Methods of use of seed-origin endophyte populations

Patent metadata

Field	Value
Publication number	US-11166465-B2
Application number	US-201916657929-A
Country	US
Kind code	B2
Filing date	Oct 18, 2019
Priority date	Jun 26, 2013
Publication date	Nov 9, 2021
Grant date	Nov 9, 2021

Abstract

This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that include novel seed-origin bacterial endophytes, and synthetic combinations of seeds and/or seedlings (e.g., cereal seeds and/or seedlings) with heterologous seed-derived bacterial endophytes.

First claim

What is claimed is:

1. A method of treating a cereal agricultural plant, comprising mechanically or manually contacting a cereal agricultural plant with an agricultural formulation comprising an agriculturally acceptable carrier and a purified bacterial population that comprises a seed bacterial endophyte of the genus Enterobacter that is heterologous to a seed of the cereal agricultural plant, and has a 16S nucleic acid sequence that is at least 99% identical to a 16S nucleic acid sequence selected from the group consisting of SEQ ID NO: 541, 595, 540, and 550, and is capable of producing an auxin or acetoin, wherein the endophyte is heterologously disposed to the cereal agricultural plant in an amount effective to colonize the contacted cereal plant, wherein the contacted cereal plant has increased growth as compared to a reference cereal plant grown under the same conditions.

2. The method of claim 1 , wherein the seed bacterial endophyte is obtainable from an interior seed compartment.

3. The method of claim 1 , wherein the seed bacterial endophyte is obtainable from an exterior surface of a seed.

4. The method of claim 1 , wherein the seed bacterial endophyte is obtainable from a different cultivar, variety or crop as compared to the seed of the cereal agricultural plant.

5. The method of claim 1 , wherein the seed bacterial endophyte colonizes the roots of the contacted cereal plant.

6. The method of claim 1 , wherein the seed bacterial population includes two or more bacterial endophytes.

7. The method of claim 1 , wherein the seed bacterial endophyte is obtained from rice seed, maize seed, wheat seed, or barley seed.

8. The method of claim 1 , wherein the agricultural formulation further comprises a purified fungal population.

9. The method of claim 1 , wherein the increased growth occurs under conditions of biotic stress.

10. The method of claim 9 , wherein the biotic stress is selected from the group consisting of nematode stress, insect herbivory stress, fungal pathogen stress, bacterial pathogen stress, and viral pathogen stress.

11. The method of claim 1 , wherein the contacted cereal plant has a larger amount of lateral roots and root-hairs as compared to the reference cereal plant.

12. The method of claim 1 , wherein the contacted cereal plant has increased shoot length as compared to the reference cereal plant.

13. The method of claim 1 , wherein the contacted cereal plant has increased seedling weight as compared to the reference cereal plant.

14. The method of claim 1 , wherein the contacted cereal plant has increased shoot biomass as compared to the reference cereal plant.

15. The method of claim 1 , wherein the contacted cereal plant has increased nitrogen use efficiency as compared to the reference cereal plant.

16. The method of claim 1 , wherein the contacted cereal plant has increased root biomass as compared to the reference cereal plant.

17. The method of claim 1 , wherein the seed bacterial endophyte of the genus Enterobacter has a 16S nucleic acid sequence that is 100% identical to the 16S nucleic acid sequence selected from the group consisting of SEQ ID NO: 541, 595, 540, and 550.

18. The method of claim 1 , wherein the effective amount is at least 10{circumflex over ( )}2 CFU/ml in a liquid formulation or 10{circumflex over ( )}3 CFU/g in a non-liquid formulation.

19. The method of claim 1 , wherein the cereal agricultural plant is a seedling.

20. The method of claim 1 , wherein the method of contacting the cereal agricultural plant with the formulation is selected from the group consisting of spraying, immersing, coating, dusting, encapsulating, and dipping.

Assignees

• Indigo Ag Inc

Inventors

• Von Maltzahn Geoffrey
• Flavell Richard Bailey
• Toledo Gerardo V
• Djonovic Slavica
• Marquez Luis Miguel
• Johnston David Morris
• Millet Yves Alain
• Lyford Jeffrey
• Naydich Alexander
• Sadowski Craig

Classifications

• A01N63/20 Primary

  Bacteria; Substances produced thereby or obtained therefrom · CPC title

• A01C1/00 Primary

  Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting · CPC title

• A01C1/06 Primary

  Coating or dressing seed · CPC title

• A01N25/00

  Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application {, e.g. seed treatment or sequential application}; Substances for reducing the noxious effect of the active ingredients to organisms other than pests · CPC title

• G01N33/5097

  involving plant cells (immunoassays of plant cells G01N33/56961) · CPC title