Determining a degree of red blood cell deformity within a blood sample

Patent metadata

Field	Value
Publication number	US-11199690-B2
Application number	US-202016851410-A
Country	US
Kind code	B2
Filing date	Apr 17, 2020
Priority date	Sep 17, 2015
Publication date	Dec 14, 2021
Grant date	Dec 14, 2021

Abstract

Apparatus and methods are described including acquiring microscopic images of a blood sample, and identifying deformed red blood cells within the microscopic images. A degree of red blood cell deformity within the sample is determined, at least partially based upon the identified deformed red blood cells. A sample-informative parameter that is indicative of a characteristic of the blood sample as a whole is determined, at least partially based upon the degree of red blood cell deformity within the sample. Other applications are also described.

First claim

The invention claimed is:

1. A method comprising:
acquiring microscopic images of a blood sample;
identifying deformed red blood cells within the microscopic images;
determining a degree of red blood cell deformity within the sample, at least partially based upon the identified deformed red blood cells; and
determining a sample-informative parameter that is indicative of a characteristic of the blood sample as a whole, at least partially based upon the degree of red blood cell deformity within the sample,
wherein determining the sample-informative parameter at least partially based upon the degree of red blood cell deformity within the sample comprises determining an age-related parameter of the sample, at least partially based upon the degree of red blood cell deformity within the sample.

2. The method according to claim 1 , wherein identifying deformed red blood cells within the microscopic images comprises identifying echinocytes within the microscopic images.

3. The method according to claim 1 , wherein identifying deformed red blood cells within the microscopic images comprises identifying acanthocytes within the microscopic images.

4. The method according to claim 1 , wherein determining the sample-informative parameter at least partially based upon the degree of red blood cell deformity within the sample comprises determining that the sample has a pathology, at least partially based upon the degree of red blood cell deformity within the sample.

5. The method according to claim 1 , wherein determining the sample-informative parameter at least partially based upon the degree of red blood cell deformity within the sample comprises determining that the sample is infected with a pathogen, at least partially based upon the degree of red blood cell deformity within the sample.

6. The method according to claim 1 , further comprising detecting that the sample is infected with a pathogen, wherein determining the sample-informative parameter at least partially based upon the degree of red blood cell deformity within the sample comprises identifying the pathogen, at least partially based upon the degree of red blood cell deformity within the sample.

7. A method comprising:
acquiring microscopic images of a blood sample;
identifying deformed red blood cells within the microscopic images;
determining a degree of red blood cell deformity within the sample, at least partially based upon the identified deformed red blood cells; and
determining a sample-informative parameter that is indicative of a characteristic of the blood sample as a whole, at least partially based upon the degree of red blood cell deformity within the sample,
wherein determining the sample-informative parameter at least partially based upon the degree of red blood cell deformity within the sample comprises determining that the sample has undergone prolonged exposure to a solution, at least partially based upon the degree of red blood cell deformity within the sample.

8. The method according to claim 7 , wherein determining that the sample has undergone prolonged exposure to the solution at least partially based upon the degree of red blood cell deformity within the sample comprises determining that the sample has undergone prolonged exposure to a dye, at least partially based upon the degree of red blood cell deformity within the sample.

9. The method according to claim 1 , further comprising detecting that the sample is infected with a pathogen, wherein determining the age-related parameter of the sample at least partially based upon the degree of red blood cell deformity within the sample comprises estimating an age of the pathogen, at least partially based upon the degree of red blood cell deformity within the sample.

10. Apparatus for analyzing a blood sample, the apparatus comprising:
a microscope system configured to acquire one or more microscope images of the blood sample;
an output device; and
at least one computer processor configured to:
identify deformed red blood cells within the microscopic images,
determine a degree of red blood cell deformity within the sample, at least partially based upon the identified deformed red blood cells,
determine a sample-informative parameter that is indicative of a characteristic of the blood sample as a whole, by determining an age-related parameter e sample, at least partially based upon the degree of red blood cell deformity within the sample, and
generate an output upon the output device, at least partially based upon the sample-informative parameter.

11. The apparatus according to claim 10 , wherein the at least one computer processor is configured to identify the deformed red blood cells within the microscopic images by identifying echinocytes within the microscopic images.

12. The apparatus according to claim 10 , wherein the at least one computer processor is configured to identify the deformed red blood cells within the microscopic images by identifying acanthocytes within the microscopic images.

13. The apparatus according to claim 10 , wherein the at least one computer processor is configured to determine the sample-informative parameter at least partially based upon the degree of red blood cell deformity within the sample by determining that the sample has a pathology, at least partially based upon the degree of red blood cell deformity within the sample.

14. The apparatus according to claim 10 , wherein the at least one computer processor is configured to determine the sample-informative parameter at least partially based upon the degree of red blood cell deformity within the sample by determining that the sample is infected with a pathogen, at least partially based upon the degree of red blood cell deformity within the sample.

15. The apparatus according to claim 10 , wherein the at least one computer processor is further configured to detect that the sample is infected with a pathogen, and wherein the at least one computer processor is configured to determine the sample-informative parameter at least partially based upon the degree of red blood cell deformity within the sample by identifying the pathogen, at least partially based upon the degree of red blood cell deformity within the sample.

16. Apparatus for analyzing a blood sample, the apparatus comprising:
a microscope system configured to acquire one or more microscope images of the blood sample;
an output device; and
at least one computer processor configured to:
identify deformed red blood cells within the microscopic images,
determine a degree of red blood cell deformity within the sample, at least partially based upon the identified deformed red blood cells, and
determine a sample-informative parameter that is indicative of a characteristic of the blood sample as a whole, by determining that the sample has undergone prolonged exposure to a solution, at least partially based upon the degree of red blood cell deformity within the sample, and
generate an output upon the output device, at least partially based upon the sample-informative parameter.

17. The apparatus according to claim 16 , wherein the at least one computer processor is configured to determine that the sample has undergone prolonged exposure to the solution at least partially based upon the degree of red blood cell deformity within the sample by determining that the sample has undergone prolonged exposure to a dye, at least partially based upon the degree of red blood cell deformity within the sample.

18. The apparatus according to claim 10 , wherein the at least one computer processor is furt

Assignees

• S D Sight Diagnostics Ltd

Inventors

• Eshel Yochay Shlomo
• Lezmy Natalie
• Gluck Dan
• Houri Yafin Arnon
• Pollak Joseph Joel

Classifications

• G06F18/214

  Generating training patterns; Bootstrap methods, e.g. bagging or boosting · CPC title

• G06T2207/30101

  Blood vessel; Artery; Vein; Vascular · CPC title

• G06T2207/30024

  Cell structures in vitro; Tissue sections in vitro · CPC title

• G06T2207/10056

  Microscopic image · CPC title

• G02B21/367 Primary

  providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison · CPC title