Carbon fixation systems and methods

Patent metadata

Field	Value
Publication number	US-11091781-B2
Application number	US-202016793577-A
Country	US
Kind code	B2
Filing date	Feb 18, 2020
Priority date	Sep 14, 2015
Publication date	Aug 17, 2021
Grant date	Aug 17, 2021

Abstract

Systems and methods for fixing carbon using bacteria are described. In one embodiment, a system includes a reactor chamber with a solution contained therein. The solution may include hydrogen (H2), carbon dioxide (CO2), bioavailable nitrogen, and a chemolithoautotrophic bacteria. The system may also include a pair of electrodes that split water contained within the solution to form the hydrogen. Additionally, the system may be operated so that a concentration of the bioavailable nitrogen in the solution is below a threshold nitrogen concentration to cause the chemolithoautotrophic bacteria to produce a product.

First claim

What is claimed is:

1. A method comprising:
splitting water using a cathode including a cobalt-phosphorus alloy and an anode including cobalt phosphate in a solution containing a chemolithoautotrophic bacteria to form hydrogen (H 2 ) and oxygen (O 2 ) in the solution;
providing carbon dioxide (CO 2 ) in the solution;
providing bioavailable nitrogen in the solution;
maintaining the bioavailable nitrogen in the solution to below a threshold nitrogen concentration to control production of a product by the chemolitoautrophic bacteria.

2. The method of claim 1 , wherein the chemolithoautotrophic bacteria is a Ralstonia eutropha bacteria.

3. The method of claim 1 , wherein the chemolithoautotrophic bacteria is resistant to reactive oxygen species.

4. The method of claim 1 , wherein the product is an alcohol.

5. The method of claim 1 , wherein the product is at least one of a fatty acid, an alkane, a polyhydroxyalkanoate, and an amino acid.

6. The method of claim 1 , wherein the solution includes a phosphate.

7. The method of claim 1 , further comprising continuously bubbling carbon dioxide through the solution.

8. The method of claim 1 , further comprising maintaining an isolated gas volume above a surface of the solution within a head space of a reactor chamber.

9. The method of claim 8 , further comprising replenishing the isolated gas volume to an original composition at one or more time intervals.

10. The method of claim 8 , wherein the isolated gas volume comprises primarily carbon dioxide.

11. The method of claim 1 , wherein splitting of the water is controlled to generate hydrogen at a rate equal to a rate of hydrogen consumption by the bacteria.

12. The method of claim 1 , wherein the solution is disposed in a reactor chamber.

13. The method of claim 8 , wherein maintaining the composition of the isolated gas volume within the head space maintains the concentration of the bioavailable nitrogen in the solution below the threshold nitrogen concentration.

14. The method of claim 1 , further comprising sensing a concentration of one or more gases dissolved in the solution, and controlling the concentration of the one or more gases dissolved in the solution based at least in part on the sensed concentration of the one or more gases dissolved in the solution.

15. The method of claim 14 , further comprising changing the concentration of the one or more gases dissolved in the solution to be within a predetermined range based at least in part on the sensed concentration of the one or more gases dissolved in the solution.

16. The method of claim 14 , wherein the one or more gases includes hydrogen, and further comprising changing a generation rate of hydrogen by the cathode and the anode to control the concentration of hydrogen in the solution.

17. The method of claim 14 , wherein the one or more gases dissolved in the solution includes nitrogen gas dissolved in the solution.

18. A method comprising:
splitting water using a cathode including a cobalt-phosphorus alloy and an anode including cobalt phosphate in a solution containing a chemolithoautotrophic bacteria to form hydrogen (H 2 ) and oxygen (O 2 ) in the solution, and wherein the solution includes bioavailable nitrogen.

19. The method of claim 18 , further comprising providing carbon dioxide (CO 2 ) in the solution.

20. The method of claim 18 , further comprising continuously bubbling carbon dioxide through the solution.

21. The method of claim 18 , further comprising maintaining the bioavailable nitrogen in the solution to below a threshold nitrogen concentration to control production of a product by the chemolitoautrophic bacteria.

22. The method of claim 18 , wherein the chemolithoautotrophic bacteria is a Ralstonia eutropha bacteria.

23. The method of claim 18 , wherein the chemolithoautotrophic bacteria is resistant to reactive oxygen species.

24. The method of claim 18 , wherein the product is an alcohol.

25. The method of claim 18 , wherein the product is at least one of a fatty acid, an alkane, a polyhydroxyalkanoate, and an amino acid.

26. The method of claim 18 , wherein the solution includes a phosphate.

27. The method of claim 18 , further comprising maintaining an isolated gas volume above a surface of the solution within a head space of a reactor chamber.

28. The method of claim 27 , further comprising replenishing the isolated gas volume to an original composition at one or more time intervals.

29. The method of claim 27 , wherein the isolated gas volume comprises primarily carbon dioxide.

30. The method of claim 27 , wherein maintaining the composition of the isolated gas volume within the head space maintains the concentration of the bioavailable nitrogen in the solution below a threshold nitrogen concentration.

31. The method of claim 18 , wherein splitting of the water is controlled to generate hydrogen at a rate equal to a rate of hydrogen consumption by the bacteria.

32. The method of claim 18 , wherein the solution is disposed in a reactor chamber.

33. The method of claim 18 , further comprising sensing a concentration of one or more gases dissolved in the solution, and controlling the concentration of the one or more gases dissolved in the solution based at least in part on the sensed concentration of the one or more gases dissolved in the solution.

34. The method of claim 33 , further comprising changing the concentration of the one or more gases dissolved in the solution to be within a predetermined range based at least in part on the sensed concentration of the one or more gases dissolved in the solution.

35. The method of claim 33 , wherein the one or more gases includes hydrogen, and further comprising changing a generation rate of hydrogen by the cathode and the anode to control the concentration of hydrogen in the solution.

36. The method of claim 33 , wherein the one or more gases dissolved in the solution includes nitrogen gas dissolved in the solution.

Assignees

• Harvard College

Inventors

• Colón Brendan Cruz
• Liu Chong
• Ziesack Marika
• Silver Pamela Ann
• Nocera Daniel G

Classifications

• C25B3/25

  Reduction · CPC title

• C12P7/02 Primary

  containing a hydroxy group · CPC title

• C25B11/04

  characterised by the material · CPC title

• C25B1/04

  by electrolysis of water · CPC title

• C25B15/08

  Supplying or removing reactants or electrolytes; Regeneration of electrolytes · CPC title