Yeast strains and method for the production of omega-hydroxy fatty acids and dicarboxylic acids
US-2016304913-A1 · Oct 20, 2016 · US
Method for producing medium chain diol
US11091741B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11091741-B2 |
| Application number | US-201615771799-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 27, 2016 |
| Priority date | Oct 27, 2015 |
| Publication date | Aug 17, 2021 |
| Grant date | Aug 17, 2021 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
The present invention relates to a method for producing medium chain diol and, more particularly to recombinant microorganisms in which fatty alcohol dehydrogenase and/or fatty alcohol oxidase genes on a ω-oxidative metabolism pathway are deleted, the fatty aldehyde dehydrogenase genes are optionally deleted, and β-oxidative metabolism pathway-related genes are deleted, and to a method for producing medium chain diol from fatty acid-derived alcohol or alkane by culturing the recombinant microorganisms. The recombinant microorganisms of the present invention can produce a high yield of medium chain diol by preventing further oxidation and β-oxidative metabolism of fatty alcohols.
First claim
Opening claim text (preview).
The invention claimed is: 1. A recombinant microorganism from which all of fatty alcohol dehydrogenase genes, fatty alcohol oxidase genes in an ω-oxidative metabolism pathway and fatty aldehyde dehydrogenase gene are completely deleted, and β-oxidative metabolism pathway-related genes are also deleted, wherein the fatty alcohol dehydrogenase genes are ADH1, ADH2, ADH3, ADH4, ADH5, ADH6, ADH7, ADH8, and FADH genes, and the recombinant microorganism is Yarrowia lipolytica . 2. The recombinant microorganism of claim 1 , wherein each of the ADH1, ADH2, ADH3, ADH4, ADH5, ADH6, ADH7, ADH8 and FADH genes comprises base sequences set forth in SEQ ID NOs: 1 to 9, respectively. 3. The recombinant microorganism of claim 1 , wherein the fatty alcohol oxidase gene is an FAO gene. 4. The recombinant microorganism of claim 3 , wherein the FAO gene comprises a base sequence set forth in SEQ ID NO: 10. 5. The recombinant microorganism of claim 1 , wherein the fatty aldehyde dehydrogenase genes are FALDH1, FALDH2, FALDH3, and FALDH4 genes. 6. The recombinant microorganism of claim 5 , wherein each of the FALDH1, FALDH2, FALDH3, and FALDH4 genes comprises base sequences set forth in SEQ ID NOs: 11 to 14, respectively. 7. The recombinant microorganism of claim 1 , wherein the β-oxidative metabolism pathway-related gene is an acyl-CoA oxidase gene. 8. The recombinant microorganism of claim 7 , wherein the acyl-CoA oxidase genes are ACO1, ACO2, ACO3, ACO4, ACO5, and ACO6 genes. 9. The recombinant microorganism of claim 8 , wherein each of the ACO1, ACO2, ACO3, ACO4, ACO5, and ACO6 genes comprises base sequences set forth in SEQ ID NOs: 15 to 20, respectively. 10. A method for producing a medium chain diol, comprising: (1) preparing the recombinant microorganism according to claims 1 ; and (2) treating the recombinant microorganism with a substrate to culture the recombinant microorganism. 11. The method of claim 10 , wherein the substrate is selected from the group consisting of a fatty acid-derived alcohol and alkane. 12. The method of claim 11 , wherein the fatty acid-derived alcohol is an alcohol having 5 to 30 carbon atoms. 13. The method of claim 11 , wherein the alkane is an alkane having 5 to 30 carbon atoms. 14. The method of claim 12 , wherein the medium chain diol is a diol compound having 5 to 30 carbon atoms.
Assignees
Inventors
Classifications
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US11091741B2 cover?
- The present invention relates to a method for producing medium chain diol and, more particularly to recombinant microorganisms in which fatty alcohol dehydrogenase and/or fatty alcohol oxidase genes on a ω-oxidative metabolism pathway are deleted, the fatty aldehyde dehydrogenase genes are optionally deleted, and β-oxidative metabolism pathway-related genes are deleted, and to a method for prod…
- Who is the assignee on this patent?
- Korea Res Inst Bioscience & Biotechnology
- What technology area does this patent fall under?
- Primary CPC classification C12N15/70. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 17 2021 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).