DNA barcoding primer for identifying cephalopoda and use thereof

Patent metadata

Field	Value
Publication number	US-11085075-B2
Application number	US-202015931082-A
Country	US
Kind code	B2
Filing date	May 13, 2020
Priority date	May 14, 2019
Publication date	Aug 10, 2021
Grant date	Aug 10, 2021

Abstract

The present invention provides a DNA barcode and method for identifying cuttlefishes, mainly depending on the diversity of mitochondrial DNA, including designing universal primers on conservative regions on both sides of the species-specific variable region, amplifying DNA from cuttlefishes, and sequencing PCR products to obtain DNA sequences, and identifying cuttlefish species based on the similarity by comparing with the species information in the database. The method is advantageous in that it can achieve efficient and specific amplification of DNA from such cephalopods as cuttlefishes, with low requirements for necessary instruments and simple operation processes. In addition, the method can be carried out in most molecular biology laboratories, which greatly improves the probability of success of molecular identification for cuttlefishes and sibling species thereof.

First claim

What is claimed is:

1. A method for identifying cephalopods, wherein the method comprises subjecting an individual to be detected to PCR amplification by using a DNA barcoding primer pair to produce an amplification product, sequencing the amplification product, calibrating or editing the sequence obtained after amplification and submitting the sequence obtained after calibrating or editing to the GenBank database for alignment, and identifying the species of the individual to be detected based on the similarity of DNA sequences; wherein the sequence of the upstream primer of the DNA barcoding primer pair is as shown in SEQ ID NO: 3, and the sequence of the downstream primer of the DNA barcoding primer pair is as shown in SEQ ID NO: 4.

2. The method according to claim 1 , wherein in the PCR amplification, the primer pair is annealed at 50° C.

Assignees

• Univ Jiliang China

Inventors

• Guan Feng
• Zhao Jin

Classifications

• C12Q1/6888 Primary

  for detection or identification of organisms · CPC title

• C12Q2565/537

  characterised by the capture oligonucleotide acting as a primer · CPC title

• C12Q1/686 Primary

  Polymerase chain reaction [PCR] · CPC title