Spot array substrate, method for producing same, and nucleic acid polymer analysis method and device

Patent metadata

Field	Value
Publication number	US-11130985-B2
Application number	US-201515529122-A
Country	US
Kind code	B2
Filing date	Oct 7, 2015
Priority date	Nov 27, 2014
Publication date	Sep 28, 2021
Grant date	Sep 28, 2021

Abstract

In order to reduce the cost of producing a spot array substrate and reduce the cost of nucleic acid polymer analysis, a spot array substrate is used which is produced by preparing a resin substrate 402 having a surface on which an uneven pattern is formed and a plurality of bead sitting positions set in a two-dimensional array within the uneven pattern, and loading surface-modified beads onto the bead sitting positions of the resin substrate.

First claim

The invention claimed is:

1. A spot array substrate comprising:
a resin substrate having a surface including an uneven pattern that includes a plurality of grooves that form rhombic patterns, and a plurality of bead sitting positions set in a two-dimensional array within the uneven pattern; and
a plurality of surface-modified beads loaded into the bead sitting positions of the resin substrate,
wherein a thermal expansion coefficient of a material of the resin substrate is in a range from 10 −5 to 10 −4 /° C.,
wherein the plurality of bead sitting positions are intersections of the plurality of grooves disposed in the resin substrate,
wherein an epoxy group is disposed as a film only on inner surfaces of the respective plurality of grooves of the resin substrate,
wherein the respective surface-modified beads loaded in the bead sitting positions are physically clamped by inner surfaces of the grooves of the bead sitting positions, which are physically contracted, and
wherein the surface-modified beads are at least one of functional group surface-modified beads and oligo DNA surface-modified beads.

2. The spot array substrate according to claim 1 , wherein the grooves have a smaller width than the dimensions of the bead sitting positions.

3. The spot array substrate according to claim 1 , wherein the respective surface-modified beads are immobilized at the respective bead sitting positions of the resin substrate by chemical bonding.

4. The spot array substrate according to claim 1 , wherein the bulk material of the surface-modified beads is a material including silicon oxide or a material including a magnetic body.

5. The spot array substrate according to claim 1 , wherein the surface-modified beads are functional group surface-modified beads.

6. The spot array substrate according to claim 1 , wherein the surface-modified beads are oligo DNA surface-modified beads.

7. The spot array substrate according to claim 1 , wherein the resin substrate contains a cycloolefin polymer or a cycloolefin copolymer as a component.

8. The spot array substrate according to claim 1 , wherein the surface density of the bead sitting positions is 6.6×10 6 /cm 2 to 180×10 6 /cm 2 .

9. The spot array substrate according to claim 1 , wherein a size of the respective surface-modified beads is equal to a size of the respective intersections of the grooves.

Assignees

• Hitachi High Tech Corp

Inventors

• Itabashi Naoshi
• Migitaka Sonoko
• Narahara Masatoshi
• Shoji Tomohiro
• Ono Yukio

Classifications

• B01J19/0046 Primary

  Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays (synthesis methods per se C40B50/00) · CPC title

• B01J2219/00659

  Two-dimensional arrays · CPC title

• B01J2219/00466

  in a slurry · CPC title

• B01J2219/00621

  by physical means, e.g. trenches, raised areas · CPC title

• B01J2219/00722

  Nucleotides · CPC title