Performing optical measurements on a sample

Patent metadata

Field	Value
Publication number	US-11099175-B2
Application number	US-201716099270-A
Country	US
Kind code	B2
Filing date	May 11, 2017
Priority date	May 11, 2016
Publication date	Aug 24, 2021
Grant date	Aug 24, 2021

Abstract

Apparatus and methods are described for use with a blood sample ( 48, 50 ), including measuring hemoglobin concentration within at least a portion ( 48 ) of the blood sample, by performing a first measurement on the blood sample. Mean corpuscular hemoglobin in the blood sample ( 48, 50 ) is measured, by performing a second measurement on the blood sample ( 48, 50 ). A parameter of the blood sample is determined, based on a relationship between the concentration of hemoglobin and the mean corpuscular hemoglobin. Other applications are also described.

First claim

The invention claimed is:

1. A method for use with a blood sample, the method comprising:
measuring hemoglobin concentration within at least a portion of the blood sample, by performing a first measurement on a first portion of the blood sample;
measuring mean corpuscular hemoglobin in the blood sample, by performing a second measurement on a second portion of the blood sample, the second portion being diluted with respect to the first portion;
determining a red blood cell count per unit volume within the blood sample, by dividing the concentration of hemoglobin measured within the first portion by the mean corpuscular hemoglobin measured within the second portion; and
determining counts of one or more components per unit volume within the blood sample, based on the red blood cell count per unit volume within the blood sample, by:
analyzing a microscopic image of at least one of the first and second portions of the blood sample, such as to determine a count of the one or more components and a count of red blood cells within the at least one of the first and second portions of the blood sample, determining a ratio between the count of the one or more components and the count of red blood cells within the at least one of the first and second portions of the blood sample, and
determining the count of the one or more components per unit volume within the blood sample by multiplying the red blood cell count per unit volume by the ratio.

2. The method according to claim 1 , wherein performing the first measurement on the first portion of the blood sample comprises performing an optical density measurement on the blood sample.

3. The method according to claim 1 , wherein the one or more components within the blood sample are selected from the group consisting of: red blood cells, red blood cells of a given type, white blood cells, white blood cells of a given type, circulating tumor cells, platelets, platelets of a given type, bacteria, pathogens, pathogens of a given type, reticulocytes, and Howell-Jolly bodies.

4. The method according to claim 1 , wherein analyzing the microscopic image of at least one of the first and second portions of the blood sample comprises analyzing a microscopic image of the first portion of the blood sample.

5. The method according to claim 1 , wherein analyzing the microscopic image of at least one of the first and second portions of the blood sample comprises analyzing a microscopic image of the second portion of the blood sample.

6. The method according to claim 1 , wherein performing a second measurement on the second portion of the blood sample comprises performing optical density measurements on a cellular level on the second portion of the blood sample.

7. The method according to claim 6 , wherein performing optical density measurements on a cellular level on the second portion of the blood sample comprises performing microscopic bright-field imaging on the second portion of the blood sample.

8. Apparatus for use with a blood sample, the apparatus comprising: at least one computer processor configured to:
measure hemoglobin concentration within at least a portion of the blood sample, by performing a first measurement on a first portion of the blood sample,
measure mean corpuscular hemoglobin in the blood sample, by performing a second measurement on a second portion of the blood sample, the second portion being diluted with respect to the first portion,
determine a red blood cell count per unit volume within the blood sample, by dividing the concentration of hemoglobin measured within the first portion by the mean corpuscular hemoglobin measured within the second portion, and
determining counts of one or more components per unit volume within the blood sample, based on the red blood cell count per unit volume within the blood sample, by:
analyzing a microscopic image of at least one of the first and second portions of the blood sample, such as to determine a count of the one or more components and a count of red blood cells within the at least one of the first and second portions of the blood sample, determining a ratio between the count of the one or more components and the count of red blood cells within the at least one of the first and second portions of the blood sample, and
determining the count of the one or more components per unit volume within the blood sample by multiplying the red blood cell count per unit volume by the ratio.

9. The apparatus according to claim 8 , wherein the at least one computer processor is configured to perform the first measurement on the first portion of the blood sample by performing an optical density measurement on the blood sample.

10. The apparatus according to claim 8 , wherein the one or more components within the blood sample are selected from the group consisting of: red blood cells of a given type, white blood cells, white blood cells of a given type, circulating tumor cells, platelets, platelets of a given type, bacteria, pathogens, pathogens of a given type, reticulocytes, and Howell-Jolly bodies.

11. The apparatus according to claim 8 , wherein the at least one computer processor is configured to analyze the microscopic image of at least one of the first and second portions of the blood sample by analyzing a microscopic image of the first portion of the blood sample.

12. The apparatus according to claim 8 , wherein the at least one computer processor is configured to analyze the microscopic image of at least one of the first and second portions of the blood sample by analyzing a microscopic image of the second portion of the blood sample.

13. The apparatus according to claim 8 , wherein the at least one computer processor is configured to perform the second measurement on the second portion of the blood sample by performing optical density measurements on a cellular level on the second portion of the blood sample.

14. The apparatus according to claim 13 , wherein the at least one computer processor is configured to perform the optical density measurements on a cellular level on the second portion of the blood sample by performing microscopic bright-field imaging on the second portion of the blood sample.

15. A computer software product, for use with a blood sample, the computer software product comprising a non-transitory computer-readable medium in which program instructions are stored, which instructions, when read by a computer cause the computer to perform the steps of:
measuring hemoglobin concentration within a first portion of the blood sample, by performing a first measurement on the first portion of the blood sample;
measuring mean corpuscular hemoglobin in the blood sample, by performing a second measurement on a second portion of the blood sample, the second portion being diluted with respect to the first portion;
determining a red blood cell count per unit volume within the blood sample, by dividing the concentration of hemoglobin measured within the first portion by the mean corpuscular hemoglobin measured within the second portion: and
determining counts of one or more components per unit volume within the blood sample, based on the red blood cell count per unit volume within the blood sample, by:
analyzing a microscopic image of at least one of the first and second portions of the blood sample, such as to determine a count of the one or more components and a count of red blood cells within the at least one of the first and second portions of the blood sample, determining a ratio between the count of the one or more components and the count of red blood cells within the at least one of the first and second portions of the

Assignees

• S D Sight Diagnostics Ltd

Inventors

• Zait Amir
• Houri Yafin Arnon
• Gluck Dan
• Pecker Sharon
• Eshel Yochay Shlomo
• Levy Schreier Sarah
• Pollak Joseph Joel

Classifications

• G01N2021/8592

  Grain or other flowing solid samples · CPC title

• G01N2015/1006

  for cytology · CPC title

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title

• G01N21/59

  Transmissivity (G01N21/25 takes precedence) · CPC title

• G01N15/10 Primary

  Investigating individual particles · CPC title