Antibodies with engineered CH2 domains, compositions thereof and methods of using the same

Patent metadata

Field	Value
Publication number	US-11098107-B2
Application number	US-201716309821-A
Country	US
Kind code	B2
Filing date	Jun 14, 2017
Priority date	Jun 15, 2016
Publication date	Aug 24, 2021
Grant date	Aug 24, 2021

Abstract

The present disclosure relates to antibodies and antibody conjugates having one or more site-specific mutations in the CH2 domain of the heavy chain. The antibody variants disclosed herein can have improved characteristics (e.g., thermal stability, antibody yields, antibody titers, cell-killing) relative to a parent or wild type antibody, including aglycosylated parent or wild type antibodies. Pharmaceutical compositions, diagnostic compositions and kits comprising the same, as well as methods of using these compositions and kits for therapeutic and diagnostic purposes, are also described.

First claim

What is claimed:

1. An antibody of the IgG class with amino acid substitutions in the CH2 domain of the heavy chain comprising:
V262E, V264S, and V303R;
V262E, V264S, and V305R;
V262E, V303R, and V305R; or
V262E, V264S, V303R and V305R.

2. The antibody of claim 1 , further comprising an amino acid substitution at position F241 and/or F243 of the CH2 domain.

3. The antibody of claim 1 , wherein the antibody is aglycosylated.

4. The antibody of claim 1 , further comprising one or more non-natural amino acid residues, wherein the one or more non-natural amino acid residues comprises a moiety selected from the group consisting of amino, carboxy, acetyl, hydrazine hydrazido, semicarbazido, sulfanyl, azido, alkynyl, and tetrazine.

5. The antibody of claim 1 , wherein the antibody is a bi-specific antibody.

6. The antibody of claim 1 , that is of the IgG1 subclass.

7. The antibody of claim 1 , that is of the IgG2 subclass.

8. The antibody of claim 1 that is of the IgG4 subclass.

9. The antibody of claim 1 that is in a form selected from the group consisting of Fc, and full-length antibody.

10. The antibody of claim 4 , wherein at least one non-natural amino acid is para-azido phenylalanine or para-azido methyl phenylalanine.

11. The antibody of claim 4 comprising two or more non-natural amino acid residues.

12. The antibody of claim 11 , wherein a second non-natural amino acid residue is para-azido phenylalanine or para-azido methyl phenylalanine.

13. The antibody of claim 11 , wherein at least one non-natural amino acid residue comprises a tetrazine moiety and at least second non-natural amino acid residue comprises an azide moiety.

14. An antibody conjugate comprising the antibody of claim 4 linked to one or more therapeutic moieties or labeling moieties by one or more non-natural amino acid residues.

15. The antibody conjugate of claim 14 , wherein at least one of said therapeutic moieties or labeling moieties is linked to said antibody via a residue of a non-natural amino acid comprising an azide moiety.

16. The antibody conjugate of claim 14 , wherein at least one of said therapeutic moieties or labeling moieties is linked to said antibody via a residue of the non-natural amino acid comprising a tetrazine moiety.

17. The antibody conjugate of claim 14 , wherein at least one of said therapeutic moieties or labeling moieties is linked to said antibody via a residue of the non-natural amino acid comprising an azide moiety and at least one of said therapeutic moieties or labeling moieties is linked to said antibody via a residue of the non-natural amino acid comprising a tetrazine moiety.

18. The antibody conjugate of claim 14 , wherein said antibody is linked to said one or more therapeutic moieties or labeling moieties via one or more linkers.

19. A composition comprising the antibody of claim 1 , wherein said antibody is substantially pure.

20. A composition comprising the antibody of claim 1 wherein said antibody is at least 95% by mass of the total antibody mass of said composition.

21. A kit comprising an antibody of claim 1 , and instructions for use of the antibody.

22. A kit comprising an antibody conjugate of claim 14 , and instructions for use of the antibody.

23. A polynucleotide encoding an antibody of claim 1 .

24. A vector comprising the polynucleotide of claim 23 .

25. A host cell comprising the vector of claim 24 .

Assignees

• Sutro Biopharma Inc

Inventors

• Calarese Daniel
• Yin Gang

Classifications

• C07K16/00 Primary

  Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies · CPC title

• C07K16/18 Primary

  against material from animals or humans · CPC title

• C07K2317/90

  characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin · CPC title

• A61P35/00

  Antineoplastic agents · CPC title

• C12N2015/8518

  expressing industrially exogenous proteins, e.g. for pharmaceutical use, human insulin, blood factors, immunoglobulins, pseudoparticles · CPC title