Anti-RNA virus composition comprising EPRS protein or fragment thereof

Patent metadata

Field	Value
Publication number	US-11173192-B2
Application number	US-201916283121-A
Country	US
Kind code	B2
Filing date	Feb 22, 2019
Priority date	Aug 23, 2016
Publication date	Nov 16, 2021
Grant date	Nov 16, 2021

Abstract

The present invention relates to an EPRS (glutamyl-prolyl-tRNA synthetase) protein or a fragment thereof.The EPRS protein of the present invention or fragment thereof may bind to PCBP2 protein to activate the MAVS signaling pathway, and thus it has anti-RNA viral effects, thereby being effective for preventing or treating a RNA viral infectious disease.

First claim

What is claimed is:

1. A method of treating an RNA viral infectious disease in a subject in need thereof, comprising administering to the subject in need thereof a fragment of an EPRS (glutamyl-prolyl-tRNA synthetase) protein that is optionally bound to a drug delivery vehicle, wherein the fragment of the EPRS protein consists of an amino acid sequence selected from the group consisting of the amino acid sequence at positions 166 to 506, the amino acid sequence at positions 168 to 506, the amino acid sequence at positions 166 to 269, the amino acid sequence at positions 168 to 269, the amino acid sequence at positions 166 to 196, the amino acid sequence at positions 168 to 196, the amino acid sequence at positions 166 to 186, and the amino acid sequence at positions 168 to 186 in SEQ ID NO: 63.

2. The method of claim 1 , wherein the fragment of the EPRS protein consists of the amino acid sequence at positions 168 to 186 in SEQ ID NO: 63.

3. The method of claim 1 , wherein the RNA viral infectious disease agent is one or more selected from the group consisting of Amalgaviridae, Birnaviridae, Chrysoviridae, Cystoviridae, Endornaviridae, Hypoviridae, Megabirnaviridae, Partitiviridae, Picobirnaviridae, Reoviridae, Totiviridae, Quadriviridae, Arteriviridae, Coronaviridae, Mesoniviridae, Roniviridae, Dicistroviridae, Iflaviridae, Marnaviridae, Picornaviridae, Secoviridae, Alphaflexiviridae, Betaflexiviridae, Gammaflexiviridae, Tymoviridae, Bornaviridae, Filoviridae, Paramyxoviridae, Rhabdoviridae, Nyamiviridae, Caliciviridae, Flaviviridae, Luteoviridae, Togaviridae, Pneumoviridae, Arenaviridae, Deltavirus, and Orthomyxoviridae viruses.

4. The method of claim 1 , wherein the fragment of the EPRS protein is bound to the drug delivery vehicle.

5. The method of claim 4 , wherein the drug delivery vehicle is selected from the group consisting of a labeling material, a targeting ligand, a cell-penetrating peptide, a polymeric nanoparticle, a viral vector, a virus-like particle, and an inorganic nanoparticle.

6. The method of claim 5 , wherein the cell-penetrating peptide is selected from the group consisting of Tat, Antennapedia, Transportan, VP22, Hph-1, R11 (SEQ ID NO: 71), and R9 (SEQ ID NO: 72).

7. A method of inhibiting MAVS protein degradation in a subject in need thereof, comprising administering to the subject in need thereof a fragment of an EPRS (glutamyl-proplyl-tRNA synthetase) protein, wherein the fragment of the EPRS protein consists of an amino acid sequence selected from the group consisting of the amino acid sequence at positions 166 to 506, the amino acid sequence at positions 168 to 506, the amino acid sequence at positions 166 to 269, the amino acid sequence at positions 168 to 269, the amino acid sequence at positions 166 to 196, the amino acid sequence at positions 168 to 196, the amino acid sequence at positions 166 to 186, and the amino acid sequence at positions 168 to 186 in SEQ ID NO: 63.

8. A fragment of an EPRS protein bound to a drug delivery vehicle, wherein the fragment of the EPRS protein consists of an amino acid sequence selected from the group consisting of the amino acid sequence at positions 166 to 506, the amino acid sequence at positions 168 to 506, the amino acid sequence at positions 166 to 269, the amino acid sequence at positions 168 to 269, the amino acid sequence at positions 166 to 196, the amino acid sequence at positions 168 to 196, the amino acid sequence at positions 166 to 186, and the amino acid sequence at positions 168 to 186 in SEQ ID NO: 63.

9. The fragment of the EPRS protein bound to a drug delivery vehicle of claim 8 , wherein the fragment of the EPRS protein consists of the amino acid sequence at positions 168 to 186 of SEQ ID NO: 63.

10. The fragment of the EPRS protein bound to a drug delivery vehicle of claim 8 , wherein the drug delivery vehicle is bound to the fragment of the EPRS protein via a linker.

11. The fragment of the EPRS protein bound to a drug delivery vehicle of claim 8 , wherein the drug delivery vehicle is selected from the group consisting of a labeling material, a targeting ligand, a cell-penetrating peptide, a polymeric nanoparticle, a viral vector, a virus-like particle, and an inorganic nanoparticle.

12. The fragment of the EPRS protein of claim 11 , wherein the cell-penetrating peptide is selected from the group consisting of Tat, Antennapedia, Transportan, VP22, Hph-1, R11 (SEQ ID NO: 71), and R9 (SEQ ID NO: 72).

13. A polynucleotide encoding the fragment of the EPRS protein bound to a drug delivery vehicle of claim 8 .

14. A vector comprising the polynucleotide of claim 13 .

15. A transformant comprising the polynucleotide of claim 13 .

16. A composition comprising the fragment of the EPRS protein bound to a drug delivery vehicle of claim 8 .

17. The composition of claim 16 , wherein the composition further comprises a non-naturally occurring carrier.

18. The method according to claim 1 , wherein the subject is diagnosed with an RNA viral infectious disease.

19. The method of claim 1 , wherein the fragment of the EPRS protein that is optionally bound to a drug delivery vehicle is Tat-Epep (SEQ ID NO: 64).

Assignees

• Korea Res Inst Bioscience & Biotechnology
• Medicinal Bioconvergence Res Ct

Inventors

• Kim Myung Hee
• Lee Eun Young
• Kim Sung Hoon
• Lee Chul Ho

Classifications

• Y02A50/30

  Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change · CPC title

• A61K38/53 Primary

  Ligases (6) · CPC title

• C12Y601/01015

  Proline--tRNA ligase (6.1.1.15) · CPC title

• C07K2319/01

  containing a localisation/targetting motif · CPC title

• C12N9/93 Primary

  Ligases (6) · CPC title