Sample fragmentation device using heating and pressure regulation between sample injector and separation column

Patent metadata

Field	Value
Publication number	US-11101121-B2
Application number	US-201615568529-A
Country	US
Kind code	B2
Filing date	Mar 30, 2016
Priority date	May 19, 2015
Publication date	Aug 24, 2021
Grant date	Aug 24, 2021

Abstract

For increasing a speed of fragmentation of a sample, such as a protein and a peptide, to enhance introduction efficiency into a detector, such as a mass spectrometer, a liquid feeding pump 2, a sample injector 3, and a separation column 6 that are connected via pipes are included, and further, a heating unit 4 for heating a pipe between the sample injector 3 and the separation column 6, and a pressure regulating unit 5 provided between the heating unit 4 and the separation column 6 for regulating the inner pressure of the pipe heated by the heating unit 4, are included.

First claim

The invention claimed is:

1. A sample fragmentation device for providing fragmented samples for mass spectrometry, comprising
a liquid feeding pump, a sample injector, and a separation column that are connected via pipes,
a heating unit for fragmenting the liquid sample flowing through the pipe, disposed between the sample injector and the separation column,
a pressure regulating unit provided between the heating unit and the separation column for regulating the inner pressure of the pipe heated by the heating unit, and
a cooling unit disposed on a pipe for cooling the pipe heated by the heating unit,
wherein the cooling unit is disposed on and in contact with the heating unit to cool the heating unit thereby cooling the pipe, and
wherein the pressure regulating unit is provided with a flow path selector valve and a conductance regulating unit, and the flow path selector valve switches between a flow path that passes through the heating unit and is connected to the separation column and a separate flow path that does not pass through the heating unit and is connected to the separation column.

2. The sample fragmentation device according to claim 1 , wherein the pressure regulating unit is provided with a flow path selector valve and a conductance regulating unit, and the flow path selector valve switches a flow path connected to the heating unit between the separation column and the conductance regulating unit.

3. The sample fragmentation device according to claim 2 , comprising a plurality of pipes provided with the heating units, wherein the flow path selector valve sequentially switches flow paths connected to a plurality of heating units to the separation column and to the conductance regulating unit.

4. The sample fragmentation device according to claim 1 ,
wherein the cooling unit includes a jacket housing a liquid and an inlet and an outlet to circulate the liquid.

5. The sample fragmentation device according to claim 1 , wherein a Peltier device is disposed on a heat block of the heating unit and a heat sink is disposed between a fan and the Peltier device.

Assignees

• Hitachi High Tech Corp

Inventors

• Hasegawa Hideki
• Kumano Shun
• Hashimoto Yuichiro

Classifications

• G01N30/30 Primary

  of temperature · CPC title

• G01N30/32

  of pressure or speed (G01N30/36 takes precedence) · CPC title

• G01N30/88

  Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 · CPC title

• G01N27/62

  by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode · CPC title

• G01N30/26

  Conditioning of the fluid carrier; Flow patterns · CPC title