Fluidic device, system, method of detecting sample material and method of purifying sample material

Patent metadata

Field	Value
Publication number	US-11192106-B2
Application number	US-201816209857-A
Country	US
Kind code	B2
Filing date	Dec 4, 2018
Priority date	Jun 6, 2016
Publication date	Dec 7, 2021
Grant date	Dec 7, 2021

Abstract

A fluidic device includes a first circulation flow path and a second circulation flow path which circulate a solution containing a sample material, the first circulation flow path and the second circulation flow path share at least a part of the flow path, and at least one selected from the group consisting of a capture unit which captures the sample material, a detection unit which detects the sample material, a valve, and a pump is provided on the shared flow path.

First claim

What is claimed is:

1. A fluidic device, comprising:
a first circulation flow path having a looped shape;
a bypass flow path having ends that are connected to first and second positions of the first circulation flow path, respectively;
one first valve that is disposed between the first and second positions of the first circulation flow path;
second and third valves that are disposed at the ends of the bypass flow path, respectively;
a first introduction flow path and a first discharge flow path that are directly connected to the first circulation flow path;
a second introduction flow path and a second discharge flow path that are directly connected to the bypass flow path; and
a pump that is disposed on the first circulation flow path.

2. The fluidic device according to claim 1 ,
wherein the second and third valves are disposed in a vicinity of the first valve, and
a second circulation flow path is formed by driving the pump in a state where the first valve is closed and the second and third valves are opened.

3. The fluidic device according to claim 1 , comprising:
a capture unit that is disposed on the first circulation flow path,
wherein the capture unit captures a sample material contained in a mixed solution in which a solution introduced to the first circulation flow path and a solution introduced to the bypass flow path are mixed.

4. The fluidic device according to claim 3 , comprising:
a detection unit that is disposed on the first circulation flow path,
wherein the detection unit detects the sample material captured by the capture unit.

5. The fluidic device according to claim 4 ,
wherein the detection unit detects a sample material bound to a detection auxiliary material, and
an auxiliary material detection unit which detects the detection auxiliary material is provided on the flow path shared by the first circulation flow path and the second circulation flow path.

6. The fluidic device according to claim 1 ,
wherein the pump comprises at least three pump valves.

7. The fluidic device according to claim 1 ,
wherein each of the first circulation flow path and the second circulation flow path has two or more metering valves, and each of the metering valves is disposed so that each of sections of the first and second circulation flow paths partitioned by the metering valves has a predetermined volume.

8. The fluidic device according to claim 7 ,
wherein at least one introduction flow path and at least one discharge flow path are connected to each of the first circulation flow path and the second circulation flow path, and
the introduction flow path and the discharge flow path are formed so that an individual solution is capable of being introduced into each of the sections of the first and second circulation flow paths partitioned by the metering valves.

9. The fluidic device according to claim 8 ,
wherein at least one introduction flow path and at least one discharge flow path are connected to all of the sections of the first and second circulation flow paths partitioned by the metering valves.

10. The fluidic device according to claim 2 ,
further comprising a capture unit to capture a sample material bound to a carrier particle.

11. The fluidic device according to claim 10 ,
wherein a magnet constituted to be capable of controlling a magnetic force is capable of being disposed in a vicinity of the capture unit.

12. A system comprising
fluidic device according to claim 1 , and a control unit which controls opening and closing of a valve.

13. A method of purifying a sample material using a fluidic device which comprises:
a first circulation flow path having a looped shape;
a bypass flow path having ends that are connected to first and second positions of the first circulation flow path, respectively;
one first valve that is disposed between the first and second positions of the first circulation flow path;
second and third valves that are disposed at the ends of the bypass flow path, respectively;
a first introduction flow path and a first discharge flow path that are directly connected to the first circulation flow path;
a second introduction flow path and a second discharge flow path that are directly connected to the bypass flow path;
a pump that is disposed on the first circulation flow path; and
a capture unit that is disposed on the first circulation flow path and captures a sample material contained in a mixed solution in which a solution introduced to the first circulation flow path and a solution introduced to the bypass flow path are mixed, the method comprising:
introducing a first liquid containing the sample material into the first circulation flow path;
introducing a second liquid containing a carrier particle to be bound to the sample material into the bypass flow path;
circulating and mixing the first liquid and the second liquid in a second circulation flow path formed by closing the first valve and opening the second and third valves, and obtaining a mixed liquid; and
capturing, by the capture unit, a complex of the sample material and the carrier particle from the mixed liquid.

14. The method according to claim 13 , further comprising:
removing the mixed liquid from the second circulation flow path after the capturing by the capture unit; and
introducing a third liquid to be mixed with the complex captured by the capture unit.

15. The method according to claim 14 ,
wherein the sample material is a nucleic acid,
the carrier particle is a silica magnetic particle, and
the capture unit captures the carrier particle by a magnet.

16. The method according to claim 13 ,
wherein each of the first circulation flow path and the second circulation flow path is partitioned into a plurality of sections each having a predetermined volume by a valve, and a liquid is quantified by introducing the liquid into each of the sections.

17. The method according to claim 13 , further comprising:
detecting the sample material.

18. A fluidic device, comprising:
a circulation flow path which circulates a solution containing a sample material; and
a merging branching portion that is connected to the circulation flow path,
wherein the merging branching portion is a space which has a lower surface and an upper surface having a triangle shape, and a valve that adjusts a flow of a fluid in a flow path is provided on one of at least two apexes of the triangle.

Assignees

• Nikon Corp

Inventors

• Kobayashi Ryo
• Mitsui Keiji
• Ueno Taro
• Shiono Hirofumi

Classifications

• B01L3/502738 Primary

  characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746) · CPC title

• G01N37/00

  Details not covered by any other group of this subclass · CPC title

• B01L2300/088

  Channel loops · CPC title

• G01N35/08

  using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis · CPC title

• B03C2201/18

  Magnetic separation whereby the particles are suspended in a liquid · CPC title