Recovery of steviol glycosides

Patent metadata

Field	Value
Publication number	US-11117916-B2
Application number	US-202016868934-A
Country	US
Kind code	B2
Filing date	May 7, 2020
Priority date	Jul 31, 2013
Publication date	Sep 14, 2021
Grant date	Sep 14, 2021

Abstract

The present invention relates a process for the recovery of one or more steviol glycosides from a steviol glycoside-containing fermentation broth, which method comprises (a) providing a fermentation broth comprising one or more steviol glycosides and one or more non-steviol glycoside components; (b) separating the liquid phase of the broth from the solid phase of the broth; (c) providing an adsorbent resin; (d) contacting the liquid phase of the broth with the adsorbent resin in order to separate at least a portion of the one or more steviol glycosides from the non-steviol glycoside components, thereby to recover one or more steviol glycosides from the fermentation broth containing one or more steviol glycosides. The invention also relates to a purified steviol glycoside composition prepared using such a process.

First claim

The invention claimed is:

1. A process for recovering one or more steviol glycosides from a steviol glycoside-containing fermentation broth, which process comprises:
(a) providing,
(a1) a fermentation broth comprising one or more steviol glycosides, one or more non-steviol glycoside components, and a solvent; or
(a2) said fermentation broth, separated into a liquid phase and a solid phase;

(b) providing an elution solvent;
(c) providing an adsorbent resin;
(d) contacting the adsorbent resin with:
the fermentation broth; or
the liquid phase;
so that at least a portion of the one or more non-steviol glycoside components adsorbs onto the adsorbent resin,

(e) eluting the one or more steviol glycosides from the adsorbent resin with elution solvent, to yield a recovered steviol glycoside-containing solution; and
(f) optionally desorbing the one or more non-steviol glycoside components from the adsorbent resin;
wherein:
the elution solvent comprises 50% weight or less ethanol, and 50% weight or greater water; and
the adsorbent resin is a polymethacrylate resin, a functionalized polymethacrylate-divinylbenzene resin, or an amino (NH2) bonded methacrylate/divinylbenzene copolymer resin.

2. The process according to claim 1 , wherein the adsorbent resin is provided in a packed column.

3. The process of claim 1 , wherein the liquid phase of the fermentation broth is provided, and further wherein the contacting is with said liquid phase.

4. The process according to claim 3 , wherein the adsorbent resin is provided in a packed column in an expanded bed mode.

5. The process according to claim 2 , wherein the adsorbent resin is a polymethacrylate resin.

6. The process according to claim 1 , wherein the process comprises adsorb/desorb chromatography.

7. The process according to claim 3 , wherein the process comprises adsorb/desorb chromatography.

8. The process according to claim 6 , wherein the adsorbent resin is provided in a packed column.

9. The process according to claim 1 , wherein the elution solvent comprises 20% weight or less ethanol, and 80% weight or greater water.

10. The process according to claim 3 , wherein the elution solvent comprises 20% weight or less ethanol, and 80% weight or greater water.

11. The process according to claim 1 , wherein the process comprises fractionation chromatography.

12. The process according to claim 11 , wherein the fractionation chromatography comprises:
(a) contacting the adsorbent resin with:
the liquid phase; or
the fermentation broth;
so that at least a portion of the one or more non-steviol glycoside components adsorbs onto the adsorbent resin, wherein one or more steviol glycosides propagates through the adsorbent resin at a faster rate than one or more non-steviol glycosides; and

(b) collecting a steviol glycoside-containing solution from the adsorbent.

13. The process according to claim 12 , wherein the adsorbent resin is provided in a packed column.

14. The process according to claim 12 wherein the solvent comprises 20% weight or greater ethanol, and 80% weight or less water.

15. The process according to claim 12 , wherein the solvent comprises 25% to 35% weight ethanol, and 65% to 75% water.

16. The process according to claim 1 , wherein the adsorbent resin has a surface area of about 900 m 2 /gram or greater.

17. The process according to claim 6 , wherein the adsorbent is functionalized with tertiary amines or quaternary amines.

18. The process according to claim 1 , wherein the recovered steviol glycoside-containing solution has a purity that is at least about 10% greater as compared to a purity of the liquid phase or fermentation broth.

19. The process according to claim 1 , wherein the purified recovered steviol glycoside-containing solution comprises, on a dry solids basis, at least about 95% weight of Rebaudioside A, Rebaudioside D or Rebaudioside M.

20. The process according to claim 1 , wherein the recovered steviol glycoside-containing solution is spray-dried to provide a powder.

21. The process according to claim 2 , wherein the adsorbent resin is a functionalized polymethacrylate-divinylbenzene resin.

22. The process according to claim 2 , wherein the adsorbent resin is an amino (NH2) bonded methacrylate/divinylbenzene copolymer resin.

Assignees

• Dsm Ip Assets Bv

Inventors

• Galaev Igor

Classifications

• C07H15/24 Primary

  Condensed ring systems having three or more rings · CPC title

• C07H1/08

  from natural products · CPC title