Culture bag, and culture apparatus

Patent metadata

Field	Value
Publication number	US-11168293-B2
Application number	US-201815982170-A
Country	US
Kind code	B2
Filing date	May 17, 2018
Priority date	May 24, 2017
Publication date	Nov 9, 2021
Grant date	Nov 9, 2021

Abstract

Provided is a culture bag accommodating a culture fluid, the culture bag is capable of suppressing foaming in the culture fluid when oscillating the culture fluid to perform a culture, and performing a culture with high efficiency. The culture bag includes a culture space accommodating a culture fluid, the culture space being an endless space to allow the culture fluid to circulate therein, in which the culture bag has an inner surface that comes into contact with the culture fluid to be accommodated therein, the inner surface including, at least in part thereof, a first surface formed of a fine structure and a second surface formed of a structure different from that of the first structure.

First claim

The invention claimed is:

1. A culture bag comprising a culture space accommodating a culture fluid, wherein:
the culture bag has an inner surface that comes into contact with the culture fluid to be accommodated therein, the inner surface including, at least in part thereof, a first surface formed of a fine structure and a second surface formed of a structure different from that of the first surface,
wherein the fine structure of the first surface has a first concave or convex pattern with a height h1 of 5 μm or more and 50 μm or less, a width w1 of 5 μm or more and 40 μm or less, and an aspect ratio h1/w1 of 0.5 or more and 10 or less, and
wherein the structure of the second surface has a second concave or convex pattern with a height h2 of 5 μm or more and 50 μm or less, a width w2 of 5 μm or more and 40 μm or less, and an aspect ratio h2/w2 of 0.5 or more and 10 or less.

2. The culture bag according to claim 1 , wherein the first concave or convex pattern has a pitch p1 of 200 nm or more and less than 1 mm.

3. The culture bag according to claim 1 , wherein the second concave or convex pattern has a pitch p2 of 200 nm or more and less than 1 mm.

4. The culture bag according to claim 1 , wherein more than one of the first surfaces and more than one of the second surfaces are alternately adjacent to each other.

5. The culture bag according to claim 1 , wherein the culture space allows the culture fluid to circulate therein.

6. The culture bag according to claim 1 , wherein the culture space has a donut shape.

7. The culture bag according to claim 6 , wherein the doughnut shape has an oval shape, or
a shape defined by one or more arcs and one or more line segments in a cross-sectional area of the culture space, wherein
the cross-sectional area is taken along a line perpendicular to a circulating direction of the culture fluid.

8. The culture bag according to claim 6 , wherein the doughnut shape has an oval shape, or
a shape defined by one or more arcs and one or more line segments formed by a main circulation path of the culture fluid.

9. The culture bag according to claim 6 , further comprising:
a plurality of first surfaces and a plurality of second surfaces, wherein
the first surfaces and the second surfaces are arranged alternately adjacent to each other while forming a plurality of rows of adjacent concentric circles, to thereby form a checkered pattern.

10. The culture bag according to claim 6 , wherein the first surface forms a plurality of concentric circles, the second surface forms a plurality of concentric circles, and wherein the concentric circles formed by the first surface and the concentric circles formed by the second surface are arranged alternately adjacent to each other.

11. The culture bag according to claim 10 , wherein the concentric circles formed by the first surface and the concentric circles formed by the second surface are intermittently continuous in the circumferential direction of the doughnut shape.

12. The culture bag according to claim 10 , wherein the concentric circles formed by the first surface are different from one another in width in the radial direction of the doughnut shape, and
wherein the concentric circles formed by the second surface are different from one another in width in the radial direction of the doughnut shape.

13. A culture apparatus comprising the culture bag according to claim 1 .

14. The culture bag according to claim 1 , wherein the culture space has a rectangular shape.

15. The culture bag according to claim 1 ,
wherein the height h1 is different from the height h2, the width w1 is different from the width w2, and the pitch p1 is different from the pitch p2.

16. The culture bag according to claim 1 , wherein the aspect ratio h1/w1 is 0.7 or more and 10 or less.

17. The culture bag according to claim 1 , wherein the aspect ratio h1/w1 is 0.9 or more and 10 or less.

18. The culture bag according to claim 1 , wherein the aspect ratio h2/w2 is 0.7 or more and 10 or less.

19. The culture bag according to claim 1 , wherein the aspect ratio h2/w2 is 0.9 or more and 10 or less.

Assignees

• Dexerials Corp

Inventors

• Odagiri Hirokazu
• Kudo Yasuyuki
• Gupta Rishabh
• Honjo Keiji

Classifications

• B01F35/512

  characterised by surface properties, e.g. coated or rough · CPC title

• B01F35/513

  Flexible receptacles, e.g. bags supported by rigid containers · CPC title

• B01F31/23

  by pivoting the containers about an axis · CPC title

• B01F2101/44

  Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation · CPC title

• C12M23/02

  Form or structure of the vessel (large containers B65D88/00) · CPC title