Pest control methods

Patent metadata

Field	Value
Publication number	US-11116221-B2
Application number	US-201414894808-A
Country	US
Kind code	B2
Filing date	May 28, 2014
Priority date	Jun 3, 2013
Publication date	Sep 14, 2021
Grant date	Sep 14, 2021

Abstract

The technology provided herein relates to methods of multi-species insect pest control by incorporating an inhibitor against the structural sheath protein (SHP) into the body of an agricultural target pest, and to pest control agents to be used in the method and to transgenic crop, greenhouse and ornamental plants.

First claim

What is claimed is:

1. A pest control method comprising incorporating an inhibitor against a structural sheath protein (SHP) into a body of an agricultural target pest expressing the SHP, wherein the inhibitor is a compound selected from the group consisting of the following (a) or (b):
(a) an RNAi inducing compound targeted at a nucleic acid coding SHP or parts thereof; or
(b) a nucleic acid construct intracellularly producing an RNAi inducing compound targeted at a nucleic acid coding SHP or parts thereof;
wherein the RNAi inducing compound is a compound selected from the group consisting of short interfering RNA (siRNA), microRNA (miRNA), short hairpin RNAs (shRNA), double stranded RNA (dsRNA) and a precursor thereof; and
wherein the target pest belongs to Acyrthosiphon pisum and ingestion by the target pest of the inhibitor reduces reproduction of the target pest.

2. The pest control method according to claim 1 , wherein the SHP is encoded by the nucleic acid sequence of SEQ ID NO:1, or a homolog thereof, wherein the homolog has a sequence identity of at least 90% to SEQ ID NO: 1 and encodes a functional SHP in the target pest.

3. The pest control method according to claim 1 , wherein the inhibitor against SHP is incorporated into the pest by applying an agent containing the inhibitor to the plant prior to attack of the target pest.

4. The pest control method according to claim 1 , comprising incorporating the inhibitor into the body of the target pest by ingesting a transgenic plant containing a gene encoding the inhibitor.

5. The method of claim 1 , wherein the SHP is encoded by the nucleic acid sequence of SEQ ID NO:1.

6. The method of claim 1 , wherein the reproduction is reduced by about 50%.

7. The method of claim 1 , wherein ingestion by the target pest of the inhibitor results in eventual death of the target pest.

Assignees

• Fraunhofer Ges Forschung
• Univ Justus Liebig Giessen

Inventors

• Will Torsten
• Vilcinskas Andreas
• Fischer Rainer

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C07K14/43563

  from insects · CPC title

• C07K14/435 Primary

  from animals; from humans · CPC title

• A01N57/16 Primary

  containing heterocyclic radicals · CPC title

• C12N15/8286

  for insect resistance · CPC title