Isolated polynucleotides and polypeptides and methods of using same for increasing plant yield, biomass, growth rate, vigor, oil content, abiotic stress tolerance of plants and nitrogen use efficiency

Patent metadata

Field	Value
Publication number	US-11111499-B2
Application number	US-201816234643-A
Country	US
Kind code	B2
Filing date	Dec 28, 2018
Priority date	May 3, 2011
Publication date	Sep 7, 2021
Grant date	Sep 7, 2021

Abstract

Provided are isolated polynucleotides encoding a polypeptide at least 80% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs: 757, 456-756, 758-774, 8385-10836, and 10838-14462; and isolated polynucleotide comprising nucleic acid sequences at least 80% identical to SEQ ID NO: 377, 1-376, 378-455, and 775-8384. Also provided are nucleic acid constructs comprising same, isolated polypeptides encoded thereby, transgenic cells and transgenic plants comprising same and methods of using same for increasing yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, abiotic stress tolerance, and/or nitrogen use efficiency of a plant.

First claim

What is claimed is:

1. A method of increasing leaf number, growth rate of plot coverage and/or growth rate of rosette diameter of a plant, comprising:
(a) over-expressing within the plant a polypeptide comprising an amino acid sequence at least 87% identical to SEQ ID NO: 638, wherein said over-expressing of said polypeptide is effected by transforming a cell of the plant with an exogenous polynucleotide comprising a nucleic acid sequence encoding said polypeptide, and
(b) selecting said plant over-expressing said polypeptide for an increased trait selected from the group consisting of: leaf number, growth rate of plot coverage and growth rate of rosette diameter as compared to a native plant of the same species under the same growth conditions,
thereby increasing the leaf number, growth rate of plot coverage and growth rate of rosette diameter of the plant.

2. The method of claim 1 , wherein said amino acid sequence is at least 90% identical to the amino acid sequence set forth by SEQ ID NO: 638.

3. The method of claim 1 , wherein said amino acid sequence is at least 95% identical to the amino acid sequence set forth by SEQ ID NO:638.

4. The method of claim 1 , wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs: 638, 10583, 10584, 10585, and 10586.

5. The method of claim 1 , wherein said trait is leaf number.

6. The method of claim 1 , further comprising:
(a) isolating regenerable portion of said plant selected according to claim 1 so as to obtain isolated regenerable portion of said selected plants; and
(b) regenerating plants from said isolated regenerable portion of said selected plants, wherein plants without the said trait are not isolated and not regenerated.

7. The method of claim 1 , wherein said nucleic acid sequence is selected from the group consisting of SEQ ID NOs: 437, 183, and 3903-3908.

8. The method of claim 1 , wherein said selecting is for increased leaf number, increased growth rate of plot coverage and increased growth rate of rosette diameter as compared to a native plant of the same species under the same growth conditions.

9. The method of claim 1 , wherein said selecting is for increased leaf number and increased growth rate of rosette diameter as compared to a native plant of the same species under the same growth conditions.

Assignees

• Evogene Ltd

Inventors

• Matarasso Noa
• Emmanuel Eyal
• Panik David
• Dangoor Inbal Nurith
• Karchi Hagai

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12N15/8246

  Non-starch polysaccharides, e.g. cellulose, fructans, levans · CPC title

• C12N15/8247 Primary

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• C07K14/415

  from plants · CPC title