Cell treatment container and cell treatment device

Patent metadata

Field	Value
Publication number	US-11142735-B2
Application number	US-201916712238-A
Country	US
Kind code	B2
Filing date	Dec 12, 2019
Priority date	Jan 15, 2019
Publication date	Oct 12, 2021
Grant date	Oct 12, 2021

Abstract

To provide a cell treatment container, including: a first member having a flow path in which a cell suspension including a liquid and cells dispersed in the liquid flows through, the flow path formed on a surface of the first member; a second member arranged to face the surface of the first member; and a damming formed in one or both of the first member and the second member, in which the damming is provided with a protrusion part protruding from the first member into the flow path to form a gap for allowing the liquid in the cell suspension to pass through the gap and for damming up the cells in the cell suspension, and a pillar extending from the protrusion part at a first end and being joined to the second member at a second end.

First claim

What is claimed is:

1. A cell treatment container, comprising:
a first member having a flow path in which a cell suspension including a liquid and cells dispersed in the liquid flows through, the flow path formed on a surface of the first member;
a second member arranged to face the surface of the first member; and
a damming formed in one or both of the first member and the second member, wherein
the damming is provided with:
a protrusion part protruding from the first member into the flow path to form a gap for allowing the liquid in the cell suspension to pass through the gap and for damming up the cells in the cell suspension, and
a pillar extending from the protrusion part at a first end and being joined to the second member at a second end.

2. The cell treatment container according to claim 1 , wherein
the protrusion part has a plurality of the pillars.

3. The cell treatment container according to claim 1 , wherein
the first member is made of an elastomer.

4. The cell treatment container according to claim 1 , wherein
a plurality of the flow paths are formed side by side in the first member, and
the damming is arranged in each of the flow paths.

5. The cell treatment container according to claim 1 , wherein
the second member is a glass substrate.

6. The cell treatment container according to claim 1 , wherein
the flow path includes:
a first flowing section having an inlet port for the cell suspension, and
a second flowing section being branched out from the first flowing section and having an inlet port for a liquid medium to be supplied to the damming through the first flowing section.

7. A cell treatment device, comprising:
the cell treatment container according to claim 6 ; and
a first pump connected to an inlet port for the cell suspension.

8. The cell treatment device according to claim 7 , further comprising
a second pump connected to the inlet port for the liquid medium.

9. The cell treatment device according to claim 8 , further comprising
a control device for controlling operations of the first pump and the second pump, wherein
the control device is operable to control the first pump and the second pump so that a flow volume of the cell suspension to be introduced into the first flowing section is larger than a flow volume of the liquid medium to be introduced into the second flowing section.

Assignees

• Shimadzu Corp

Inventors

• Miyake Chikara
• Kanai Masaki
• Fujiyama Yoichi
• Kuramoto Satoshi

Classifications

• C12M29/10

  Perfusion · CPC title

• C12M23/16 Primary

  Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093) · CPC title

• B01L2200/0647

  Handling flowable solids, e.g. microscopic beads, cells, particles · CPC title

• B01L2300/0681

  Filter · CPC title

• B01L2300/0861

  Configuration of multiple channels and/or chambers in a single devices · CPC title