Plant derived insecticidal proteins and methods for their use

Patent metadata

Field	Value
Publication number	US-11198709-B2
Application number	US-201615750520-A
Country	US
Kind code	B2
Filing date	Jul 8, 2016
Priority date	Aug 6, 2015
Publication date	Dec 14, 2021
Grant date	Dec 14, 2021

Abstract

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, Hemipteran, fungi and nematode pest populations and for producing compositions with insecticidal activity.

First claim

That which is claimed is:

1. A recombinant polynucleotide operably linked to a heterologous regulatory element wherein the recombinant polynucleotide encodes an insecticidal polypeptide comprising an amino acid sequence selected from:
a. an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 2;
b. an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 56; and
c. an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 144.

2. The recombinant polynucleotide of claim 1 , wherein the coding region for the insecticidal polypeptide is a non-genomic polynucleotide.

3. The recombinant polynucleotide of claim 1 , wherein the polynucleotide is a cDNA.

4. The recombinant polynucleotide of claim 1 , wherein the polynucleotide is a synthetic polynucleotide.

5. The recombinant polynucleotide of claim 3 , wherein the polynucleotide has codons optimized for expression in a maize plant.

6. A DNA construct comprising the polynucleotide of claim 1 .

7. A transgenic plant or plant cell comprising the DNA construct of claim 6 .

8. A fusion protein comprising the insecticidal polypeptide encoded by the recombinant polynucleotide of claim 1 .

9. A method for controlling a Coleopteran pest, comprising contacting the insect pest population with the insecticidal polypeptide encoded by the recombinant polynucleotide of claim 1 .

10. A method of inhibiting growth or killing a Coleopteran pest or pest population, comprising contacting the insect pest with a composition comprising the insecticidal polypeptide encoded by the recombinant polynucleotide of claim 1 .

Assignees

• Du Pont
• Pioneer Hi Bred Int

Inventors

• Allen Stephen M
• Barry Jennifer Kara
• Crane Virginia
• English James J
• Fengler Kevin A
• Schepers Eric
• Udranszky Ingrid

Classifications

• A01H1/00 Primary

  Processes for modifying genotypes {; Plants characterised by associated natural traits} (A01H4/00 takes precedence) · CPC title

• C12N15/8286

  for insect resistance · CPC title

• C07K14/415 Primary

  from plants · CPC title

• A01N65/00

  Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof (containing compounds of determined constitution A01N27/00 - A01N59/00) · CPC title

• A01N47/42

  containing —N=CX2 groups, e.g. isothiourea · CPC title