Apparatus and method for fluorescence molecular tomography

Patent metadata

Field	Value
Publication number	US-11206982-B2
Application number	US-201815983163-A
Country	US
Kind code	B2
Filing date	May 18, 2018
Priority date	Jul 25, 2017
Publication date	Dec 28, 2021
Grant date	Dec 28, 2021

Abstract

An apparatus for fluorescence molecular tomography includes an exciting light source, a carrying stage, and a receiving apparatus. The carrying stage configured to carry an subject to be examined which receives the exciting light to excite fluorescence; the receiving apparatus comprising a fluorescence detector, an exciting light detector and a beam splitter, the beam splitter is configured to allow the fluorescence to exit in a first direction and the exciting light having passed through the subject to be examined to exit in a second direction, the fluorescence detector is positioned in the first direction to the beam splitter and configured to receive and transform the fluorescence into a first electrical signal. The exciting light detector is positioned in the second direction to the beam splitter and configured to receive and transform the exciting light having passed through the subject to be examined into a second electrical signal.

First claim

What is claimed is:

1. An apparatus for fluorescence molecular tomography coupled with a computer product, and the apparatus comprising:
an exciting light source configured to provide an exciting light;
a carrying stage configured to carry a subject to be examined which receives the exciting light to excite fluorescence;
a receiving apparatus comprising a fluorescence detector, an exciting light detector and a beam splitter, wherein
the beam splitter is configured to allow the fluorescence to exit in a first direction and the exciting light having passed through the subject to be examined to exit in a second direction;
the fluorescence detector is positioned in the first direction to the beam splitter and configured to receive and detect the intensity of the fluorescence; and
the exciting light detector is positioned in the second direction to the beam splitter and is configured to receive and detect the intensity of the exciting light having passed through the subject to be examined; and

the computer product comprises a processor configured to reconstruct a fluorescence distribution image based on the first and second electrical signals,
wherein the reconstruction of the fluorescence distribution image comprises generation of a 3D fluorescence distribution image;
wherein the processor is configured to generate 3D fluorescence distribution image within the subject to be examined based on the first and second electric signals, which comprises;
a 3D absorption coefficient distribution of fluorescence within the subject to be examined is obtained from

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where A is a projection matrix μ f (r) is 3D absorption coefficient distribution of fluorescence within the subject to be examined, y x is intensity of the exciting light having passed through the subject to be examined and received by the exciting light detector, y f is intensify of the fluorescence received by the fluorescence detector, ϕ x (r′,r d ) is the intensity of the exciting light at the position of r d at the body surface of the subject to be examined ϕ f (r′, r d ) is the intensity of the fluorescence at the position of r d at the body surface of the subject to be examined, η is a fluorescence quantum productivity, ϕ x (r′r d ) is a photon density of the exciting light at a position of r′, and G(r,r d ) is a Green function solution of the exciting light at the position of r d and r.

2. The apparatus for fluorescence molecular tomography according to claim 1 , wherein the exciting light source and the receiving apparatus are rotatable relatively to the subject to be examined to enable the processor to generate a 3D fluorescence distribution image.

3. The apparatus for fluorescence molecular tomography according to claim 1 , wherein the exciting light source and the receiving apparatus are fixedly positioned, while the carrying stage is rotatable to rotate the subject to be examined.

4. The apparatus for fluorescence molecular tomography according to claim 1 , wherein the carrying stage is fixedly positioned, while the exciting light source and the

Assignees

• Boe Technology Group Co Ltd

Inventors

• Wang Guohe

Classifications

• G01N2201/103

  Scanning by mechanical motion of stage · CPC title

• G01N21/4795

  spatially resolved investigating of object in scattering medium (in vivo A61B) · CPC title

• G01N21/6456

  Spatial resolved fluorescence measurements; Imaging · CPC title

• A61B5/0073

  by tomography, i.e. reconstruction of 3D images from 2D projections (A61B5/0066 takes precedence) · CPC title

• G01N21/6428

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title