R8 Phytophthora resistance gene in potato

Patent metadata

Field	Value
Publication number	US-11168335-B2
Application number	US-201916245767-A
Country	US
Kind code	B2
Filing date	Jan 11, 2019
Priority date	Sep 17, 2014
Publication date	Nov 9, 2021
Grant date	Nov 9, 2021

Abstract

A R8 phytophthora resistance gene from potato is described that produces a protein capable of inferring oomycete resistance, such as resistance to Phytophthora infestans when expressed in a plant. Also described is a method for providing at least partial resistance or increasing resistance in a plant against an oomycete infection comprising providing a plant or a part thereof with a nucleotide sequence of this gene or a functional fragment thereof, for example wherein said plant is a plant from the Solanaceae family, more preferably Solanum tuberosum.

First claim

The invention claimed is:

1. A method for providing at least partial resistance or increasing resistance in a plant against Phytophthora infestans infection, comprising providing a plant or a part thereof with
a. an isolated nucleic acid encoding a resistance protein that is able to confer resistance to Phytophthora infestans when expressed in a plant, wherein said isolated nucleic acid is a transgene and
i. comprises the nucleic acid sequence of SEQ ID NO: 36,
ii. encodes a protein having the amino acid sequence of SEQ ID NO: 37, or
iii. comprises a nucleic acid sequence that codes for a protein that has an identity of at least 95% with said protein having the amino acid sequence of SEQ ID NO: 37

wherein resistance is increased compared to a plant without said nucleic acid; and
b. one or more further Phytophthora infestans resistance genes selected from Rpi-edn2, Rpivnt1.1 and Rpi-blb2.

2. The method according to claim 1 , wherein said plant or part thereof is also provided with a nucleic acid sequence encoding a further Phytophthora infestans resistance gene selected from:
c. the R-genes of S. berthaultii, S. bulbocastanum, S. caripense, S. demissum, S. mkrodontum, S. mochiquense, S. papita, S. paucissectum, S. phureja, S. pinnatisectum, S. stoloniferum, S. venturii, S. vernei , or hybrids with S. tuberosum ; and/or
d. Rpi-ber, Rpi-ber1, Rpi-ber2, RB/Rpi-blb1, Rpi-blb3, R1, R2, R3, R3a, R3b, R5, R6, R7, R8, R9, R10, R11, Rpi-mcq1, Rpi-pta1, Rpi-phu1, Rpi-pn1, Rpi-sto1, Rpi-vnt1.3, Rpi-abpt, or R2-like.

3. A method for obtaining a potato plant that is resistant against Phytophthora infestans infection by crossing a donor potato plant that has the R8 gene from Solanum demissum with a potato plant of interest and testing the offspring plant of said cross for
a. the presence of a nucleic acid, wherein said nucleic acid
i. comprises the nucleic acid sequence of SEQ ID NO: 36,
ii. encodes a protein having the amino acid sequence of SEQ ID NO: 37,
iii. comprises a nucleic acid sequence that codes for a protein that has an identity of at least 95% with said protein having the amino acid sequence of SEQ ID NO: 37; and

b. the presence of one or more further Phytophthora infestans resistance genes selected from Rpi-edn2, Rpivnt1.1, and Rpi-blb2.

4. The method according to claim 3 , wherein said donor potato plant that has the R8 gene is of the variety ‘Sarpo Mira’.

5. A method for breeding a Phytophthora infestans resistant tetraploid offspring plant, said method comprising
a. doubling the ploidy level of the gametes of a diploid donor plant that already contains the nucleic acid sequence and the one or more further Phytophthora infestans resistance genes as defined in claim 3 ;
b. using said gametes in a cross with gametes of a tetraploid plant; and
c. selecting the offspring of said cross for the presence of said nucleic acid sequence.

6. The method according to claim 3 , wherein said Phytophthora infestans resistant offspring plant further comprises one or more further resistance genes selected from:
a. the R-genes of S. berthaultii, S. bulbocastanum, S. caripense, S. demissum, S. microdontum, S. mochiquense, S. papita, S. paucissectum, S. phureja, S. pinnatisectum, S. stoloniferum, S. venturii, S. vernei , or hybrids with S. tuberosum ; and/or
b. Rpi-ber, Rpi-ber1, Rpi-ber2, RB/Rpi-blb1, Rpi-blb3, R1, R2, R3, R3a, R3b, R5, R6, R7, R8, R9, R10, R11, Rpi-mcq1, Rpi-pta1, Rpi-phu1, Rpi-pnt1, Rpi-sto1, Rpi-vnt1.3, Rpi-abpt, or R2-like.

7. A method for selecting a plant or plant material or progeny thereof for its susceptibility or resistance to Phytophthora infestans infection, said method comprising the steps of testing at least part of said plant or plant material or progeny thereof for the presence or absence of the nucleic acid and the one or more further Phytophthora infestans resistance genes as defined in claim 3 .

8. The method according to claim 3 , wherein said testing involves detecting the presence of at least part of the nucleic acid sequence as shown in SEQ ID NO. 36 and is performed with a primer or a probe that specifically binds to said nucleic acid sequence.

9. A transgenic or tetraploid cell comprising the nucleic acid and one or more further Phytophthora infestans resistance genes as defined in claim 3 as a transgene.

10. A vector comprising the nucleic acid and one or more further Phytophthora infestans resistance genes as defined in claim 3 .

11. The vector according to claim 10 , further comprising a promoter and/or terminator with which the nucleic acid sequence is naturally associated.

12. A transgenic or tetraploid host cell comprising the nucleic acid and one or more further Phytophthora infestans resistance genes as defined in claim 3 as a transgene or a vector according to claim 10 .

13. A transgenic or tetraploid plant cell comprising the nucleic acid and one or more further Phytophthora infestans resistance genes as defined in claim 3 as a transgene or the vector according to claim 10 .

14. A transgenic or tetraploid plant comprising the cell according to claim 13 .

15. A plant part taken from the plant according to claim 14 .

16. The method according to claim 1 , wherein said plant or part thereof or said offspring plant further comprises or is provided with one or more further Phytophthora infestans resistance genes selected from Rpi-chc1, Bpi-ber, Rpi-sto1, and Rpi-blb3.

17. The method according to claim 3 , wherein said plant or part thereof or said offspring plant further comprises or is provided with one or more further Phytophthora infestans resistance genes selected from Rpi-chc1, Bpi-ber, Rpi-sto1, and Rpi-blb3.

18. The plant of claim 14 , wherein said plant or part thereof or said offspring plant further comprises or is provided with one or more further Phytophthora infestans resistance genes selected from Rpi-chc1, Rpi-ber, Rpi-sto1, and Rpi-blb3.

Assignees

• Univ Wageningen

Inventors

• Vossen Jacobus Hubertus
• Jo Kwang Ryong
• Visser Richard Gerardus Franciscus

Classifications

• C07K14/415 Primary

  from plants · CPC title

• C07K16/16

  against material from plants · CPC title

• A01H5/06

  Roots · CPC title

• C12N15/8282

  for fungal resistance · CPC title

• C12N15/8279 Primary

  for biotic stress resistance, pathogen resistance, disease resistance · CPC title