Polymeric dye ratiometric sensor for analyte detection and methods of using the same

Patent metadata

Field	Value
Publication number	US-11099190-B2
Application number	US-201615780219-A
Country	US
Kind code	B2
Filing date	Dec 6, 2016
Priority date	Dec 16, 2015
Publication date	Aug 24, 2021
Grant date	Aug 24, 2021

Abstract

Fluorogenic sensors for a target analyte are provided. The fluorogenic sensors can include a water soluble light harvesting multichromophore and a fluorogenic dye covalently linked to the multichromophore and in energy-receiving proximity therewith. Aspects of the fluorogenic sensors include selective recognition of the target analyte by chemical reaction or by specific binding. In some cases, the linked fluorogenic dye includes a lactone or spiro-lactone group that is configured to chemically react with a target analyte via lactone ring-opening to produce a fluorescent dye configured for excitation by the multichromophore. Also provided are labelled specific binding members that include the subject dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject fluorogenic sensors find use are provided. Systems, devices and kits for practicing the subject methods are provided.

First claim

What is claimed is:

1. A fluorogenic sensor for hydroxide, the sensor comprising a water soluble light harvesting multichromophore and a linked fluorogenic dye in energy-receiving proximity therewith, wherein the sensor is described by formula (X):

wherein:
Z 1 is the linked fluorogenic dye in energy receiving proximity to the multichromophore;
F 1 is a fluorene co-monomer;
each M 1 and each M 2 is independently a co-monomer;
L 1 is a fluorene co-monomer substituted with the linked fluorogenic dye (Z 1 );
e is 1;
a, b, c, d and f are each independently 0 or 1, wherein a+b+c+d+f≥1;
n is 0 or an integer from 1 to 10,000;
m is an integer from 1 to 10,000;
p is an integer from 1 to 100,000; and
G 1 and G 2 are each independently selected from a terminal group, a π conjugated segment, a linker and a linked specific binding member;

wherein the fluorogenic dye has the structure

wherein L is a linker covalently attached to the multichromophore.

2. The fluorogenic sensor according to claim 1 , wherein the linked fluorogenic dye is non-fluorescent in the absence of target analyte when the sensor is irradiated with incident excitation light at the absorption maxima wavelength of the multichromophore.

3. The fluorogenic sensor according to claim 1 , wherein the linked fluorogenic dye quenches 10% or more of the fluorescence of the multichromophore.

4. The fluorogenic sensor according to claim 1 , wherein the fluorescence ratio of the fluorescent versus non-fluorescent forms of the sensor is 10 or more.

5. The fluorogenic sensor according to claim 1 , described by formula (II):

6. The fluorogenic sensor according to claim 1 , described by formula (III):

7. A method of evaluating a sample for the presence of hydroxide, the method comprising:
(a) contacting the sample with the fluorogenic sensor according to claim 1 to produce a sensor-contacted sample;
(b) evaluating the sensor-contacted sample for fluorescence emission from the fluorogenic sensor to evaluate whether hydroxide is present in the sample.

8. The method according to claim 7 , wherein the fluorogenic sensor is support bound.

9. The method according to claim 8 , wherein the support comprises a magnetic particle.

10. The method according to claim 7 , wherein said contacting is carried out in vitro.

11. The method according to claim 7 , wherein said contacting is carried out in vivo.

12. The method according claim 7 , wherein the evaluating comprises measuring a ratiometric change in fluorescence of the sensor relative to a control.

Assignees

• Becton Dickinson Co

Inventors

• Martin Jody
• Radford Robert J
• Ghadiali James

Classifications

• C09B11/22

  containing OH groups bound to an aryl nucleus {and their ethers and esters} · CPC title

• G01N33/542

  with steric inhibition or signal modification, e.g. fluorescent quenching · CPC title

• G01N33/53

  Immunoassay; Biospecific binding assay; Materials therefor · CPC title

• C07K2317/55

  Fab or Fab' · CPC title

• G01N33/582 Primary

  with fluorescent label · CPC title