Method for serological detection of viral antigens

Patent metadata

Field	Value
Publication number	US-11150247-B2
Application number	US-201816204580-A
Country	US
Kind code	B2
Filing date	Nov 29, 2018
Priority date	May 31, 2016
Publication date	Oct 19, 2021
Grant date	Oct 19, 2021

Abstract

The present disclosure relates to a method for detecting a core polypeptide of a hepatitis C virus (HCV) in a sample from a subject involving (a) contacting said sample with a base and with a surfactant having a cationic detergent, and (b) detecting a core polypeptide of the HCV in the sample. The present invention further relates to a method for pre-processing a sample from a subject for detection of HCV, involving contacting the sample with a base and with a surfactant having a cationic detergent; and to a pre-processing reagent for detecting HCV in a sample, having a base and a surfactant including a cationic detergent, wherein the surfactant also has a nonionic detergent. Moreover, the present disclosure further relates to kits, uses, and devices related to the methods disclosed.

First claim

The invention claimed is:

1. A method for detecting a core polypeptide of a hepatitis C virus (HCV) in a sample from a subject comprising:
(a) contacting and incubating said sample with a base at a pH of at least 11.6 and with a surfactant comprising a cationic detergent, wherein said cationic detergent is a quaternary ammonium detergent, and
(b) detecting said core polypeptide of said HCV in said sample.

2. The method of claim 1 , wherein said surfactant further comprises a non-ionic detergent.

3. The method of claim 1 , wherein said method does not comprise contacting the sample with a reducing agent.

4. The method of claim 1 , wherein said method does not comprise contacting said sample with a chaotropic agent.

5. The method of claim 1 , wherein said method further comprises contacting said core polypeptide with a detector compound.

6. The method of claim 1 , wherein said detecting a core polypeptide comprises capturing at least one core polypeptide to a solid surface by means of a capture compound.

7. The method of claim 6 , wherein said capture compound and/or detector compound is a binding compound specifically binding to (i) alkaline-treated core polypeptide or to (ii) alkaline-treated core polypeptide and non-alkaline-treated core polypeptide.

8. The method of claim 1 , wherein said detecting said HCV core polypeptide comprises detecting said HCV core polypeptide in a sandwich immunoassay.

9. The method of claim 1 , wherein said base is a Bronsted-Lowry base.

10. The method of claim 1 , wherein said sample is contacted with said base and with said surfactant simultaneously.

11. The method of claim 1 , wherein said sample is a sample comprising immunoglobulins.

12. The method of claim 2 , wherein the surfactant comprises an alkyl-glycoside.

13. The method of claim 2 , wherein the surfactant comprises octylglycoside (n-octyl-.beta.-D-glucoside, CAS Number 29836-26-8).

14. The method of claim 5 wherein the detector compound is comprised of an antibody.

Assignees

• Roche Diagnostics Operations Inc

Inventors

• Bollhagen Ralf
• Upmeier Barbara
• Zarnt Toralf
• Muench Peter
• Ginter Manfred

Classifications

• G01N2469/10

  Detection of antigens from microorganism in sample from host · CPC title

• G01N33/5767 Primary

  non-A, non-B hepatitis · CPC title

• G01N2333/186

  Hepatitis C; Hepatitis NANB · CPC title

• G01N33/5306

  Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding · CPC title