Multiple tissue layer electroporation applicator and device

Patent metadata

Field	Value
Publication number	US-11097099-B2
Application number	US-201113581700-A
Country	US
Kind code	B2
Filing date	Mar 1, 2011
Priority date	Mar 1, 2010
Publication date	Aug 24, 2021
Grant date	Aug 24, 2021

Abstract

The present invention relates to dual depth electroporation devices capable of electorporating both muscle tissue and skin tissue in a single application in order to generate a broad immune response in a subject, and uses of the same.

First claim

What is claimed is:

1. A dual depth electroporation device capable of electroporating both muscle tissue and skin tissue in a single application, comprising:
an electrical pulse generator;
an array in electrical communication with said electrical pulse generator, said array having a first set of electrodes and a second set of electrodes;
wherein the first set of electrodes is for intramuscular delivery of a first electric pulse, the first set of electrodes having a length of about 10 mm to about 35 mm, and wherein the second set of electrodes is for intradermal delivery of a second electric pulse, the second set of electrodes having a length of about 0.01 mm to about 4 mm,
and wherein the array is configured such that the first set of electrodes is positioned for intramuscular delivery at the same time the second set of electrodes is positioned for intradermal delivery.

2. The device of claim 1 , wherein the first electric pulse is delivered to muscle tissue and the second electric pulse is delivered to skin tissue.

3. The device of claim 2 , wherein the electrical pulse generator delivers through the first set of electrodes the first electric pulse of from 1 volt to about 200 volts and the electrical pulse generator delivers through the second set of electrodes the second electric pulse of from 1 volt to about 200 volts.

4. The device of claim 3 , wherein the electrical pulse generator delivers through the second set of electrodes the second electric pulse of from 1 volt to about 50 volts.

5. The device of claim 2 , wherein the electrical pulse generator delivers through the first set of electrodes the first electric pulse of about 100 volts and the electrical pulse generator delivers through the second set of electrodes the second electric pulse of about 15 volts.

6. The device of claim 2 , wherein each first electric pulse and the second electric pulse, individually, is repeated up to 6 times.

7. The device of claim 2 , wherein the first electric pulse is repeated two times, and the second electric pulse is repeated three times.

8. The device of claim 2 , wherein sequentially the first electric pulse occurs first and the second electric pulse occurs second.

9. The device of claim 2 , wherein the first electric pulse delivers a current of from about 50 mA to about 500 mA, and the second electric pulse delivers a current of from about 50 mA to about 500 mA or from about 1 mA to about 50 mA.

10. The device of claim 2 , wherein the first electric pulse delivers a current of about 400 mA, and the second electric pulse delivers a current of about 100 mA or about 10 mA.

11. The device of claim 2 , wherein each of the first electric pulse and second electric pulse, individually, has a duration from 5 msec to about 250 msec.

12. The device of claim 2 , wherein the second set of electrodes have a sharp distal tip that allows penetration of the skin tisue through the stratum corneum into the underlying epidermal tissue above the basal layer.

13. A method of generating a broad immune response in a subject by administering a biomolecule to both muscle tissue and skin tissue by electroporation assisted delivery using a dual depth electroporation device according to claim 1 , comprising:
injecting a biomolecule to both muscle and skin tissue;
inserting the array, comprising inserting the first set of electrodes into muscle tissue and inserting the second set of electrodes into skin tissue;
delivering through the first set of electrodes the first electric pulse to the muscle tissue and through the second set of electrodes the second electric pulse to the skin tissue to permit transfection of the biomolecule; and
allowing subject's immune system to generate broad immune response including humoral and cellular response to the injected biomolecule.

14. The method of claim 13 , wherein the injecting step occurs prior to the inserting step.

15. The method of claim 13 , wherein the delivering step comprises delivering through the first set of electrodes the first electric pulse of from 1 volt to about 200 volts and delivering through the second set of electrodes the second electric pulse of from 1 volt to about 200 volts.

16. The method of claim 15 , wherein the delivering step comprises delivering through the second set of electrodes the second electric pulse of from 1 volt to about 50 volts.

17. The method of claim 13 , wherein the delivering step comprises repeating each of the delivering of the first electric pulse and the delivering of the second electric pulse, individually, up to 6 times.

18. The method of claim 13 , wherein the delivering step comprises, sequentially, the delivering of the first electric pulse first and the delivering of the second electric pulse second.

19. The method of claim 13 , wherein the delivering step comprises the first electric pulse delivering a current of from about 50 mA to about 500 mA, and the second electric pulse delivering a current of from about 50 mA to about 500 mA or from about 1 mA to about 50 mA.

20. The method of claim 13 , wherein the delivering step comprises the first electric pulse delivering a current of about 400 mA, and the second electric pulse delivering a current of about 100 mA or about 10 mA.

21. The method of claim 13 , wherein the delivering step comprises delivering the first electric pulse for a duration from 5 msec to about 250 msec and delivering the second electric pulse for a duration from 5 msec to about 250 msec.

Assignees

• Broderick Kate
• Lin Feng
• Mccoy Jay
• Kemmerrer Stephen V
• Kjeken Rune
• Inovio Pharmaceuticals Inc

Inventors

• Broderick Kate
• Lin Feng
• Mccoy Jay
• Kemmerrer Stephen V
• Kjeken Rune

Classifications

• A61N1/327 Primary

  for enhancing the absorption properties of tissue, e.g. by electroporation · CPC title