Cellulose synthase inhibitors and mutant plants

Patent metadata

Field	Value
Publication number	US-11193136-B2
Application number	US-201716323707-A
Country	US
Kind code	B2
Filing date	Aug 1, 2017
Priority date	Aug 9, 2016
Publication date	Dec 7, 2021
Grant date	Dec 7, 2021

Abstract

The present invention relates to specific inhibitors of the cellulose synthase subunits 1 and 3 activity in plants, useful as a herbicide. In addition, the invention relates to mutant plants which are tolerant to the identified inhibitors. Specific mutant alleles of CESA1 and CESA3 genes can be used to obtain resistance in a plant when the inhibitors are used as herbicide.

First claim

The invention claimed is:

1. A non-naturally occurring plant mutant CESA1 protein selected from the group consisting of:
(a1) the mutant CESA1 protein having mutation of valine residue to methionine residue at the amino acid sequence position 297 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: NP_194967 which corresponds to the amino acid sequence position 27 of SEQ ID NO: 3,
(a2) the mutant CESA1 protein having mutation of valine residue to methionine residue at the amino acid sequence position 301 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_003522623 which corresponds to the amino acid sequence position 27 of SEQ ID NO: 3,
(a3) the mutant CESA1 protein having mutation of valine residue to methionine residue at the amino acid sequence position 285 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_004291468.1 which corresponds to the amino acid sequence position 27 of SEQ ID NO: 3,
(a4) the mutant CESA1 protein having mutation of valine residue to methionine residue at the amino acid sequence position 441 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_002282575.2 which corresponds to the amino acid sequence position 27 of SEQ ID NO: 3,
(a5) the mutant CESA1 protein having mutation of valine residue to methionine residue at the amino acid sequence position 301 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_004245031 which corresponds to the amino acid sequence position 27 of SEQ ID NO: 3,
(a6) the mutant CESA1 protein having mutation of valine residue to methionine residue at the amino acid sequence position 293 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: NP_001104954 which corresponds to the amino acid sequence position 27 of SEQ ID NO: 3,
(a7) the mutant CESA1 protein having mutation of valine residue to methionine residue at the amino acid sequence position 294 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: NP_001054788.1 which corresponds to the amino acid sequence position 27 of SEQ ID NO: 3,
(a8) the mutant CESA1 protein having mutation of leucine residue to phenylalanine residue at the amino acid sequence position 872 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: NP_194967 which corresponds to the amino acid sequence position 62 of SEQ ID NO: 3,
(a9) the mutant CESA1 protein having mutation of leucine residue to phenylalanine residue at the amino acid sequence position 876 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_003522623 which corresponds to the amino acid sequence position 62 of SEQ ID NO: 3,
(a10) the mutant CESA1 protein having mutation of leucine residue to phenylalanine residue at the amino acid sequence position 860 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_004291468.1 which corresponds to the amino acid sequence position 62 of SEQ ID NO: 3,
(a11) the mutant CESA1 protein having mutation of leucine residue to phenylalanine residue at the amino acid sequence position 1016 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_002282575.2 which corresponds to the amino acid sequence position 62 of SEQ ID NO: 3,
(a12) the mutant CESA1 protein having mutation of leucine residue to phenylalanine residue at the amino acid sequence position 877 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_004245031 which corresponds to the amino acid sequence position 62 of SEQ ID NO: 3;
(a13) the mutant CESA1 protein having mutation of leucine residue to phenylalanine residue at the amino acid sequence position 866 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: NP_001104954 which corresponds to the amino acid sequence position 62 of SEQ ID NO: 3,
(a14) the mutant CESA1 protein having mutation of leucine residue to phenylalanine residue at the amino acid sequence position 867 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: NP_001054788.1 which corresponds to the amino acid sequence position 62 of SEQ ID NO: 3,
(a15) the mutant CESA1 protein having mutation of serine residue to asparagine residue at the amino acid sequence position 892 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: NP_194967 which corresponds to the amino acid sequence position 82 of SEQ ID NO: 3,
(a16) the mutant CESA1 protein having mutation of serine residue to asparagine residue at the amino acid sequence position 896 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_003522623 which corresponds to the amino acid sequence position 82 of SEQ ID NO: 3,
(a17) the mutant CESA1 protein having mutation of serine residue to asparagine residue at the amino acid sequence position 880 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_004291468.1 which corresponds to the amino acid sequence position 82 of SEQ ID NO: 3,
(a18) the mutant CESA1 protein having mutation of serine residue to asparagine residue at the amino acid sequence position 1036 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_002282575.2 which corresponds to the amino acid sequence position 82 of SEQ ID NO: 3,
(a19) the mutant CESA1 protein having mutation of alanine residue to valine residue at the amino acid sequence position 1018 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: NP_194967 which corresponds to the amino acid sequence position 208 of SEQ ID NO: 3,
(a20) the mutant CESA1 protein having mutation of alanine residue to valine residue at the amino acid sequence position 1022 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_003522623 which corresponds to the amino acid sequence position 208 of SEQ ID NO: 3,
(a21) the mutant CESA1 protein having mutation of alanine residue to valine residue at the amino acid sequence position 1006 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_004291468.1 which corresponds to the amino acid sequence position 208 of SEQ ID NO: 3,
(a22) the mutant CESA1 protein having mutation of alanine residue to valine residue at the amino acid sequence position 1162 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_002282575.2 which corresponds to the amino acid sequence position 208 of SEQ ID NO: 3,
(a23) the mutant CESA1 protein having mutation of alanine residue to valine residue at the amino acid sequence position 1023 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_004245031 which corresponds to the amino acid sequence position 208 of SEQ ID NO: 3,
(a24) the mutant CESA1 protein having mutation of alanine residue to threonine residue at the amino acid sequence position 1023 of the wild-type CESA1 plant protein as set forth in GenBank sequence database sequence accession number: NP_194967 which corresponds to the amino acid sequence position 213 of SEQ ID NO: 3,
(a25) the mutant CESA1 protein having mutation of alanine residue to threonine residue at the amino acid sequence position 1027 of the wild-type CESA1 plant protein as set forth in GenBank database sequence accession number: XP_003522623 which corresponds to the amino acid sequence position 213 of SEQ ID NO: 3, and
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Assignees

• Vib Vzw
• Univ Gent
• Univ

Inventors

• De Veylder Lieven
• Vanderhaeghen Rudy
• Hu Zhubing

Classifications

• C12N9/1059

  Cellulose synthases (2.4.1.12; 2.4.1.29) · CPC title

• A01N43/90

  having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system · CPC title

• C12N15/8274 Primary

  for herbicide resistance · CPC title