Mad nucleases

Patent metadata

Field	Value
Publication number	US-11174471-B2
Application number	US-202117200089-A
Country	US
Kind code	B2
Filing date	Mar 12, 2021
Priority date	Dec 10, 2019
Publication date	Nov 16, 2021
Grant date	Nov 16, 2021

Abstract

The present disclosure provides new RNA-guided nucleases for making rational, direct edits to nucleic acids in live cells.

First claim

We claim:

1. A nuclease system capable of performing nucleic acid-guided nuclease editing, wherein the nuclease system is selected from a nuclease system comprising SEQ ID NO: 9, SEQ ID NO: 38 and SEQ ID NO: 39; a nuclease system comprising SEQ ID NO: 9, SEQ ID NO: 38 and SEQ ID NO: 40; and a nuclease system comprising SEQ ID NO: 9, SEQ ID NO: 41 and SEQ ID NO: 42.

2. The nuclease system of claim 1 comprising SEQ ID NO: 9, SEQ ID NO: 38 and SEQ ID NO: 39.

3. The nuclease system of claim 1 comprising SEQ ID NO: 9, SEQ ID NO: 38 and SEQ ID NO: 40.

4. The nuclease system of claim 1 comprising SEQ ID NO: 9, SEQ ID NO: 41 and SEQ ID NO: 42.

5. A nickase having an amino acid sequence selected from SEQ ID NO: 54 and SEQ ID NO: 55.

6. A dead nuclease having the amino acid sequence of SEQ ID NO: 56.

Assignees

• Inscripta Inc

Inventors

• Mijts Benjamin
• Kim Juhan
• Mir Aamir
• Garst Andrew
• Seamon Kyle

Classifications

• C12N15/102

  Mutagenizing nucleic acids · CPC title

• C12N9/22 Primary

  Ribonucleases {[RNase]; Deoxyribonucleases [DNase]} · CPC title

• C12N2310/20

  involving clustered regularly interspaced short palindromic repeats [CRISPR] · CPC title

• C12N15/113

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title

• C12N2800/80

  Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites · CPC title