Combined organ and hematopoietic cells for transplantation tolerance of grafts

Patent metadata

Field	Value
Publication number	US-11116794-B2
Application number	US-201916291492-A
Country	US
Kind code	B2
Filing date	Mar 4, 2019
Priority date	Feb 26, 2013
Publication date	Sep 14, 2021
Grant date	Sep 14, 2021

Abstract

Methods and compositions are provided for combined transplantation of a solid organ and hematopoietic cells to a recipient, where tolerance to the graft is established through development of a persistent mixed chimerism. An individual with persistent mixed chimerism, usually for a period of at least six months, is able to withdraw from the use of immunosuppressive drugs after a period of time sufficient to establish tolerance.

First claim

What is claimed is:

1. A cellular product for establishing mixed chimerism in a solid organ transplant recipient, the product comprising:
at least 1×10 7 CD34 + cells/kg recipient weight, wherein the cells are in a container that comprises a cryopreservation solution; and
at least 1×10 5 CD3 + cells/kg recipient weight, wherein the cells are in a container that comprises a cryopreservation solution,
wherein the cellular product is substantially devoid of platelets and red blood cells.

2. The cellular product of claim 1 , comprising at least about 1×10 6 CD3 + cells/kg recipient weight.

3. The cellular product of claim 1 , comprising at least about 1×10 7 CD34 + cells/kg recipient weight and at least about 4×10 7 CD3 + cells/kg recipient weight.

4. The cellular product of claim 1 , wherein the CD34 + cells and the CD3 + cells are provided in separate containers.

5. The cellular product of claim 1 , wherein the CD34 + cells and the CD3 + cells are provided as a mixture in a common container.

6. The cellular product of claim 1 , wherein the CD34 + cells and the CD3 + cells are HLA mismatched to an HLA type of the solid organ transplant recipient.

7. The cellular product of claim 1 , wherein CD34 + cells and the CD3 + cells are from a single apheresis product.

8. The cellular product of claim 1 , wherein the CD34 + cells have been enriched.

9. The cellular product of claim 8 , wherein the CD34 + cells have been column enriched.

10. A cellular product for establishing mixed chimerism in a solid organ transplant recipient that is HLA mismatched from the solid organ transplant donor, the product comprising:
at least 1×10 7 CD34 + solid organ transplant donor derived cells/kg recipient weight in a container that comprises a cryopreservation solution, wherein the solid organ transplant donor's CD34 + cells are of an HLA type that is mismatched to the solid organ transplant recipient's HLA type; and
greater than 4×10 7 CD3 + solid organ transplant donor derived cells/kg recipient weight, in a container that comprises a cryopreservation solution, wherein the solid organ transplant donor's CD3 + cells are of an HLA type that is mismatched to the solid organ transplant recipient's HLA type,
wherein the cellular product is substantially devoid of platelets and red blood cells.

11. The cellular product of claim 10 , wherein the CD3 + cells are from about 4×10 7 to about 1×10 8 solid organ transplant donor derived cells/kg recipient weight.

12. The cellular product of claim 10 , wherein the CD3 + cells are greater than 1×10 8 solid organ transplant donor derived cells/kg recipient weight.

13. The cellular product of claim 10 , wherein the CD34 + cells and the CD3 + cells are provided in separate containers.

14. The cellular product of claim 10 , wherein the CD34 + cells and the CD3 + cells are HLA mismatched to the solid organ transplant recipient at least one of six alleles of HLA-A, HLA-B, and HLA-DR.

15. The cellular product of claim 10 , wherein the CD34 + cells and the CD3 + cells are HLA mismatched to the solid organ transplant recipient at all six HLA alleles.

16. The cellular product of claim 10 , wherein CD34 + cells and the CD3 + cells are from a single apheresis product.

17. The cellular product of claim 10 , wherein CD34 + cells and the CD3 + cells are from multiple apheresis products.

18. The cellular product of claim 10 , wherein the solid organ is selected from the group consisting of heart, intestine, liver, lung, pancreas, and kidney.

19. The cellular product of claim 18 , wherein the solid organ is kidney.

Assignees

• Univ Leland Stanford Junior

Inventors

• Strober Samuel
• Lowsky Robert

Classifications

• A61K35/14 Primary

  Blood; Artificial blood (perfluorocarbons A61K31/02; umbilical cord blood A61K35/51; haemoglobin A61K38/42) · CPC title

• A61K40/418

  Antigens related to induction of tolerance to non-self · CPC title

• A61K40/22

  Immunosuppressive or immunotolerising · CPC title

• A61K40/11

  T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells · CPC title

• A61K40/10

  characterised by the cell type used · CPC title