the particles flow and a pair of outward-facing surfaces opposite respective inward-facing surfaces; and
focusing the particles via an acoustic confinement structure that comprises an acoustic barrier, wherein the acoustic barrier is positioned in between adjacent outward-facing surfaces of particle confinement structures of the first channel and the second channel, to thereby configure the adjacent outward-facing surfaces as acoustic field boundaries within which acoustic fields are confined.

9. The method of claim 8 , wherein the particles comprise first particles in the first channel and second particles in the second channel, and wherein the focusing comprises (i) focusing the first particles by applying a first acoustic field and (ii) focusing the second particles by applying a second acoustic field.

10. The method of claim 8 , further comprising:
moving the particles in the first channel and the second channel away from the particle flow boundaries by applying a first acoustic field,
wherein the focusing comprises focusing the particles in the channel by applying a second acoustic field.

11. The method of claim 8 , further comprising:
restricting flow of the particles in at least one of the first channel and the second channel before focusing the particles using an acoustic field.

12. The method of claim 11 , wherein the flow of the particles is restricted using a pillar array.

13. The method of claim 11 , wherein the flow of the particles is restricted using an acoustic transparent layer.

14. The method of claim 8 , wherein the first channel, the second channel, and the acoustic confine structure are housed within a device.

15. The method of claim 14 , wherein the acoustic confinement structure is configured to confine acoustic fields, at least partially, within the first channel and the second channel.

16. The method of claim 8 , wherein the particles correspond to cells or bioparticles, and wherein the method is performed by a diagnostic device configured to focus flow of the cells or the bioparticles.

17. The method of claim 8 , wherein the method is performed by an industrial inspection device configured to monitor a liquid flow comprising the particles.

18. A diagnostic device comprising:
a focusing arrangement for focusing bioparticles or cells in a flow, the focusing arrangement comprising:
a first channel and a second channel positioned adjacent to the first channel, wherein the first channel and the second channel are configured to guide a first flow and a second flow of particles or cells, respectively, wherein each channel comprises a particle confinement structure, and wherein the particle confinement structure comprises a pair of inward-facing surfaces configured as particle flow boundaries within which the particles or cells flow and a pair of outward-facing surfaces opposite respective inward-facing surfaces, and
an acoustic confinement structure that comprises an acoustic barrier, wherein the acoustic barrier is positioned in between adjacent outward-facing surfaces of particle confinement structures of the first channel and the second channel, to thereby configure the adjacent outward-facing surfaces as acoustic field boundaries within which acoustic fields are confined; and

a module configured to make a determination of one or more of a quality or quantity of the focused bioparticles or cells and provide an output based on the determination.

19. The diagnostic device of claim 18 , wherein the acoustic confinement structure further comprises a second acoustic barrier and a third acoustic barrier, wherein the second acoustic barrier and the third acoustic barrier are respectively positioned adjacent to non-adjacent outward-facing surfaces of particle confinement structures of the first channel and the second channel particle confinement structures.

Assignees

• Imec Vzw
• Univ Leuven Kath

Inventors

• Mao Shengping
• Sohn Erik
• Rottenberg Xavier
• Liu Chengxun

Classifications

• G01N29/222

  Constructional or flow details for analysing fluids (optoacoustic fluid cells G01N29/2425) · CPC title

• A61M2205/0244

  Micromachined materials, e.g. made from silicon wafers, microelectromechanical systems [MEMS] or comprising nanotechnology · CPC title

• B01L2200/0652

  Sorting or classification of particles or molecules · CPC title

• B01L2400/0439

  ultrasonic vibrations, vibrating piezo elements · CPC title

• G01N2015/142

  Acoustic or ultrasonic focussing · CPC title