Method and device for preparing measurement sample for MALDI mass spectrometry, measurement sample for MALDI mass spectrometry, MALDI mass spectrometry method, and non-transitory recording medium for preparing measurement sample for MALDI mass spectrometry

Patent metadata

Field	Value
Publication number	US-11094517-B2
Application number	US-202016801424-A
Country	US
Kind code	B2
Filing date	Feb 26, 2020
Priority date	Mar 20, 2019
Publication date	Aug 17, 2021
Grant date	Aug 17, 2021

Abstract

A method for preparing a measurement sample for MALDI mass spectrometry, the method including applying a laser beam to a base containing a matrix disposed on a surface of the base, in a manner that the laser beam is applied to a surface of the base opposite to the surface on which the matrix is disposed, to make the matrix fly from the base to be disposed at a predetermined position of an analyte of MALDI mass spectrometry.

First claim

The invention claimed is:

1. A method for preparing a measurement sample for MALDI mass spectrometry, the method comprising:
applying a laser beam to a first base containing a matrix disposed on a first surface of the first base, in a manner that the laser beam is applied to a second surface of the first base opposite to the first surface on which the matrix is disposed, to make the matrix fly from the first base to be transferred to a predetermined position of an analyte of MALDI mass spectrometry, the analyte being separately disposed on a surface of a second base facing the first surface of the first base.

2. The method according to claim 1 , wherein the matrix made to fly from the base includes two or more kinds of matrices.

3. The method according to claim 2 , wherein the two or more kinds of matrices made to fly from the base are transferred to mutually different predetermined positions of the analyte of MALDI mass spectrometry.

4. The method according to claim 1 , wherein the matrix is made to fly twice or more times from the base to be transferred to the predetermined position of the analyte of MALDI mass spectrometry.

5. The method according to claim 1 , wherein the laser beam is an optical vortex laser beam.

6. The method according to claim 1 , wherein an irradiation diameter of the laser beam is 5 μm or greater but 100 μm or less.

7. The method according to claim 1 , wherein the matrix disposed on the surface of the base is in a state of a layer or dots or in a state of both a layer and dots.

8. A device for preparing a measurement sample for MALDI mass spectrometry, comprising:
an irradiation unit configured to apply the laser beam to the second surface of the first base based on the method according to claim 1 .

9. The method of claim 1 , further comprising:
receiving irradiation data including position information of the analyte, and moving an irradiation position of the laser beam based on the received position information.

10. A measurement sample for MALDI mass spectrometry, the measurement sample comprising:
an analyte of MALDI mass spectrometry; and
two or more kinds of matrices disposed at predetermined positions of the analyte.

11. A MALDI mass spectrometry method comprising:
performing MALDI mass spectrometry with the measurement sample for MALDI mass spectrometry according to claim 10 .

12. A non-transitory recording medium for preparing a measurement sample for MALDI mass spectrometry, the non-transitory recording medium storing a plurality of instructions which, when executed by processing circuitry, cause the processing circuitry to perform a method, comprising:
based on position information of an analyte of MALDI mass spectrometry, applying a laser beam to a first base containing a matrix disposed on a first surface of the first base, in a manner that the laser beam is applied to a second surface of the first base opposite to the first surface on which the matrix is disposed, to make the matrix fly from the first base to be transferred to a predetermined position of the analyte of MALDI mass spectrometry, the analyte being separately disposed on a surface of a second base facing the first surface of the first base.

Assignees

• Ricoh Co Ltd

Inventors

• Uematsu Katsuyuki
• Suzuki Kazumi

Classifications

• G01N2001/2873

  Cutting or cleaving · CPC title

• H01J49/0418 Primary

  for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates · CPC title

• G01N27/628

  and a beam of energy, e.g. laser enhanced ionisation · CPC title

• H01J49/0031

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title

• G01N1/42

  Low-temperature sample treatment, e.g. cryofixation · CPC title