Mixtures and compositions comprising Paenibacillus strains or metabolites thereof and other biopesticides

Patent metadata

Field	Value
Publication number	US-11185078-B2
Application number	US-201716076368-A
Country	US
Kind code	B2
Filing date	Feb 6, 2017
Priority date	Feb 9, 2016
Publication date	Nov 30, 2021
Grant date	Nov 30, 2021

Abstract

The present invention relates to novel mixtures comprising, as active components, at least one isolated bacterial strain, which is a member of the genus Paenibacillus, or a cell-free extract thereof or at least one metabolite thereof, and at least one other biopesticide. The present invention also relates to compositions comprising at least one of such bacterial strains, whole culture broth or a cell-free extract or a fraction thereof or at least one metabolite thereof, and at least one other biopesticide. The present invention also relates to a method of controlling or suppressing plant pathogens or preventing plant pathogen infections by applying such composition. The present invention also relates to mixtures of fusaricidins which are pesticidal metabolites produced by the abovementioned strains, and other biopesticides.

First claim

The invention claimed is:

1. A mixture, comprising as active components
1) at least one Paenibacillus strain or a culture medium thereof, a cell-free extract thereof, or at least one metabolite thereof;
wherein the at least one Paenibacillus strain is selected from the group consisting of:
a) Paenibacillus strain Lu16774 deposited with DSMZ under Accession No. DSM 26969;
b) Paenibacillus strain Lu17007 deposited with DSMZ under Accession No. DSM 26970;
c) Paenibacillus strain Lu17015 deposited with DSMZ under Accession No. DSM 26971; and
d) a Paenibacillus strain which comprises a DNA sequence exhibiting
d1) at least 99.6% nucleotide sequence identity to the DNA sequences SEQ ID NO:4 or SEQ ID NO:9; or
d2) at least 99.8% nucleotide sequence identity to the DNA sequence SEQ ID NO:14; or
d3) at least 99.9% nucleotide sequence identity to the DNA sequences SEQ ID NO:5 or SEQ ID NO:10; or
d4) at least 99.2% nucleotide sequence identity to the DNA sequence SEQ ID NO:15; or
d5) at least 99.2% nucleotide sequence identity to the DNA sequences SEQ ID NO:6 or SEQ ID NO:11; or
d6) at least 99.8% nucleotide sequence identity to the DNA sequence SEQ ID NO:16; or
d7) at least 99.8% nucleotide sequence identity to the DNA sequences SEQ ID NO:7 or SEQ ID NO:12; or
d8) at least 99.3% nucleotide sequence identity to the DNA sequence SEQ ID NO:17; or
d9) 100.0% nucleotide sequence identity to the DNA sequences SEQ ID NO:8 or SEQ ID NO:13; or
d10) at least 99.9% nucleotide sequence identity to the DNA sequence SEQ ID NO:18;

and
2) at least one biopesticide II selected from the groups L1) to L5):
L1) at least one microbial pesticide with fungicidal, bactericidal, viricidal and/or plant defense activator activity selected from: Ampelomyces quisqualis, Aspergillus flavus, Aureobasidium pullulans, Bacillus altitudinis, B. amyloliquefaciens, B. megaterium, B. mojavensis, B. mycoides, B. pumilus, B. simplex, B. solisalsi, B. subtilis, B. subtilis var. amyloliquefaciens, Candida oleophila, C. saitoana, Clavibacter michiganensis (bacteriophages), Coniothyrium minitans, Cryphonectria parasitica, Cryptococcus albidus, Dilophosphora alopecuri, Fusarium oxysporum, Clonostachys rosea f. catenulate (also named Gliocladium catenulatum ), Gliocladium roseum, Lysobacter antibioticus, L. enzymogenes, Metschnikowia fructicola, Microdochium dimerum, Microsphaeropsis ochracea, Muscodor albus, Paenibacillus alvei, Paenibacillus polymyxa, Pantoea vagans, Penicillium bilaiae, Phlebiopsis gigantea, Pseudomonas sp., Pseudomonas chloraphis, Pseudozyma flocculosa, Pichia anomala, Pythium oligandrum, Sphaerodes mycoparasitica, Streptomyces griseoviridis, S. lydicus, S. violaceusniger, Talaromyces flavus, Trichoderma asperelloides, T. asperellum, T. atroviride, T. fertile, T. gamsii, T. harmatum, T. harzianum, T. polysporum, T. stromaticum, T. virens, T. viride, Typhula phacorrhiza, Ulocladium oudemansii, Verticillium dahlia, and/or zucchini yellow mosaic virus (avirulent strain);
L2) at least one biochemical pesticide with fungicidal, bactericidal, viricidal and/or plant defense activator activity selected from: harpin protein and/or Reynoutria sachalinensis extract;
L3) at least one microbial pesticide with insecticidal, acaricidal, molluscidal and/or nematicidal activity selected from: Agrobacterium radiobacter, Bacillus cereus, B. firmus, B. thuringiensis, B. thuringiensis ssp. aizawai, B. t . ssp. israelensis, B. t . ssp. galleriae, B. t . ssp. kurstaki, B. t . ssp. tenebrionis, Beauveria bassiana, B. brongniartii, Burkholderia spp., Chromobacterium subtsugae, Cydia pomonella granulovirus (CpGV), Cryptophlebia leucotreta granulovirus (CrleGV), Flavobacterium spp., Helicoverpa armigera nucleopolyhedrovirus (HearNPV), Helicoverpa zea nucleopolyhedrovirus (HzNPV), Helicoverpa zea single capsid nucleopolyhedrovirus (HzSNPV), Heterorhabditis bacteriophora, Isaria fumosorosea, Lecanicillium longisporum, L. muscarium, Metarhizium anisopliae, Metarhizium anisopliae var. anisopliae, M. anisopliae var. acridum, Nomuraea rileyi, Paecilomyces fumosoroseus, P. lilacinus, Paenibacillus popilliae, Pasteuria spp., P. nishizawae, P. penetrans, P. ramosa, P. thornea, P. usgae, Pseudomonas fluorescens, Spodoptera littoralis nucleopolyhedrovirus (SpliNPV), Steinernema carpocapsae, S. feltiae, S. kraussei, Streptomyces galbus , and/or S. microflavus;
L4) at least one biochemical pesticide with insecticidal, acaricidal, molluscidal, pheromone and/or nematicidal activity selected from: L-carvone, citral, (E,Z)-7,9-dodecadien-1-yl acetate, ethyl formate, (E,Z)-2,4-ethyl decadienoate (pear ester), (Z,Z,E)-7,11,13-hexadecatrienal, heptyl butyrate, isopropyl myristate, lavanulyl senecioate, cis-jasmone, 2-methyl 1-butanol, methyl eugenol, cis-jasmone, methyl jasmonate, (E,Z)-2,13-octadecadien-1-ol, (E,Z)-2,13-octadecadien-1-ol acetate, (E,Z)-3,13-octadecadien-1-ol, R-1-octen-3-ol, pentatermanone, (E,Z,Z)-3,8,11-tetradecatrienyl acetate, (Z,E)-9,12-tetradecadien-1-yl acetate, Z-7-tetradecen-2-one, Z-9-tetradecen-1-yl acetate, Z-11-tetradecenal, Z-11-tetradecen-1-01, extract of Chenopodium ambrosiodes, Neem oil, and/or Quillay extract;
L5) at least one microbial pesticide with plant stress reducing, plant growth regulator, plant growth promoting and/or yield enhancing activity selected from: Azospirillum amazonense, A. brasilense, A. lipoferum, A. irakense, A. halopraeferens, Bradyrhizobium spp., B. elkanii, B. japonicum, B. liaoningense, B. lupini, Delftia acidovorans, Glomus intraradices, Mesorhizobium spp., Rhizobium leguminosarum by. phaseoli, R. I . bv. trifolii, R. I . bv. viciae, R. tropici , and/or Sinorhizobium meliloti,

wherein the mixture comprises component 1) and component 2) in a synergistically effective amount.

2. The mixture of claim 1 , wherein said at least one Paenibacillus strain has antifungal activity against at least two of the plant pathogens selected from the group consisting of Alternaria spp., Botrytis cinerea, Phytophthora infestans and Sclerotinia sclerotiorum.

3. The mixture of claim 1 , wherein said at least one Paenibacillus strain is capable of producing at least one of the following compounds:

in a growth medium comprising at least one source of carbon and one source of nitrogen.

4. The mixture of claim 1 , wherein the component 1) comprises a substantially purified culture of said at leastone Paenibacillus strain as defined in claim 1 .

5. The mixture of claim 1 , wherein the component 1) comprises a whole culture broth, a culture medium or a cell-free extract of said at least comprises a whole culture broth, a culture medium or a cell-free extract of at least one Paenibacillus strain as defined in claim 1 .

6. The mixture of claim 1 , wherein the component 1) comprises said at least one metabolite of the at least one Paenibacillus strain selected from the group consisting of polymyxins, octapeptins, polypeptins, pelgipeptins and fusaricidins.

7. The mixture of claim 6 , wherein the at least one metabolite is selected from fusaricidins of formula I

wherein
R is selected from the group consisting of 15-guanidino-3-hydroxypentadecanoic acid (GHPD) and 12-guanidinododecanoic acid (12-GDA);
X 1 is threonine;
X 2 is selected from the group consisting of isoleucine and valine;
X 3 is selected from the group consisting of tyrosine, valine, isoleucine and phenylalanine;
X 4 is threonine;
X 5 is selected from the group consisting of

Assignees

• Basf Se

Inventors

• Siepe Isabella
• Jabs Thorsten
• Liebmann Burghard
• Schuster Annette

Classifications

• Y02A50/30

  Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change · CPC title

• A01N47/44 Primary

  Guanidine; Derivatives thereof · CPC title

• A01N63/25

  Paenibacillus · CPC title

• C12R2001/01

  Bacteria or Actinomycetales {; using bacteria or Actinomycetales} · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title