Laser desorption/ionization method, mass spectrometry method, sample support body, and production method for sample support body

Patent metadata

Field	Value
Publication number	US-11101124-B2
Application number	US-201816647237-A
Country	US
Kind code	B2
Filing date	Aug 6, 2018
Priority date	Sep 21, 2017
Publication date	Aug 24, 2021
Grant date	Aug 24, 2021

Abstract

A laser desorption/ionization method, includes: a first step of preparing a sample support body including a substrate on which a plurality of through holes opening to a first surface and a second surface facing each other are formed, a conductive layer provided on at least the first surface, and a matrix provided in the plurality of through holes; a second step of mounting a sample on a mounting surface of a mounting portion, and of disposing the sample support body on the sample such that the second surface is in contact with the sample; and a third step of ionizing a component of the sample that is mixed with the matrix and is moved to the first surface side from the second surface side through the through hole by irradiating the first surface with laser light while a voltage is applied to the conductive layer.

First claim

The invention claimed is:

1. A laser desorption/ionization method, comprising:
a first step of preparing a sample support body including a substrate on which a plurality of through holes opening to a first surface and a second surface facing each other are formed, a conductive layer disposed on at least the first surface, and a matrix provided in the plurality of through holes;
a second step of mounting a sample on a mounting surface of a mounting portion, and of disposing the sample support body on the sample such that the second surface is in contact with the sample; and
a third step of ionizing a component of the sample that is mixed with the matrix and is moved to the first surface side from the second surface side through the through hole by irradiating the first surface with laser light while a voltage is applied to the conductive layer, in a state in which the sample is disposed between the mounting portion and the sample support body.

2. A mass spectrometry method, comprising:
each of the steps of the laser desorption/ionization method according to claim 1 ; and
a fourth step of detecting the component that is ionized in the third step.

3. A laser desorption/ionization method, comprising:
a first step of preparing a sample support body including a substrate having conductivity on which a plurality of through holes opening to a first surface and a second surface facing each other are formed, and a matrix provided in the plurality of through holes;
a second step of mounting a sample on a mounting surface of a mounting portion, and of disposing the sample support body on the sample such that the second surface is in contact with the sample; and
a third step of ionizing a component of the sample that is mixed with the matrix and is moved to the first surface side from the second surface side through the through hole by irradiating the first surface with laser light while a voltage is applied to the substrate, in a state in which the sample is disposed between the mounting portion and the sample support body.

4. A mass spectrometry method, comprising:
each of the steps of the laser desorption/ionization method according to claim 3 ; and
a fourth step of detecting the component that is ionized in the third step.

5. A sample support body supporting a sample in a laser desorption/ionization method, the sample support body comprising:
a substrate on which a plurality of through holes opening to a first surface and a second surface facing each other are formed;
a conductive layer provided on at least the first surface; and
a matrix provided in the plurality of through holes.

6. The sample support body according to claim 5 ,
wherein the substrate is formed by performing anodic oxidation with respect to a valve metal or silicon.

7. The sample support body according to claim 5 ,
wherein a width of the through hole is 1 nm to 700 nm.

8. A sample support body supporting a sample in a laser desorption/ionization method, the sample support body comprising:
a substrate having conductivity on which a plurality of through holes opening to a first surface and a second surface facing each other are formed; and
a matrix provided in the plurality of through holes.

9. A production method for a sample support body supporting a sample in a laser desorption/ionization method, the production method comprising:
a first step of preparing a substrate on which a plurality of through holes opening to a first surface and a second surface facing each other are formed, and a conductive layer is provided on at least the first surface;
a second step of introducing a matrix solution into the plurality of through holes; and
a third step of drying the matrix solution.

10. The production method for a sample support body according to claim 9 ,
wherein in the second step, the matrix solution is dropped with respect to the plurality of through holes from the first surface side or the second surface side.

11. The production method for a sample support body according to claim 9 ,
wherein in the second step, the substrate is dipped in the matrix solution.

12. A production method for a sample support body supporting a sample in a laser desorption/ionization method, the production method comprising:
a first step of preparing a substrate having conductivity on which a plurality of through holes opening to a first surface and a second surface facing each other are formed;
a second step of introducing a matrix solution into the plurality of through holes; and
a third step of drying the matrix solution.

Assignees

• Hamamatsu Photonics Kk

Inventors

• Kotani Masahiro
• Ohmura Takayuki

Classifications

• G01N27/623

  combined with mass spectrometry · CPC title

• H01J49/164 Primary

  Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI] (sample holders H01J49/0418) · CPC title

• H01J49/26

  Mass spectrometers or separator tubes · CPC title

• H01J49/0418 Primary

  for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates · CPC title