Patterning silica islands onto thermoplastic shrink film

Patent metadata

Field	Value
Publication number	US-11169149-B2
Application number	US-201514967145-A
Country	US
Kind code	B2
Filing date	Dec 11, 2015
Priority date	Dec 11, 2014
Publication date	Nov 9, 2021
Grant date	Nov 9, 2021

Abstract

The disclosure relates to methods and devices for measuring samples, such as biological samples, especially those at low abundance, with high sensitivity and at low cost. A sample is disposed on a shrinkable scaffold and the shrinkable scaffold is shrunk, reducing the area where the sample is distributed, so as to effectively concentrate the sample on the surface of the scaffold. In the event that a biological sample is covalently attached to a scaffold having a silica structure, the increase in signal enhancement is also due to optical effects stemming from covalent linkage of the biological sample onto the silica structure of the scaffold. Silica (SiO2) may be deposited onto a surface of a polymer film by functionalizing the surface of the polymer film to bind silica from a sol-gel solution, and coating the film with a sol-gel solution containing silica precursors, wherein solid silica from the sol-gel solution is deposited onto the surface of the polymer film. Also disclosed is an immunoassay platform comprising a silica-encapsulated first detection agent deposited on a polymer substrate.

First claim

What is claimed is:

1. A method of detecting a biological agent deposited on a surface of a polyolefin polymer film comprising:
applying a shadow mask to the polyolefin polymer film to define blocked regions and exposed regions of the polyolefin polymer film;
functionalizing the surface of the polyolefin polymer film with oxygen plasma treatment to introduce hydroxyl groups at the exposed regions but not at the blocked regions of the polyolefin polymer film;
removing the shadow mask from the polyolefin polymer film following functionalization of the surface;
coating the functionalized polyolefin polymer film with a sol-gel solution containing the biological agent and silica precursors suspended or dissolved in a liquid;
activating the silica precursors by adding an acid or a base to the sol-gel solution, wherein the activated silica precursors react with the hydroxyl groups of the polyolefin polymer film and with each other to form a solid SiO 2 network encapsulating the biological agent, wherein the solid SiO 2 network is deposited as solid SiO 2 encapsulating the biological agent onto the surface of the polyolefin polymer film only to the regions that were exposed to the functionalizing treatment;
heat-shrinking the polyolefin polymer film; and
detecting the biological agent.

2. The method of claim 1 , wherein the biological agent is an analyte, a protein or a nucleic acid.

3. A method of detecting a biological agent deposited on a surface of a polyolefin polymer film comprising:
applying a shadow mask to the polyolefin polymer film to define blocked regions and exposed regions of the polyolefin polymer film;
functionalizing the surface of the polyolefin polymer film with oxygen plasma treatment to introduce hydroxyl groups at the exposed regions but not at the blocked regions of the polyolefin polymer film;
removing the shadow mask from the polyolefin polymer film following functionalization of the surface;
coating the functionalized polyolefin polymer film with a sol-gel solution containing silica precursors suspended or dissolved in a liquid; and
activating the silica precursors by adding an acid or a base to the sol-gel solution, wherein the activated silica precursors react with the hydroxyl groups of the polyolefin polymer film and with each other to form a solid SiO 2 network, wherein the solid SiO 2 network is deposited as solid SiO 2 onto the surface of the polyolefin polymer film only to the regions that were exposed to the functionalizing treatment;
binding a biological agent to silica already deposited onto the surface of the polyolefin polymer film;
heat-shrinking the polyolefin polymer film; and
detecting the biological agent.

4. The method of claim 3 , wherein the biological agent is an analyte, a protein or a nucleic acid.

5. A method of making an immunoassay platform comprising a silica-encapsulated first detection agent deposited on a polyolefin polymer substrate, the method comprising:
using a shadow mask to define blocked regions and exposed regions of the polyolefin polymer substrate;
functionalizing a surface of the polyolefin polymer substrate with oxygen plasma treatment to introduce hydroxyl groups at the exposed regions but not at the blocked regions of the polyolefin polymer substrate;
removing the shadow mask from the polyolefin polymer substrate following functionalization of the surface;
coating the functionalized polyolefin polymer substrate with a sol-gel solution containing silica precursors and the first detecting agent;
activating the silica precursors by adding an acid or a base to the sol-gel solution, wherein the activated silica precursors react with the hydroxyl groups of the polyolefin polymer substrate and with each other to form a solid SiO 2 network, wherein solid SiO 2 , encapsulating the first detecting agent, is deposited onto the surface of the polyolefin polymer substrate only to the regions that were exposed to the functionalizing treatment; and
heat-shrinking the polyolefin polymer substrate.

Assignees

• Univ California

Inventors

• Lin Sophia
• Khine Michelle

Classifications

• G01N33/54353 Primary

  with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393) · CPC title

• C23C18/06

  Coating on selected surface areas, e.g. using masks · CPC title

• G01N33/552

  Glass or silica · CPC title

• C23C18/04

  Pretreatment of the material to be coated (C23C18/06 takes precedence) · CPC title

• C23C18/1233

  Organic substrates · CPC title