Bead packing in microfluidic channels

Patent metadata

Field	Value
Publication number	US-11110456-B2
Application number	US-201616099246-A
Country	US
Kind code	B2
Filing date	Jul 12, 2016
Priority date	Jul 12, 2016
Publication date	Sep 7, 2021
Grant date	Sep 7, 2021

Abstract

A microfluidic bead-packing method includes activating a first micropump to transfer active microbeads through an inlet microchannel from a bead suspension reservoir to an adsorbing channel; packing the microbeads in the adsorbing channel; and activating a second micropump to reverse flow through at least a portion of the inlet microchannel and to transfer a sample fluid through the inlet microchannel from a sample reservoir to the adsorbing channel such that the sample fluid interacts with the packed microbeads.

First claim

What is claimed is:

1. A method comprising:
for each of a plurality of parallel microfluidic bead-packing devices:
activating a first micropump to transfer active microbeads through an inlet microchannel from a bead suspension reservoir to an adsorbing channel;
packing the microbeads in the adsorbing channel; and
activating a second micropump to reverse flow through at least a portion of the inlet microchannel and to transfer a sample fluid through the inlet microchannel from a sample reservoir to the adsorbing channel such that the sample fluid interacts with the packed microbeads, the first micropump and the second micropump being integrated within the inlet microchannel;

wherein the bead suspension reservoir and the sample reservoir are shared among and connected to each of the plurality of parallel microfluidic bead-packing devices.

2. The method of claim 1 , further comprising quantifying flow through the adsorbing channel.

3. The method of claim 2 , wherein the activating the second micropump is based on determining that the quantified flow through the adsorbing channel is less than a predetermined threshold.

4. The method of claim 3 , wherein the flow through the adsorbing channel is measured with a flow meter.

5. The method of claim 1 , further comprising activating a microfluidic mixer located in the adsorbing channel.

6. The method of claim 1 , further comprising activating an outlet nozzle to drain the sample fluid from the adsorbing channel.

7. The method of claim 1 , wherein a bead clogging structure is located between the adsorbing channel and an outlet microchannel to pack the microbeads in the adsorbing channel.

8. The method of claim 7 , wherein the bead clogging structure comprises a number of posts and gaps located between the adsorbing channel and the outlet microchannel to pack the microbeads in the adsorbing channel and to permit flow of the sample fluid from the adsorbing channel to the outlet microchannel.

9. A microfluidic bead-packing system comprising:
a plurality of microbead packing devices, each of the plurality of microbead packing devices including:
an adsorbing chamber to pack together a plurality of microbeads;
an inlet microchannel fluidly coupled to a bead suspension reservoir associated with a first micropump, a sample reservoir associated with a second micropump, and the adsorbing channel;
a bead clogging filter;
an outlet nozzle; and
the first micropump and the second micropump integrated within the inlet microchannel; and

the bead suspension reservoir associated with the first micropump, the bead suspension reservoir fluidly coupled to each of the plurality of the microbead packing devices;
the sample reservoir associated with the second micropump, the sample reservoir fluidly coupled to each of the plurality of the microbead packing devices; and
a control device electrically coupled to each of the first micropump and the second micropump to sequentially provide fluids from the bead suspension reservoir and the sample reservoir to the adsorbing chamber and activate each of the first and second micropumps and outlet nozzle;
wherein each of the plurality of microbead packing devices are connected in a parallel arrangement between the bead suspension reservoir and the sample reservoir, the bead suspension reservoir and the sample reservoir being shared among each of the plurality of microbead packing devices.

10. The system of claim 9 , wherein the bead clogging filter comprises a number of posts and gaps between the adsorbing chamber and the outlet nozzle.

11. The system of claim 9 , wherein each of the plurality of microbead packing devices further comprise a microfluidic mixer to mix fluids and a flow meter to measure flow through the adsorbing chamber.

12. The system of claim 11 , wherein each of the microfluidic mixer is an active mixer and is electrically coupled to and controlled by the control device.

13. A microfluidic bead-packing system comprising:
a plurality of parallel microfluidic bead-packing devices, wherein each of the plurality of microfluidic bead-packing devices include:
an adsorbing channel to pack a plurality of active-surface microbeads;
an inlet microchannel fluidly coupled to a bead suspension reservoir associated with a first micropump, a sample reservoir associated with a second micropump, and the adsorbing channel;
the first micropump and the second micropump integrated within the inlet microchannel;
an outlet microchannel fluidly coupled to the adsorbing channel and including an outlet nozzle; and
a bead clogging structure between the adsorbing channel and the outlet microchannel;
wherein the first micropump, the second micropump, and the outlet nozzle each have electrical control connections and are activated in sequence to introduce the plurality of active-surface microbeads into the adsorbing channel, introduce sample fluid into the adsorbing channel, and drain sample fluid from the adsorbing channel through the outlet microchannel via the outlet nozzle, in that order;

wherein the bead suspension reservoir and the sample reservoir are shared among and connected to each of the plurality of parallel microfluidic bead-packing devices.

14. The microfluidic bead-packing system of claim 13 , wherein each of the bead clogging structures is a filter that comprises a plurality of posts arranged to form gaps large enough to permit passage of objects of interest in the sample fluid but small enough to block passage of the plurality of active-surface microbeads.

Assignees

• Hewlett Packard Development Co

Inventors

• Kornilovich Pavel
• Govyadinov Alexander
• Markel David P
• Torniainen Erik D

Classifications

• B01L3/5025

  for parallel transport of multiple samples · CPC title

• B01L2200/143

  Quality control, feedback systems · CPC title

• B01L2300/0663

  Whole sensors · CPC title

• B01L2300/0864

  comprising only one inlet and multiple receiving wells, e.g. for separation, splitting · CPC title

• B81C1/00206

  Processes for functionalising a surface, e.g. provide the surface with specific mechanical, chemical or biological properties · CPC title