Maintaining spectral quality over long measuring periods in imaging mass spectrometry

Patent metadata

Field	Value
Publication number	US-11081328-B2
Application number	US-202016782647-A
Country	US
Kind code	B2
Filing date	Feb 5, 2020
Priority date	Feb 8, 2019
Publication date	Aug 3, 2021
Grant date	Aug 3, 2021

Abstract

The invention relates to imaging mass spectrometry on thin sample sections, in particular using MALDI, where a high lateral image resolution means that a plethora of mass spectra has to be acquired and the image acquisition runs over many hours. The quality of the mass spectra deteriorates considerably over time in such cases. The invention is based on the finding that the decrease in spectral quality of continuous measurement series over many hours is only partially caused by a decrease in detector gain, and that another significant cause is a decrease in the number of usable ions per ion generating pulse, which is attributable to several phenomena that are difficult to regulate. The invention now proposes to instead regulate only the detector gain, and such that not only the decrease in the detector gain is compensated, but also the decrease in the number of usable ions per ion generating pulse.

First claim

The invention claimed is:

1. A method for imaging mass spectrometry on thin sample sections, from which a large number of mass spectra are continuously acquired in a mass spectrometer with ion detector via a pixel pattern in order to record a distribution of signals in the thin sample sections, where regulating an ion detector voltage enables ion currents, which are measured by the detector across a selected mass range in the mass spectra and summed, to be kept constant at a target value in the long term over the measurement of many mass spectra in order to maintain the quality of the mass spectra over a period of many hours until the end of the measurements.

2. The method according to claim 1 , wherein the ionization of molecules of the thin sample section is realized by matrix-assisted laser desorption (MALDI).

3. The method according to claim 2 , wherein the ion currents measured include at least a portion of the matrix ions.

4. The method according to claim 1 , wherein the mass spectra are acquired by a time-of-flight mass spectrometer, ion cyclotron resonance mass spectrometer, or mass filter.

5. The method according to claim 1 , wherein the ion currents measured represent an ion current over the complete mass spectrum (total ion count) or a part of it.

6. The method according to claim 1 , wherein a clock-pulse rate of the ion detector voltage regulation has a predetermined value at the start of the measurement and changes to a lower predetermined value over the further course of the measurement.

7. The method according to claim 1 , wherein the ion currents are determined by forming averages across several mass spectra.

8. The method according to claim 7 , wherein a sliding average is formed across several hundred to several thousand pixels, or wherein a series of averages across several hundred or several thousand pixels (“section averages”) in each case is formed.

9. The method according to claim 7 , wherein the ion detector voltage is virtually changed continuously with a predetermined temporal drift value during the acquisition of the mass spectra, and the drift value is corrected when the average of the ion currents no longer remains constant over time.

10. The method according to claim 9 , wherein a temporal constancy of the ion currents is determined by straight lines, which are applied to curves of the averages and whose gradient is used to calculate drift values.

11. The method according to claim 9 , wherein a derivative curve of the curves of the averages is formed by calculating differences between successive averages, wherein a distribution curve of the variances of this derivative curve is formed, and a deviation of the distribution centroid from zero is used to calculate an ion current drift correction.

12. The method according to claim 1 , wherein the signals in the thin sample section originate from peptides, lipids, phosphorylated molecules, pharmaceutical agents and/or composite markers for unusual tissue states such as carcinogenic degenerations.

13. The method according to claim 1 , wherein an acquisition rate for the mass spectra is in the kilohertz range, while around 10 to 1,000 mass spectra per pixel are added together to form a sum spectrum.

Assignees

• Bruker Daltonik Gmbh

Inventors

• Höhndorf Jens
• Haase Andreas

Classifications

• H01J49/164

  Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI] (sample holders H01J49/0418) · CPC title

• H01J49/0004 Primary

  Imaging particle spectrometry · CPC title

• H01J49/0036 Primary

  Step by step routines describing the handling of the data generated during a measurement · CPC title

• H01J49/40

  Time-of-flight spectrometers (H01J49/36 takes precedence) · CPC title

• H01J49/0027

  Methods for using particle spectrometers · CPC title