Analysis unit for a transportable microfluidic device, in particular for sample preparation and molecule analysis

Patent metadata

Field	Value
Publication number	US-11110457-B2
Application number	US-201816221152-A
Country	US
Kind code	B2
Filing date	Dec 14, 2018
Priority date	Dec 28, 2017
Publication date	Sep 7, 2021
Grant date	Sep 7, 2021

Abstract

An analysis unit formed by an analysis body housing an analysis chamber and having a sample inlet and a supply channel configured to fluidically connect the sample inlet to the analysis chamber. Dried assay reagents are arranged in the analysis chamber and are contained in an alveolar mass. For instance, the alveolar mass is a lyophilized mass formed by excipients and by assay-specific reagents.

First claim

The invention claimed is:

1. A portable microfluidic device, comprising:
a cartridge including a first layer, a second layer and a body between the first layer and the second layer, the body having a first side coupled to the first layer, a second side opposite the first side coupled to the second layer and a third side between the first side and the second side having a sample inlet opening, the cartridge including:
an extraction chamber in fluidic connection with the sample inlet opening, the extraction chamber defined by a first recess in the first side of the body and the first layer;
a waste chamber in fluidic connection with the extraction chamber, the waste chamber defined by the first layer, a through opening in the body and a corresponding recess in the second layer;
a collector extending along the extraction chamber and the waste chamber, the collector including an analysis chamber defined by a second recess in the first side of the body and a heating and temperature-control element coupled to the second recess, wherein the heating and temperature-control element is coupled to the first side of the body via a through opening in the first layer; and
a fluidic circuit that couples the extraction chamber, the collector and the waste chamber; and
dried assay reagents in the analysis chamber, wherein the dried assay reagents are part of an alveolar mass.

2. The portable microfluidic device according to claim 1 , wherein the analysis chamber is a first reaction chamber and the collector comprises a plurality of further reaction chambers in fluidic connection with each other through a supply channel, each further reaction chamber accommodating a respective further alveolar mass including respective dried assay reagents.

3. A method for sample analysis, comprising:
filling a supply channel of a portable microfluidic device with a sample to be analyzed, the portable microfluidic device including:
a cartridge including a first layer, a second layer and a body between the first layer and the second layer, the body having a first side coupled to the first layer, a second side opposite the first side coupled to the second layer and a third side between the first side and the second side having a sample inlet opening, the cartridge including:
an extraction chamber in fluidic connection with the sample inlet opening, the extraction chamber defined by a first recess in the first side of the body and the first layer; and
a waste chamber in fluidic connection with the extraction chamber, the waste chamber defined by the first layer, a through opening in the body and a corresponding recess in the second layer;
a collector extending along the extraction chamber, the collector including an analysis chamber, wherein the supply channel that couples the extraction chamber and the collector, the analysis chamber defined by a second recess in the first side of the body and a heating and temperature-control element coupled to the second recess, wherein the heating and temperature-control element is coupled to the first side of the body via a through opening in the first layer; and
dried assay reagents in the analysis chamber, wherein the dried assay reagents are part of an alveolar mass; and

causing the alveolar mass to absorb a predetermined amount of the sample to be analyzed in the analysis chamber.

4. The method according to claim 3 , wherein the sample to be analyzed fills the supply channel by capillarity.

5. The method according to claim 3 , comprising, after causing the alveolar mass to absorb, applying an emptying force to the supply channel.

6. The method according to claim 5 , comprising, after applying the emptying force, introducing a sealing mineral oil or liquid paraffin.

7. The method according to claim 5 , comprising, after applying the emptying force, heating and melting walls of the analysis chamber to provide a seal.

8. The portable microfluidic device according to claim 2 , wherein the supply channel is configured to enable movement of liquid samples by capillarity.

9. The portable microfluidic device according to claim 2 , wherein the collector further comprise a sample chamber in fluidic connection with the supply channel.

10. The portable microfluidic device according to claim 1 , wherein the body further comprises a forth side opposite the third side and having a fluidic inlet in fluidic communication with the extraction chamber and a fluidic outlet in fluidic communication with the waste chamber.

11. The portable microfluidic device according to claim 10 , wherein the fluidic circuit comprises a first pneumatic channel that fluidically couples the extraction chamber to the waste chamber, a reagent discharge channel that fluidically couples the extraction chamber to the waste chamber, a product transfer channel that fluidically couples the extraction chamber to the analysis chamber, and a second pneumatic channel that fluidically couples the analysis chamber to the fluidic outlet.

12. The portable microfluidic device according to claim 11 , wherein a first valve is coupled to the first pneumatic channel, a second valve is coupled to the reagent discharge channel and a third valve is coupled to the second pneumatic channel.

13. The portable microfluidic device according to claim 12 , wherein the fluidic inlet and fluidic outlet are coupled to a control machine through a first connection element and a second connection element, respectively.

14. The portable microfluidic device according to claim 13 , wherein the control machine comprises a pump coupled to the fluidic outlet and adapted to generate a suction pressure within the cartridge.

15. The portable microfluidic device according to claim 13 , wherein the control machine comprises an actuator group.

16. The portable microfluidic device according to claim 15 , wherein the actuator group comprises one or more magnetic-valve actuators adapted to control the first valve, the second valve and the third valve.

17. The portable microfluidic device according to claim 13 , wherein the control machine comprises an optical-detection unit adapted to detect reactions in the analysis chamber.

18. The portable microfluidic device according to claim 10 , further comprising a plurality of reagent chambers coupled to the fluidic inlet, the plurality of reagent chambers adapted to provide preparation reagents to the extraction chamber.

19. A portable microfluidic device, comprising:
a casing having a sample inlet opening and housing:
an extraction chamber in fluidic connection with the sample inlet opening;
a waste chamber in fluidic connection with the extraction chamber;
a collector extending along the extraction chamber and the waste chamber, the collector including an analysis unit, the analysis unit comprising:
a plurality of analysis chambers arranged in an array;
dried assay reagents in each of the plurality of analysis chambers, wherein the dried assay reagents in each of the plurality of analysis chambers are part of an alveolar mass; and
a supply channel configured to fluidically couple the plurality of analysis chambers to the extraction chamber and the waste chamber, the supply channel having a first branch and a second branch extending in a first direction and a third branch that couples the first branch to the second branch extending in a second direction traversing the first direction, the first branch coupled to a first set of the plurality of analysis chambers and the second branch coupled to a second set of the plurality of analysis chambers; and

a fluidic cir

Assignees

• St Microelectronics Srl

Inventors

• Cereda Marco
• Raia Lillo
• Pirola Danilo

Classifications

• B01J19/0093 Primary

  Microreactors, e.g. miniaturised or microfabricated reactors (laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027) · CPC title

• B01F33/452

  using independent floating stirring elements · CPC title

• B01F33/30

  Micromixers · CPC title

• B01F33/406

  in receptacles with gas supply only at the bottom (B01F33/405 takes precedence) · CPC title

• A61B5/150732

  Needle holders, for instance for holding the needle by the hub, used for example with double-ended needle and pre-evacuated tube · CPC title