Microparticle sorting method and microchip for sorting microparticles

Patent metadata

Field	Value
Publication number	US-11148173-B2
Application number	US-202016733601-A
Country	US
Kind code	B2
Filing date	Jan 3, 2020
Priority date	Aug 16, 2012
Publication date	Oct 19, 2021
Grant date	Oct 19, 2021

Abstract

There is provided a microparticle sorting method including a procedure of collecting a microparticle in a fluid that flows through a main channel in a branch channel that is in communication with the main channel by generating a negative pressure in the branch channel. In the procedure, a flow of a fluid is formed that flows toward a side of the main channel from a side of the branch channel at a communication opening between the main channel and the branch channel.

First claim

What is claimed is:

1. A microchip, comprising:
a first fluid channel through which a first fluid flows, wherein the first fluid comprises of a plurality of microparticles;
a branch channel in communication with the first fluid channel via a first communication opening;
a switching portion communicated with an actuator to generate a pressure to deflect a selected microparticle of the plurality of microparticles into the branch channel; and
a second fluid channel in communication with the branch channel via a second communication opening in the branch channel, wherein
a second fluid flows from the second fluid channel to the second communication opening, and
the second fluid channel is perpendicular to the branch channel.

2. The microchip according to claim 1 , wherein the switching portion is further configured to:
generate a force based on a change in a voltage applied to the actuator; and
change the pressure in the branch channel based on the force.

3. The microchip according to claim 2 , wherein
the actuator is configured to apply a displacement on a surface of the microchip based on the applied voltage, and
the actuator is at a position, corresponding to the branch channel, on the surface of the microchip.

4. The microchip according to claim 1 , wherein
the switching portion comprising a pressure chamber configured to communicate with the branch channel, and
the pressure chamber is configured to produce a change in volume of the branch channel.

5. The microchip according to claim 1 , wherein
the first fluid flows in a first direction, and
at least of a portion of the second fluid flows, in a second direction opposite to the first direction, through the second fluid channel from a side of the branch channel towards a side of the first fluid channel.

6. The microchip according to claim 1 , further comprising two sheath fluid channels configured to introduce sheath fluid flow into the first fluid channel to form a laminar flow.

7. The microchip according to claim 6 , further comprising a first inlet configured to communicate with the first fluid channel, a sheath inlet configured to communicate with the sheath fluid channels, a second inlet configured to communicate with the second fluid channel.

8. The microchip according to claim 7 , wherein the two sheath fluid channels comprise a sheath fluid introduction opening at a symmetrical center of the two sheath fluid channels.

9. The microchip according to claim 1 , further comprising a waste fluid channel configured to communicate with the first fluid channel via the first communication opening.

10. The microchip according to claim 1 , wherein
the actuator is on a surface of the microchip at a position corresponding to a pressure chamber, and
the pressure chamber is in the branch channel.

11. The microchip according to claim 10 , wherein
the surface of the microchip comprises a recess portion at the position corresponding to the pressure chamber, and
the actuator is in the recess portion.

12. The microchip according to claim 1 , wherein the actuator is a piezo element.

13. The microchip according to claim 1 , wherein the second fluid channel is perpendicular to a substrate layer.

14. The microchip according to claim 1 , wherein the second fluid channel is perpendicular to the first fluid channel.

15. The microchip according to claim 1 , wherein
the first fluid channel comprises a first substrate layer and a second substrate layer, and
the second fluid channel comprises a third substrate layer.

16. The microchip according to claim 1 , wherein an area of the switching portion is bigger than an area of the branch channel.

17. The microchip according to claim 1 , further comprising a processor configured to control a voltage of the actuator based on the flow of the second fluid.

18. The microchip according to claim 17 , wherein the processor is further configured to control the flow of the second fluid to prevent at least one non-target microparticle of the plurality of microparticles to enter the branch channel.

19. A microparticle sorting device, comprising
an actuator,
a microchip, comprising:
a first fluid channel through which a first fluid flows, wherein the first fluid comprises a plurality of microparticles;
a branch channel in communication with the first fluid channel via a first communication opening;
a switching portion communicated with the actuator to generate a pressure to deflect a selected microparticle of the plurality of microparticles into the branch channel; and
a second fluid channel in communication with the branch channel via a second communication opening in the branch channel, wherein
a second fluid flows from the second fluid channel to the second communication opening, and
the second fluid channel is perpendicular to the branch channel.

20. The microparticle sorting device according to claim 19 , further comprising:
a light source configured to irradiate light into the plurality of microparticles; and
a detector configured to detect light emitted from the plurality of microparticles, wherein the actuator is on a surface of the microchip.

21. The microparticle sorting device according to claim 20 , further comprising:
circuitry configured to control a voltage applied to the actuator based on the light detected by the detector to generate the pressure.

22. The microparticle sorting device according to claim 21 , wherein the pressure comprises at least one of a pulse waveform, a step waveform, or an undershoot-step waveform.

23. The microparticle sorting device according to claim 19 , wherein the plurality of microparticles are at least one of cells, chromosomes, liposomes, mitochondria, cell organelles, viruses, nucleic acids, proteins, or complexes thereof.

Assignees

• Sony Corp

Inventors

• Ito Tatsumi

Classifications

• B07C5/342 Primary

  according to optical properties, e.g. colour {(according to radiation transmittivity B07C5/3416)} · CPC title

• G01N15/1484

  microstructural devices · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title

• G01N21/85

  Investigating moving fluids or granular solids · CPC title

• G01N2015/1006

  for cytology · CPC title