Additive channels

Patent metadata

Field	Value
Publication number	US-11150255-B2
Application number	US-201715648182-A
Country	US
Kind code	B2
Filing date	Jul 12, 2017
Priority date	Jul 12, 2016
Publication date	Oct 19, 2021
Grant date	Oct 19, 2021

Abstract

Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it “on-chip” before moving into the experimental region of the chip.

First claim

The invention claimed is:

1. A system comprising:
a) a microfluidic device comprising:
an input channel;
an output channel;
a test channel disposed in a solid substrate, wherein said test channel comprises an input portion in fluidic communication with said input channel and an output portion in fluidic communication with said output channel;
endothelial cells disposed within at least one portion of said test channel; and
an output additive channel, wherein said output additive channel is in fluidic communication with said output portion of said test channel;

b) an input channel reservoir in fluidic communication with said input channel;
c) an output additive channel reservoir in fluidic communication with said output additive channel, said output additive channel reservoir comprising one or more reagents that inactivate a native coagulation cascade of blood; and
d) a pressure source adapted to apply pressure to both said input channel reservoir and said output additive channel reservoir.

2. The system of claim 1 , wherein said output additive channel is configured to provide a fluidic resistance that is proportional to the fluidic resistance of said input channel.

3. The system of claim 1 , wherein said output additive channel comprises a first fluidic resistor, and input channel comprises a second fluidic resistor.

4. The system of claim 1 , wherein said endothelial cells are living cells.

5. The system of claim 4 , wherein said living cells form a lumen.

6. A system comprising:
a) a microfluidic device comprising:
an input channel;
an output channel;
a test channel, wherein said test channel comprises an input portion in fluidic communication with said input channel and an output portion in fluidic communication with said output channel;
endothelial cells disposed within at least one portion of said test channel; and
an output additive channel, wherein said output additive channel is in fluidic communication with said output portion of said test channel;

b) an input channel reservoir in fluidic communication with said input channel;
c) an output additive channel reservoir in fluidic communication with said output additive channel, said output additive channel reservoir and comprising one or more reagents that inactivate a native coagulation cascade of blood and is integrated on the microfluidic device; and
d) a pressure source adapted to apply pressure to both said input channel reservoir and said output additive channel reservoir.

7. The system of claim 6 , wherein said output additive channel is configured to provide a fluidic resistance that is proportional to the fluidic resistance of said input channel.

8. The system of claim 6 , wherein said output additive channel comprises a first fluidic resistor, and input channel comprises a second fluidic resistor.

9. The system of claim 6 , wherein said endothelial cells are living cells and said living endothelial cells form a lumen.

10. A system comprising:
a) a microfluidic device comprising:
an input channel;
an output channel;
a test channel, wherein said test channel comprises an input portion in fluidic communication with said input channel and an output portion in fluidic communication with said output channel;
endothelial cells disposed within at least one portion of said test channel; and
an output additive channel, wherein said output additive channel is in fluidic communication with said output portion of said test channel;

b) an input channel reservoir in fluidic communication with said input channel;
c) an output additive channel reservoir in fluidic communication with said output additive channel, said output additive channel reservoir comprising one or more reagents that inactivate a native coagulation cascade of blood; and
d) a pressure source adapted to apply pressure to both said input channel reservoir and said output additive channel reservoir.

11. The system of claim 10 , wherein said output additive channel is configured to provide a fluidic resistance that is proportional to the fluidic resistance of said input channel.

12. The system of claim 10 , wherein said output additive channel comprises a first fluidic resistor, and input channel comprises a second fluidic resistor.

13. The system of claim 10 , wherein said endothelial cells are living cells.

14. The system of claim 13 , wherein said living cells form a lumen.

15. The system of claim 1 , said pressure source is fluidically connected in parallel with both said input channel reservoir and said output additive channel reservoir.

16. The system of claim 6 , said pressure source is fluidically connected in parallel with both said input channel reservoir and said output additive channel reservoir.

17. The system of claim 10 , said pressure source is fluidically connected in parallel with both said input channel reservoir and said output additive channel reservoir.

Assignees

• Emulate Inc

Inventors

• Levner Daniel
• Hinojosa Christopher David
• Wen Norman
• Fraser Jacob
• Nguyen Justin
• Barrile Riccardo
• Hamilton Geraldine
• Karalis Catherine
• Park Hyoungshin
• Varone Antonio
• Van Der Meer Andries
• Otieno Monicah
• Conegliano David

Classifications

• B01L2300/0867

  Multiple inlets and one sample wells, e.g. mixing, dilution · CPC title

• B01L2200/0647

  Handling flowable solids, e.g. microscopic beads, cells, particles · CPC title

• B01L2300/0887

  Laminated structure · CPC title

• B01L2300/0809

  rectangular shaped · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title