Pretreatment apparatus and pretreatment method

Patent metadata

Field	Value
Publication number	US-11099200-B2
Application number	US-201715842470-A
Country	US
Kind code	B2
Filing date	Dec 14, 2017
Priority date	Dec 15, 2016
Publication date	Aug 24, 2021
Grant date	Aug 24, 2021

Abstract

A pretreatment apparatus (100) for performing pretreatment of a sample is provided with a holder (610) for holding a container (10) containing a sample, a centrifugal separation device (600) for rotating the container (10) held by the holder (610) to perform a centrifugal separation, a temperature adjustment device (420) for adjusting the temperature of the sample in the container (10) in the holder (610), an input device (320) for receiving from a user a designated temperature that is adjusted by the temperature adjustment device (420), a controller (300) for controlling the temperature adjustment device (420) in accordance with the designated temperature received via the input device (320).

First claim

What is claimed is:

1. A pretreatment apparatus for performing pretreatment of a sample containing cells, the apparatus comprising:
a centrifugal separation device having a holder that holds a container containing the sample and configured to centrifuge the sample by rotating the container in the holder;
a sample temperature adjustment device configured to adjust a temperature of the sample in the container in the holder;
first and second reagent storages respectively configured to hold a first reagent container accommodating a first reagent containing a substance to label the cells in the sample and a second reagent container accommodating a second reagent different from the first reagent;
a reagent temperature adjustment device configured to adjust temperatures of the first and second reagents in the first and second reagent storages;
a dispenser configured to dispense the first reagent and the second reagent into the sample in the container in the holder;
a display configured to show a selection screen for selecting one of a plurality of modes corresponding to analysis items;
an input device configured to receive a selection of a mode on the selection screen;
a controller storing a temperature setting for each of the plurality of modes and configured to control the sample temperature adjustment device and the reagent temperature adjustment device according to the temperature setting in response to the mode selection, wherein the temperatures of the first and second reagents in the first and second reagent storages are controlled at different temperatures.

2. The pretreatment apparatus according to claim 1 further comprising:
a housing for internally housing the centrifugal separation device;
wherein the sample temperature adjustment device and the reagent temperature adjustment device are provided within the housing.

3. The pretreatment apparatus according to claim 1 , wherein
the selection screen is configured to receive designation of a numerical value indicating a temperature.

4. The pretreatment apparatus according to claim 1 , wherein
the selection screen is configured to receive setting of contents of a plurality of operations included in the pretreatment and a plurality of sample temperatures for the plurality of operations.

5. The pretreatment apparatus according to claim 4 , wherein
the selection screen is configured to receive setting of whether to perform an operation included in the pretreatment while switching from a first sample temperature included in the plurality of sample temperatures to a second sample temperature included in the plurality of sample temperatures.

6. The pretreatment apparatus according to claim 1 , wherein
the selection screen is configured to receive setting of the temperatures of the first reagent and the second reagent.

7. The pretreatment apparatus according to claim 1 , wherein
the controller includes a storage that stores information indicating the sample temperature setting for each of the plurality of modes.

8. The pretreatment apparatus of claim 1 , further comprising:
a first reagent installing part in which the first reagent is installed;
a second reagent installing part in which the second reagent is installed;
wherein the reagent temperature adjustment device adjusts the reagent temperatures in the first reagent installing part and the second reagent installing part;
wherein the input device receives reagent temperature information indicating the temperatures of the first and second reagent installing parts from a user; and
wherein the controller controls the reagent temperature adjustment device according to the temperatures indicated by the reagent temperature information.

9. The pretreatment apparatus according to claim 1 , wherein
the pretreatment further comprises fixing and permeabilizing cells contained in the sample.

10. The pretreatment apparatus according to claim 1 , further comprising:
a housing for internally housing the centrifugal separation device;
wherein the sample temperature adjustment device adjusts an ambient temperature in the housing;
the controller controls a first reaction of the sample and the first reagent in the container within the housing, a centrifugal separation of removing unreacted components in the first reaction, and a second reaction of the sample and the second reagent, and controls a switching of an ambient temperature in the housing by the sample temperature adjustment device such that the first reaction and the second reaction are performed at different temperatures.

11. The pretreatment apparatus according to claim 10 , wherein
the controller executes at least one other operation included in the pretreatment while the ambient temperature in the housing is being switched.

12. The pretreatment apparatus according to claim 10 , wherein
the controller performs controls to start the second reaction after the ambient temperature in the housing is switched.

13. A pretreatment apparatus comprising:
a pretreatment device including a holder that holds a container containing a sample containing cells and configured to perform pretreatment of the sample, the pretreatment including a reaction of the sample and at least a first reagent in the container in the holder;
a sample temperature adjustment device configured to adjust a temperature of the sample in the container in the holder;
a first reagent installing part in which the first reagent is installed;
a first reagent temperature adjustment device configured to adjust a temperature of the first reagent installed in the first reagent installing part;
a second reagent installing part in which a second reagent is installed, wherein the second reagent is different from the first reagent;
a second reagent temperature adjustment device configured to adjust a temperature of the second reagent installed in the second reagent installing part;
an input device configured to receive, from a user, a designated temperature of the sample that is adjusted by the sample temperature adjustment device, a designated temperature of the first reagent that is adjusted by the first reagent temperature adjustment device, and a designated temperature of the second reagent that is adjusted by the second reagent temperature adjustment device;
a controller configured to control the sample temperature adjustment device, the first reagent temperature adjustment device, and the second reagent temperature adjustment device according to the designated temperatures received through the input device, wherein the temperature of the first reagent temperature adjustment device and the second reagent temperature adjustment device are controlled at different temperatures, and
a display that shows a setting screen for designating a temperature,
wherein the input device is further configured to receive a designation of a temperature of the sample that is adjusted by the sample temperature adjustment device from a user for each of a plurality of modes corresponding to analysis items;
wherein the display is further configured to show a selection screen for selecting one of the plurality of modes corresponding to the analysis items; and
wherein the controller is further configured to control the sample temperature adjustment device according to the temperature of the sample designated in the mode selected on the selection screen.

14. A pretreatment apparatus comprising:
a pretreatment device configured to perform pretreatment of a sample, the pretreatment including a reaction of a sample and at least a first reagent;
a sample temperature adjustment device that adjusts a temperature of the

Assignees

• Sysmex Corp

Inventors

• Yamamoto Takeshi
• Sumida Hiroyasu
• Kitawaki Yoshihiko
• Tabata Seiichiro

Classifications

• G01N1/4077 Primary

  by other techniques involving separation of suspended solids · CPC title

• G01N2035/0496

  Other details · CPC title

• G01N2035/00495

  Centrifuges · CPC title

• G01N2035/00346

  Heating or cooling arrangements · CPC title

• G01N35/04 Primary

  Details of the conveyor system {(G01N35/021 - G01N35/028 take precedence)} · CPC title