Protein sensor and manufacturing method thereof

Patent metadata

Field	Value
Publication number	US-11099184-B2
Application number	US-202016879790-A
Country	US
Kind code	B2
Filing date	May 21, 2020
Priority date	Nov 19, 2019
Publication date	Aug 24, 2021
Grant date	Aug 24, 2021

Abstract

A manufacturing method of a protein sensor includes the following steps. A hydrophobic material is provided and the hydrophobic material has a surface. An atmospheric pressure plasma process is performed to form a hydrophilic functional group on the surface of the hydrophobic material. A first antibody is immobilized on the surface of the hydrophobic material by the hydrophilic functional group. A mixed solution is prepared. The mixed solution includes a second antibody and an analyte, and the second antibody binds to the analyte. The mixed solution is reacted with the first antibody immobilized on the surface of the hydrophobic material to bind the first antibody to the analyte. Furthermore, a protein sensor manufactured by the above-described manufacturing method of the protein sensor is also provided.

First claim

What is claimed is:

1. A manufacturing method of a protein sensor, comprising:
providing a hydrophobic material, wherein the hydrophobic material comprises a surface;
performing an atmospheric pressure plasma process to form a hydrophilic functional group on the surface of the hydrophobic material;
immobilizing a first antibody on the surface of the hydrophobic material by the hydrophilic functional group;
preparing a mixed solution, wherein the mixed solution comprises a second antibody and an analyte, and the second antibody binds to the analyte; and
reacting the mixed solution with the first antibody immobilized on the surface of the hydrophobic material to bind the first antibody to the analyte,
wherein the atmospheric pressure plasma process is a wet atmospheric pressure plasma modification method or a dry atmospheric pressure plasma modification method,
wherein the wet atmospheric pressure plasma modification method comprises the following steps:
dispersing the hydrophobic material in deionized water; and
applying an atmospheric pressure plasma with a current of 10 mA to the surface of the hydrophobic material for 3 min,

wherein the dry atmospheric pressure plasma modification method comprises the following step:
applying an atmospheric pressure plasma with a power of 500 W and a frequency of 32 Hz to the surface of the hydrophobic material.

2. The manufacturing method of the protein sensor according to claim 1 , wherein the hydrophobic material comprises graphite, graphene, or a gold sheet.

3. The manufacturing method of the protein sensor according to claim 1 , wherein the hydrophilic functional group comprises a carboxyl group, a hydroxyl group, or a combination thereof.

4. The manufacturing method of the protein sensor according to claim 1 , wherein the step of immobilizing the first antibody to the surface of the hydrophobic material by the hydrophilic functional group comprises: bonding an amino acid of the first antibody to the hydrophilic functional group on the surface of the hydrophobic material.

5. The manufacturing method of the protein sensor according to claim 1 , wherein a source of the analyte comprises a cell extract or serum.

6. The manufacturing method of the protein sensor according to claim 1 , wherein the analyte is a tumor marker CA125.

7. A protein sensor, manufactured by the manufacturing method of the protein sensor according to claim 1 .

8. The protein sensor according to claim 7 , wherein the protein sensor comprises a lowest detection limit of 2.7×10 −2 ng/mL to 3.2 ng/mL.

Assignees

• Univ Nat Taiwan Science & Technology

Inventors

• Wang Fu-Ming
• Kuo Yu-Lin
• Chen wei-ling
• Wang xing-chun
• Yuan Chiou-Chung

Classifications

• G01N33/57575

  involving oncogenic proteins · CPC title

• G01N33/68 Primary

  involving proteins, peptides or amino acids {(involving lipoproteins G01N33/92)} · CPC title

• G01N33/5438 Primary

  Electrodes · CPC title

• G01N33/54353

  with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393) · CPC title

• G01N33/5748

  Physics · mapped topic