Decellularization of plant cell culture materials for tissue engineering and drug delivery

Patent metadata

Field	Value
Publication number	US-11110203-B2
Application number	US-201916548510-A
Country	US
Kind code	B2
Filing date	Aug 22, 2019
Priority date	Aug 22, 2018
Publication date	Sep 7, 2021
Grant date	Sep 7, 2021

Abstract

Provided herein are enzymatically decellularized cells, and methods of producing said cells, that can be used in a scaffold. The scaffolds featured herein are biocompatible and can comprise decellularized cells that have been modified to express a bioactive agent or molecule.

First claim

We claim:

1. A method for decellularizing cells, the method comprising:
contacting a plurality of plant cells with a composition comprising a nuclease comprising DNase, thereby decellularizing the plurality of plant cells, wherein the plurality of plant cells are cellulose producing plant cells.

2. The method of claim 1 , wherein the DNase is DNaseI.

3. The method of claim 1 further comprising culturing the plurality of plant cells prior to contacting the cells with the nuclease.

4. The method of claim 3 , further comprising isolating cellular material from the cultured plurality of plant cells.

5. A method of producing a biocompatible scaffold in vitro, the method comprising:
contacting cellulose producing cultured plant cells with a composition comprising a DNase, thereby producing decellularized cultured plant cells; and
creating a biocompatible scaffold in vitro from the decellularized [modified] cultured plant cells.

6. The method of claim 5 further comprising modifying the cellulose producing cultured plant cells to express a bioactive agent.

7. The method of claim 6 , wherein the bioactive agent is a molecule that directs mammalian cell expansion, differentiation, or a cellular response.

8. The method of claim 6 , wherein the bioactive agent is imbedded, attached, or associated with cell walls of the cellulose producing cultured plant cells.

9. The method of claim 5 , wherein the DNase is DNaseI.

10. The method of claim 5 , wherein the composition further comprises trypsin, collagenase, lipase, dispase, thermolysin, and α-galactosidase.

11. The method of claim 1 further comprising, prior to contacting, subjecting the plurality of cultured plant cells to lyophilization.

12. The method of claim 5 further comprising, prior to contacting, subjecting the cultured plant cells to lyophilization.

Assignees

• Worcester Polytech Inst

Inventors

• Coburn Jeannine
• Gaudette Glenn
• Phan Nhi

Classifications

• A61L27/54 Primary

  Biologically active materials, e.g. therapeutic substances {(A61L27/227 takes precedence)} · CPC title

• A61L27/3687

  characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents · CPC title

• A61L27/3637

  characterised by the origin of the biological material other than human or animal, e.g. plant extracts, algae · CPC title

• C12Y302/01022

  Alpha-galactosidase (3.2.1.22) · CPC title

• A61L27/3695 Primary

  characterised by the function or physical properties of the final product, where no specific conditions are defined to achieve this (A61L27/3687, A61L27/3691 take precedence) · CPC title