Device for manipulation of packets in micro-containers, in particular in microchannels

Patent metadata

Field	Value
Publication number	US-11097275-B2
Application number	US-202016784736-A
Country	US
Kind code	B2
Filing date	Feb 7, 2020
Priority date	Sep 9, 2004
Publication date	Aug 24, 2021
Grant date	Aug 24, 2021

Abstract

A microfluidic device for performing physical, chemical or biological treatment to at least one packet without contamination.

First claim

The invention claimed is:

1. A device for performing a PCR, comprising:
a microchannel comprising a coil comprising a capillary tube defining an internal space of the microchannel, wherein the capillary tube comprises a bulk fluorinated material that is non-internally coated, wherein the capillary tube is at least partly filled with:
a carrier fluid comprising a fluorosolvent containing a surfactant, and
aqueous droplets surrounded by the carrier fluid,

the coil comprising a denaturing region and an annealing region, both regions being at different temperatures, wherein the difference between an interfacial tension between a droplet and the capillary tube and an interfacial tension between the droplet and the carrier fluid is at least 26 mN/m.

2. The device according to claim 1 , wherein the bulk fluorinated material is a fluoropolymer.

3. The device according to claim 1 , comprising a cylinder comprising three regions corresponding to the denaturing, annealing and elongation regions.

4. The device according to claim 3 , the capillary tube being wound around the cylinder.

5. The device according to claim 3 , the denaturing, annealing and elongation regions being isolated one from another by sheets, which are affixed between the pieces of the cylinder.

6. The device according to claim 3 , the size of the elongation region being twice the size of the denaturing and annealing regions.

7. The device according to claim 1 , the device having a cylinder shape and comprising three ventilation holes per quarter cylinder, said holes being drilled through the entire device to provide vents for air cooling.

8. The device according to claim 7 , further comprising a turbine configured to blow ambient air through the ventilation holes providing temperature control and uniformity.

9. The device according to claim 1 , the device having a cylinder shape, two holes for receiving thermocouples and one central hole for receiving a heater being drilled partially through the cylinder in each temperature region, the central hole being larger than the holes for receiving the thermocouples.

10. The device according to claim 1 , each region comprising a resistance heater and two thermocouples.

11. The device according to claim 10 , the resistance heaters being located in the center of their respective region, and the thermocouples being located near the interface between regions.

12. The device according to claim 1 , the capillary tube comprising a DNA fragment.

13. The device according to claim 1 , wherein the coil comprises an elongation region being at a temperature different from the temperature of the denaturing and the annealing regions.

Assignees

• Inst Curie
• Centre Nat Rech Scient
• Centre Nationale De Recherche Scient

Inventors

• Viovy Jean-Louis
• Chabert Max
• Dorfman Kevin

Classifications

• B01F33/3031

  using electro-hydrodynamic [EHD] or electro-kinetic [EKI] phenomena to mix or move the fluids · CPC title

• B01F33/3021

  the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets · CPC title

• G01N2015/003

  in liquids, e.g. emulsion · CPC title

• B01J2219/00824

  Ceramic · CPC title

• B01L3/502723

  characterised by venting arrangements · CPC title