Flow cell array and uses thereof

Patent metadata

Field	Value
Publication number	US-11179697-B2
Application number	US-201715833359-A
Country	US
Kind code	B2
Filing date	Dec 6, 2017
Priority date	Feb 3, 2014
Publication date	Nov 23, 2021
Grant date	Nov 23, 2021

Abstract

Systems, computer program products, and methods for using a flow cell array are provided herein. A computer program product includes a computer readable storage medium having program instructions embodied therewith, the program instructions executable by a device to cause the device to deliver multiple items of chemical matter independently to multiple reaction sites of a flow cell array across multiple distinct instances of time; image multiple parallel chemical reactions at the multiple reaction sites of the flow cell array; and record an emission from each of the multiple chemical reactions site.

First claim

What is claimed is:

1. A method comprising:
determining, within a pixel array, locations for multiple reaction sites in multiple surface channels, wherein the multiple surface channels are aligned to a first direction, and wherein each of the pixels within the pixel array is of a size of approximately one micrometer;
forming a connection between each of the multiple channels and respective pairs of sub-surface channels by a respective pair of vias, wherein the sub-surface channels are aligned to a second direction;
wherein a designation is established wherein each of the pairs of sub-surface channels is designated for use with (i) a distinct one of multiple chemical matters, (ii) one or more of the multiple reaction site openings designated for use with the distinct one of the multiple chemical matters, and (iii) one or more of the multiple surface channels connected to the one or more reaction site openings designated for use with the distinct one of the multiple chemical matters; and
delivering, within one of the pixels within the pixel array, the multiple chemical matters to the multiple reaction sites in accordance with the established designation;
imaging multiple parallel chemical reactions at the multiple reaction sites of the pixel array; and
recording an emission from each of the multiple chemical reactions site.

2. The method of claim 1 , wherein the multiple surface channels comprise multiple microfluidic surface channels.

3. The method of claim 1 , wherein the sub-surface channels comprise microfluidic sub-surface channels.

4. The method of claim 1 , wherein the vias comprise fluidic vias.

Assignees

• IBM

Inventors

• Colgan Evan
• Lvov Alexey Y
• Polonsky Stanislav

Classifications

• B01J2219/00576

  fluorophore · CPC title

• B01L2200/0684

  Venting, avoiding backpressure, avoid gas bubbles · CPC title

• B01L3/5027

  by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip · CPC title

• B01J2219/00689

  using computers · CPC title

• B01J19/0046 Primary

  Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays (synthesis methods per se C40B50/00) · CPC title