Detection apparatus and detection method

Patent metadata

Field	Value
Publication number	US-11099182-B2
Application number	US-201715637957-A
Country	US
Kind code	B2
Filing date	Jun 29, 2017
Priority date	Jun 30, 2016
Publication date	Aug 24, 2021
Grant date	Aug 24, 2021

Abstract

Disclosed is a detection apparatus that transfers magnetic particles through a plurality of chambers in a cartridge which includes the plurality of chambers and a channel connecting between the plurality of chambers, and that causes the magnetic particles to carry a complex of a test substance and a labelling substance, to detect the test substance on the basis of the labelling substance in the complex. The detection apparatus includes: a rotation mechanism configured to rotate the cartridge about a rotation shaft; a magnet configured to collect the magnetic particles in the chambers; a movement mechanism configured to move the magnet in a direction different from a circumferential direction of a circle in which the rotation shaft is centered; a detector configured to detect the test substance; and a controller programmed to control the rotation mechanism and the movement mechanism so as to transfer the magnetic particles from one of the chambers to another one of the chambers.

First claim

What is claimed is:

1. A detection apparatus that transfers magnetic particles through a plurality of chambers in a cartridge which comprises the plurality of chambers and a channel connecting between the plurality of chambers, and that causes the magnetic particles to carry a complex of a test substance and a labelling substance, to detect the test substance on the basis of the labelling substance in the complex, the detection apparatus comprising:
a rotation mechanism comprising a driving source and configured to rotate the cartridge about a rotation shaft;
a magnet configured to collect the magnetic particles in the chambers;
a movement mechanism comprising a driving source and configured to move the magnet in a direction different from a circumferential direction of a circle in which the rotation shaft is centered;
a detector configured to detect the test substance; and
a controller programmed to control:
the rotation mechanism to rotate the cartridge while the magnetic particles are attracted by the magnet; and
the movement mechanism to move the magnet attracting the magnetic particles while the cartridge is not rotated so as to transfer the magnetic particles from one of the chambers to another one of the chambers.

2. The detection apparatus of claim 1 , wherein
the movement mechanism moves the magnet in a radial direction of a circle in which the rotation shaft is centered.

3. The detection apparatus of claim 1 , wherein
the plurality of chambers comprise a first chamber and a second chamber, and
the controller is programmed to control the movement mechanism so as to move the magnet from the first chamber to the channel.

4. The detection apparatus of claim 3 , wherein
the controller is programmed to control the rotation mechanism so as to move the magnet relative to the channel.

5. The detection apparatus of claim 3 , wherein
the controller is programmed to control to move the magnet from the channel to the second chamber.

6. The detection apparatus of claim 3 , wherein
the first chamber and the second chamber are disposed so as to be aligned in the circumferential direction.

7. The detection apparatus of claim 3 , wherein
the channel comprises a first region that extends in a radial direction of a circle in which the rotation shaft is centered, and that connects to the first chamber, and
the controller is programmed to control the movement mechanism so as to move the magnet along the first region.

8. The detection apparatus of claim 3 , wherein
the channel comprises a second region that extends in a radial direction of a circle in which the rotation shaft is centered, and that connects to the second chamber, and
the controller is programmed to control the movement mechanism so as to move the magnet along the second region.

9. The detection apparatus of claim 3 , wherein
the channel comprises a third region that extends in the circumferential direction of a circle in which the rotation shaft is centered, and
the controller is programmed to control the rotation mechanism so as to relatively move the magnet along the third region.

10. The detection apparatus of claim 1 , wherein
the chamber comprises a liquid phase region in which liquid is stored,
the channel comprises a gas phase region in which gas is stored, and
the controller is programmed to control the rotation mechanism and the movement mechanism so as to transfer the magnetic particles through the gas phase region of the channel from one of the chambers to the liquid phase region of another one of the chambers.

11. The detection apparatus of claim 1 , wherein
the controller is programmed to control the movement mechanism so as to move the magnet close to the chamber.

12. The detection apparatus of claim 11 , wherein
the plurality of chambers comprise a first chamber and a second chamber, and
the controller is programmed to control the rotation mechanism and the movement mechanism such that the magnet is moved close to the first chamber, the magnet is thereafter moved along the channel and positioned in the second chamber, and the magnet is thereafter moved so as to be distant from the second chamber.

13. The detection apparatus of claim 1 , wherein
the magnet is tapered such that the closer a cartridge side portion of the magnet is to the cartridge, the less a cross-sectional area of the magnet is.

14. The detection apparatus of claim 13 , wherein
the magnet has a conic shape on the cartridge side.

15. The detection apparatus of claim 13 , wherein
a tip portion of the magnet on the cartridge side has a columnar shape having a constant cross-sectional area.

16. The detection apparatus of claim 13 , wherein
a width of an edge of the magnet is less than a minimal width of the channel.

17. The detection apparatus of claim 13 , wherein
the magnet is formed by a magnetic body being joined to a permanent magnet.

18. The detection apparatus of claim 1 , wherein
the plurality of chambers comprise a first chamber, a second chamber, a third chamber, and a fourth chamber,
in the first chamber, the test substance and the magnetic particles bind to each other to generate a complex,
in the second chamber, a labelling substance binds to the complex generated in the first chamber,
in the third chamber, the complex having the labelling substance bound thereto is mixed with washing liquid for separating an unreacted substance from the complex having the labelling substance bound thereto,
in the fourth chamber, the complex having the labelling substance bound thereto, is mixed with a luminescent reagent that generates light by reaction with the labelling substance bound to the complex, and
the controller is programmed to control the rotation mechanism and the movement mechanism so as to transfer the magnetic particles to the first chamber, the second chamber, the third chamber, and the fourth chamber in order, respectively.

19. The detection apparatus of claim 1 , comprising
a support member on which the cartridge is placed, wherein
the rotation mechanism rotates the cartridge by rotating the support member about the rotation shaft.

20. The detection apparatus of claim 19 , wherein
the cartridge has a liquid storage portion having a seal,
the detection apparatus comprises a pressing portion configured to press the seal, and
the support member is positioned so as to oppose the pressing portion across the cartridge.

21. The detection apparatus of claim 20 , wherein
the support member is positioned from a position on the rotation shaft side to a position opposing the pressing portion.

22. The detection apparatus of claim 1 , wherein
each chamber of the plurality of chambers is connected to the channel on the rotation shaft side, and
each chamber of the plurality of chambers comprises protrusions that protrude toward the rotation shaft, and that are disposed on both sides which sandwich a connecting position at which each chamber of the plurality of chambers connects to the channel.

23. The detection apparatus of claim 22 , wherein
each chamber of the plurality of chambers comprises planer wall surfaces that are connected to the protrusions, respectively, and that are disposed on both sides which sandwich the connecting position.

24. The detection apparatus of claim 1 , wherein
each chamber of the plurality of chambers comprises a projection that projects toward the rotation shaft, and
the channel is connec

Assignees

• Sysmex Corp

Inventors

• Horii Kazuyoshi
• Nose Tomoyuki
• Fujiwara Takao
• Tomoda Sayuri
• Miida Yusuke
• Maekawa Yasunori
• Sasaki Noriaki

Classifications

• G01N2035/0439

  Rotary sample carriers, i.e. carousels · CPC title

• G01N35/04

  Details of the conveyor system {(G01N35/021 - G01N35/028 take precedence)} · CPC title

• G01N33/532 Primary

  Production of labelled immunochemicals · CPC title

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• B01L2200/0668

  Trapping microscopic beads · CPC title