Enzyme processing of soybeans into intact oil bodies, protein bodies, and hydrolyzed carbohydrates

Patent metadata

Field	Value
Publication number	US-11174501-B2
Application number	US-201916715617-A
Country	US
Kind code	B2
Filing date	Dec 16, 2019
Priority date	Dec 14, 2018
Publication date	Nov 16, 2021
Grant date	Nov 16, 2021

Abstract

A method of enzyme-based processing of plant-based materials includes steps of providing a bulk amount of a plant-based material having a substantial amount of the cell walls in a physically-intact condition; providing an enzyme broth having an enzyme capable of breaking down the cell walls in the physically-intact condition; combining the bulk amount of the plant-based material with the enzyme broth; allowing the enzyme capable of breaking down the cell walls in the physically-intact condition to break down at least a portion of the cell walls in the physically-intact condition to thereby produce intact oil bodies, hydrolyzed carbohydrates, and intact protein bodies; collecting a first product including the intact oil bodies; collecting a second product including the hydrolyzed carbohydrates; and collecting a third product including the intact protein bodies.

First claim

What is claimed is:

1. A method of enzyme-based processing of plant-based materials, the method comprising steps of:
providing a bulk amount of a plant-based material, the bulk amount of the plant-based material including a plurality of individual plant-based materials having cell walls, wherein the bulk amount of the plant-based material includes at least 90% of the cell walls in a physically-intact condition relative to a naturally-occurring amount of the cell walls in the physically-intact condition, wherein the physically-intact condition is defined as having unbroken cell walls;
providing an enzyme broth having an enzyme capable of breaking down the cell walls in the physically-intact condition;
combining the bulk amount of the plant-based material with the enzyme broth;
allowing the enzyme capable of breaking down the cell walls in the physically-intact condition to break down at least a portion of the cell walls in the physically-intact condition to thereby produce intact oil bodies, hydrolyzed carbohydrates, and intact protein bodies;
collecting a first product stream including the intact oil bodies;
collecting a second product stream including the hydrolyzed carbohydrates; and
collecting a third product stream including the intact protein bodies, wherein the third product stream is a solid product.

2. The method of claim 1 , wherein the bulk amount of the plant-based material includes at least 95% of the cell walls in the physically-intact condition relative to the naturally-occurring amount of the cell walls in the physically-intact condition.

3. The method of claim 1 , wherein the first product stream includes at least 50 wt. % of the intact oil bodies, wherein the second product stream includes at least 50 wt. % of the hydrolyzed carbohydrates, and wherein the third product stream includes at least 40 wt. % of the intact protein bodies.

4. The method of claim 1 , wherein the enzyme capable of breaking down the cell walls in the physically-intact condition comprises cellulase, xylanase, and pectinase.

5. The method of claim 1 , wherein the plant-based material has an average particle size from about 2.38 mm to about 1.19 mm.

6. The method of claim 1 , wherein the plant-based material has an average particle size from about 1.19 mm to about 0.42 mm.

7. The method of claim 1 , wherein the bulk amount of the plant-based material includes about 100% of the cell walls in the physically-intact condition relative to the naturally-occurring amount of the cell walls in the physically-intact condition.

8. A method of enzyme-based processing of plant-based materials, the method comprising steps of:
providing a bulk amount of a plant-based material, the bulk amount of the plant-based material including a plurality of individual plant-based materials having cell walls, wherein the bulk amount of the plant-based material includes at least 90% of the cell walls in a physically-intact condition relative to a naturally-occurring amount of the cell walls in the physically-intact condition, where the physically-intact condition is defined as having unbroken cell walls;
providing an enzyme broth having an enzyme capable of breaking down the cell walls in the physically-intact condition;
combining the bulk amount of the plant-based material with the enzyme broth;
allowing the enzyme capable of breaking down the cell walls in the physically-intact condition to break down at least a portion of the cell walls in the physically-intact condition to thereby produce intact oil bodies, hydrolyzed carbohydrates, and intact protein bodies;
collecting a first product stream including the intact oil bodies;
collecting a second product stream including the hydrolyzed carbohydrates; and
collecting a third product stream including the intact protein bodies;
wherein the method further comprises a step of sonicating the plant-based material before the step of allowing.

9. The method of claim 8 , wherein the method further comprises a step of sonicating during the step of allowing.

10. The method of claim 1 , wherein the bulk amount of the plant-based material includes a total amount of intact oil bodies, wherein the step of collecting the first product stream includes collecting at least 40 wt. % of the total amount of intact oil bodies.

11. The method of claim 1 , wherein the bulk amount of the plant-based material includes a total amount of carbohydrates, wherein the step of collecting the second product stream includes collecting at least 50 wt. % of the total amount of carbohydrates as hydrolyzed carbohydrates.

12. The method of claim 1 , wherein the amount of the plant-based material includes a total amount of intact protein bodies, wherein the step of collecting the third product stream includes collecting at least 40 wt. % of the total amount of intact protein bodies.

13. The method of claim 1 , wherein the method further comprises a step of sonicating the plant-based material before the step of allowing.

14. The method of claim 8 , wherein the third product stream is a solid product.

Assignees

• Ju Lu Kwang
• Univ Akron

Inventors

• Ju Lu-Kwang

Classifications

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• C12P7/64 Primary

  Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats · CPC title

• C12P21/00 Primary

  Preparation of peptides or proteins (single cell protein C12N1/00) · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title