Photoelectrical device for concentration detection, method for concentration detection thereof and method for testing an antibiotic susceptibility on bacteria

Patent metadata

Field	Value
Publication number	US-11143585-B2
Application number	US-201816234597-A
Country	US
Kind code	B2
Filing date	Dec 28, 2018
Priority date	Dec 28, 2018
Publication date	Oct 12, 2021
Grant date	Oct 12, 2021

Abstract

A photoelectrical device for detection of bacterial cell density includes a substrate, a driving electrode layer, an AC power source and a photoelectric conversion layer. The driving electrode layer is disposed on the substrate and includes a central electrode and a peripheral electrode pattern surrounding the central electrode. A fluid sample is disposed on the driving electrode layer. The AC power source is electrically connected to the driving electrode layer, and used to produce a non-uniform alternating electric field in the fluid sample on the driving electrode layer for driving the target bioparticles to gather up on the central electrode to form a particle cluster. The photoelectric conversion layer is used for receiving a light detecting beam after passing through the particle cluster and outputting an electric current based on the optical density of light detecting beam. The electric current changes as a concentration of the target bioparticles changes.

First claim

What is claimed is:

1. A photoelectrical device for concentration detection adapted to detect a concentration of a plurality of target bioparticles in a fluid sample, the photoelectrical device comprising:
a light source, adapted to emit a light detecting beam;
a substrate, having an upper surface;
a driving electrode layer, disposed on the upper surface of the substrate and comprising a central electrode;
an AC power source, electrically connected to the driving electrode layer; and
a photoelectric conversion element, disposed oppositely to the driving electrode layer;
wherein the substrate further has a lower surface opposite to the upper surface, the photoelectric conversion element is disposed on the lower surface of the substrate, the substrate is a transparent plate, and both the driving electrode layer and the photoelectric conversion element are transparent conductive films;
wherein the light detecting beam irradiates on the photoelectric conversion element after passing through the driving electrode layer and the substrate, the fluid sample is adapted to be disposed on the upper surface of the driving electrode layer, the AC power source is used to generate a non-uniform alternating electric field in the fluid sample on the upper surface of the driving electrode layer, the non-uniform alternating electric field drives the target bioparticles to gather up on the central electrode to form a particle cluster, the photoelectric conversion element is used to receive the light detecting beam after passing through the particle cluster, the photoelectric conversion element outputs an electric current based on the light detecting beam, and the electric current changes as a concentration of the target bioparticles changes; and
wherein the photoelectrical device further comprises a peripheral electrode located around the central electrode, and the central electrode is not in contact with the peripheral electrode.

2. The photoelectrical device according to claim 1 , further comprising a measurement electrode, wherein the measurement electrode is connected to the photoelectric conversion element and used to transmit the electric current.

3. The photoelectrical device according to claim 1 , wherein a material of the photoelectric conversion element comprises metallic oxides or silicon.

4. The photoelectrical device according to claim 1 , further comprising a transparent cover disposed oppositely to the substrate, wherein the driving electrode layer faces the transparent cover, and a detecting space is formed between the central electrode and the transparent cover.

5. The photoelectrical device according to claim 4 , further comprising a transparent electrode layer formed on the transparent cover, wherein the transparent electrode layer and the driving electrode layer face each other, and the transparent electrode layer is electrically connected to the AC power source.

6. The photoelectrical device according to claim 1 , wherein the peripheral electrode comprises:
a first ring electrode, surrounding the central electrode by using the central electrode as a center; and
a second ring electrode, surrounding the central electrode and the first ring electrode by using the central electrode as a center, wherein the first ring electrode and the second ring electrode are interdigiated and do not contact.

7. The photoelectrical device according to claim 1 , wherein the peripheral electrode comprises:
a ring electrode, surrounding the central electrode by using the central electrode as a center; and
a plurality of auxiliary electrodes, connected with the ring electrode and extending radially from the ring electrode.

Assignees

• Nat Applied Res Laboratories

Inventors

• Cheng I-Fang
• Chen Tzu-Ying
• Chen Yi-Ling

Classifications

• C12Q1/18

  Testing for antimicrobial activity of a material · CPC title

• B03C7/00

  Separating solids from solids by electrostatic effect · CPC title

• B03C5/022

  Non-uniform field separators · CPC title

• B03C5/005

  Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength · CPC title

• G01N15/0656 Primary

  using electric, e.g. electrostatic methods or magnetic methods (by investigating individual particles G01N15/1031, G01N15/12) · CPC title