O-succinyl homoserine transferase variant and method of producing O-succinyl homoserine using the same

Patent metadata

Field	Value
Publication number	US-11142779-B2
Application number	US-201816627655-A
Country	US
Kind code	B2
Filing date	Jun 29, 2018
Priority date	Jun 30, 2017
Publication date	Oct 12, 2021
Grant date	Oct 12, 2021

Abstract

Provided are an O-succinyl homoserine transferase variant, a polynucleotide encoding the variant, a microorganism comprising the variant, and a method of producing O-succinyl homoserine using the microorganism.

First claim

The invention claimed is:

1. A polypeptide having O-succinyl homoserine transferase activity, the polypeptide comprising substitution of leucine at the position corresponding to amino acid 313 of SEQ ID NO: 1 with arginine, cysteine, isoleucine, or lysine, wherein the amino acid sequence of the polypeptide has at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 1.

2. The polypeptide having O-succinyl homoserine transferase activity of claim 1 , wherein the polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 59, SEQ ID NO: 67, SEQ ID NO: 75, and SEQ ID NO: 81.

3. A polynucleotide comprising a nucleotide sequence encoding the polypeptide having O-succinyl homoserine transferase activity of claim 1 .

4. The polynucleotide of claim 3 , wherein the nucleotide sequence is selected from the group consisting of SEQ ID NO: 60, SEQ ID NO: 68, SEQ ID NO: 76, and SEQ ID NO: 82.

5. An O-succinyl homoserine-producing microorganism of the genus Corynebacterium , wherein the microorganism comprises the polypeptide having O-succinyl homoserine transferase activity of claim 1 .

6. The O-succinyl homoserine-producing microorganism of claim 5 , wherein the microorganism overexpresesses an aspartokinase.

7. The O-succinyl homoserine-producing microorganism of claim 5 , wherein the microorganism is Corynebacterium glutamicum.

8. The O-succinyl homoserine-producing microorganism of claim 5 , wherein one or more genes encoding cystathionine synthase, O-acetyl homoserine (thiol)-lyase, and homoserine kinase is inactivated.

9. The O-succinyl homoserine-producing microorganism of claim 5 , wherein the microorganism overexpresesses an aspartokinase, and one or more genes encoding cystathionine synthase, O-acetyl homoserine (thiol)-lyase, and homoserine kinase is inactivated.

10. A method of producing O-succinyl homoserine, the method comprising the steps of:
culturing the O-succinyl homoserine-producing microorganism of claim 5 in a medium to produce O-succinyl homoserine; and
isolating or collecting the O-succinyl homoserine from the microorganism or the medium.

11. A method of producing L-methionine, the method comprising the steps of:
(a) culturing the O-succinyl homoserine-producing microorganism of claim 5 in a medium to produce O-succinyl homoserine; and
(b) reacting the O-succinyl homoserine with sulfide to produce L-methionine.

12. The method of producing L-methionine of claim 11 , further comprising the step of isolating or collecting the O-succinyl homoserine from the microorganism or the medium.

13. The method of producing L-methionine of claim 11 , further comprising the step of isolating or collecting the L-methionine.

Assignees

• Cj Cheiljedang Corp

Inventors

• Kim Kyungrim
• Shim Jihyun
• Kim Hyun Ah
• Shin Yong Uk
• Lee Peter

Classifications

• C12P13/12 Primary

  Methionine; Cysteine; Cystine · CPC title

• C12N9/1029

  transferring groups other than amino-acyl groups (2.3.1) · CPC title

• C12P13/06 Primary

  Alanine; Leucine; Isoleucine; Serine; Homoserine · CPC title

• C07C319/08

  by replacement of hydroxy groups or etherified or esterified hydroxy groups · CPC title

• C12N15/77

  for Corynebacterium; for Brevibacterium · CPC title